TGuide Smart Magnetic Tissue DNA Kit
Inombolo yekhathalogu / ukupakishwa
| Ikati. Hayi | ID | Inani lamalungiselelo |
| 4995038(ipleyiti) | DP602-E | 96 |
| 4993547(ikhatriji) | DP602-DE | 48 |
Isikhokelo sokukhetha i-Reagent ye-TGuide S16
| Nuclei Acid | Uhlobo lweSampuli | Igama lemveliso | Ubungakanani bokupakisha (preps) Ikhatriji/ ipleyiti | Inombolo yekati. Ikhatriji/ipleyiti |
| DNA | Inyama yezilwanyana | TGuide Smart Magnetic Tissue DNA Kit | 48/96 | 4993547/4995038 |
| DNA | Isityalo kunye neMbewu | TGuide Smart Magnetic Plant DNA Kit | 48 | 4993548 |
| DNA | Umhlaba/Isitulo | TGuide Smart Soil / Stool DNA Kit | 48 | 4993549 |
| DNA | Iimveliso zeGel & PCR | TGuide Smart DNA Ikhithi yokuCoca | 48 | 4993550 |
| DNA | IGazi Elipheleleyo | TGuide Smart Blood Genomic DNA Kit | 48/96 | 4993703/4995206 |
| DNA | Iseli/iSwab/Amabala omileyo, njalo njalo | TGuide Smart Universal DNA Kit | 48/96 | 4993704/4995040 |
| I-RNA | Igazi/Iseli/Izicubu | TGuide Smart Blood/Cell/Tissue RNA Kit | 48/96 | 4993551/4995043 |
| I-RNA | Isityalo kunye neMbewu | TGuide Smart Magnetic Plant RNA Kit | 48 | 4993552 |
| DNA/RNA | Ulwelo lwasimahla lweSeli (iSerum, njalo njalo) | TGuide Smart Viral DNA/RNA Kit | 48/96 | 4993702/4995207 |
Intshayelelo yemveliso
Hlanza i-DNA kwi-10-50 mg izicubu ngaphantsi kwemizuzu engama-50.
I-DNA ehlanjululweyo kunye nale kit iyasebenza kuluhlu lwezicelo ezisezantsi, kubandakanywa ukugaywa, i-PCR, ukwakhiwa kwethala leencwadi, ukuxutywa kwe-Southern, kunye nezinye iimvavanyo.
Umthamo we-elution oguquguqukayo ikuvumela ukuba wandise ukugxila okanye isivuno.
Iimbonakalo
Kulula kakhulu ukuyisebenzisa
Akukho msebenzi owongezelelweyo wokubhobhoza.
Umthamo we-elution unokuhlengahlengiswa njengoko kufuneka.
Ufakelo kunye nokusebenza kufuna uqeqesho oluncinci. Ngeenkqubo ezifakwe ngaphambili, khupha i-cartridge, khetha iprotocol kwaye usebenzise uvavanyo lwakho. Iinkqubo zikulungele ukusetyenziswa, kwaye ziyakwazi ukwenziwa.
Ii-reagents ezilayishwe kwangaphambili kunye nezinto ezisetyenziselwa ukulahla.
Ukuphathwa kweekhemikhali kuyapheliswa ukuya kwinqanaba elikhulu lokuqinisekisa ukhuseleko lokusebenza.
Iziphumo zeDemo
Ukutsalwa kweDNA yezilwanyana
Umphumo wovavanyo: i-DNA tissue genomic DNA ekhutshwe ngokuzenzekelayo yi-TGuide S16 exhotywe nge-reagent TGuide Smart Magnetic Tissue DNA Kit (4993547) inesivuno esiphezulu kunye nococeko, olulingana ne-DNA ekhutshwe yi-spin column-based extraction protocol. Ubunyulu ngokusisiseko bufana, kunye ne-OD260 / OD280 malunga ne-1.7 ~ 1.9 kunye ne-OD260 / OD230> 1.7. Ukuqukumbela, i-TGuide S16 ingasetyenziselwa ukukhupha i-DNA ye-genomic kwizicubu zezilwanyana endaweni yesisombululo se-spin-based based extraction solution.
Ukulayisha umthamo: 2 μl.
I-Agarose gel concentration: 1%. I-Electrophoresis yemizuzu engama-20 kwi-6 V / cm;
M:TIANGEN uMphawu III
I-1-4: intliziyo 5-8: i-spleen 9-12: imiphunga 13-16: izintso 17-20: ingqondo
Iisampulu ezimbini zokuqala zezicubu nganye zikhutshwe nge-spin-column based extraction kit, kwaye ezimbini zokugqibela zikhutshwe ngokuzenzekelayo yi-TGuide S16.
Umthombo wesampula: CD-1 iimpuku
