I-Evolutionary Genetics
Iinkonzo eziluncedo
Takagi et al.,Ijenali yezityalo, 2013
●Uhlalutyo olubanzi lwe-bioinformatic:ivumela uqikelelo lweyantlukwano yemfuza, ebonakalisa amandla okuzivelela kwezidalwa, kunye nokutyhila ubudlelwane obuthembekileyo bephylogenetic phakathi kweentlobo ezinempembelelo encitshisiweyo yenguquko eguquguqukayo kunye nendaleko ehambelanayo.
●Uphononongo olulungiselelweyo olukhethwayo: njengoqikelelo lwexesha lokwahlukana kunye nesantya esisekwe kwiiyantlukwano kwinqanaba le-nucleotide kunye ne-amino acids.
●UbuGcisa obuBanzi kunye neerekhodi zopapasho: I-BMKGene iqokelele amava amakhulu kuluntu kunye neeprojekthi ze-genetics ze-evolutionary genetics iminyaka engaphezu kwe-15, equka amawaka eentlobo zezilwanyana, njl. kwaye ibe negalelo kwiiprojekthi eziphakamileyo ze-1000 ezipapashwe kwi-Nature Communications, iZityalo zeMolekyuli, i-Plant Biotechnology Journal, njl.
● Iqela le-bioinformatics elinezakhono eziphezulu kunye nomjikelo wohlalutyo omfutshane: ngamava amnandi kuhlalutyo oluphambili lwe-genomics, iqela le-BMKGene linikezela ngohlahlelo olubanzi kunye nexesha elikhawulezayo lokuguqula.
● Inkxaso yasemva kwentengiso:Ukuzinikela kwethu kudlulela ngaphaya kokugqitywa kweprojekthi kunye neenyanga ezi-3 emva kokuthengiswa kwenkonzo. Ngeli xesha, sinikezela ngokulandelelana kweprojekthi, uncedo lokusombulula ingxaki, kunye neeseshoni ze-Q&A ukujongana nayo nayiphi na imibuzo enxulumene neziphumo.
IiNkcazo zeNkonzo kunye neemfuno
| Uhlobo lolandelelwano | Umlinganiselo wabemi ocetyiswayo | Isicwangciso sokulandelelana | Iimfuno zeNucleotide |
| Ulandelelwano lweGenome epheleleyo | ≥ 30 abantu, kunye ≥ 10 abantu ngabanye kwiqela elincinane ngalinye
| 10x | Ugxininiso: ≥ 1 ng/ µL Imali iyonke≥ 30ng Umda okanye akukho ukuthotywa okanye ukosuleleka |
| Iqhekeza leNdawo Ekhethekileyo-Eyandisiweyo (SLAF) | Ubunzulu bethegi: 10x Inani leethegi: <400 Mb: I-WGS iyacetyiswa <1Gb: 100K iithegi 1Gb > 2Gb: 300K iithegi Ubuninzi bethegi ezingama-500k | Ugxininiso ≥ 5 ng/µL Imali iyonke ≥ 80 ng Nanodrop OD260/280=1.6-2.5 Ijeli yeAgarose: akukho okanye ukuthotywa okulinganiselweyo okanye ungcoliseko
|
Ukuhamba komsebenzi weNkonzo
Uyilo lovavanyo
Ukuhanjiswa kwesampuli
Ukwakhiwa kwethala leencwadi
Ukulandelelanisa
Uhlalutyo lwedatha
Iinkonzo emva kokuthengisa
Inkonzo ibandakanya uhlalutyo lwesakhiwo sabemi (umthi we-phylogenetic, i-PCA, itshathi yohlulo lwabantu), ukuhlukahluka kwabemi, kunye nokukhethwa kwabantu (i-linkage disequilibrium, ukhetho lokutshayela olukhethiweyo lweendawo ezinenzuzo). Inkonzo inokubandakanya uhlalutyo olulungiselelweyo (umz. ixesha lokwahlukana, ukuhamba kwemfuza).
*Iziphumo zedemo eziboniswe apha zonke zivela kwiigenomes ezipapashwe nge-BMKGENE
I-1.Uhlalutyo lwe-Evolution luqulethe ukwakhiwa komthi we-phylogenetic, isakhiwo sabantu kunye ne-PCA esekelwe kwiinguqu zofuzo.
Umthi we-Phylogenetic umele ubudlelwane be-taxonomic kunye ne-evolutionary phakathi kweentlobo kunye nezinyanya eziqhelekileyo.
I-PCA ijolise ekuboneni ukusondelana phakathi kwabantu abancinci.
Ubume benani labantu bubonisa ubukho benani labemi abahlukileyo ngokwemfuza ngokwemiqukumbelo yeallele.
Chen, kunye. al.,PNAS, 2020
2.Ukutshayela okukhethiweyo
Ukutshayela okukhethiweyo kubhekiselele kwinkqubo apho isiza esinenzuzo sikhethwa kwaye ukuphindaphindwa kweendawo ezingathathi hlangothi zinyuswa kwaye ezo sayithi zingadityaniswanga ziyancipha, okukhokelela ekuncipheni kommandla.
Ubhaqo olubanzi lwe-Genome kwimimandla ekhethiweyo yokutshayela kusetyenzwa ngokubala isalathisi semfuza yabemi（π，Fst, Tajima's D) yazo zonke ii-SNP ngaphakathi kwefestile etyibilikayo (100 Kb) kwinqanaba elithile (10 KB).
Ukwahluka kwe-Nucleotide (π)
UTajima D
Ukulungiswa kwesalathiso(Fst)
Wu, kunye. al.,Isityalo seemolekyuli, 2018
3.Gene Flow
Wu, kunye. al.,Isityalo seemolekyuli, 2018
Imbali ye-4.Demographic
Zhang, kunye. al.,I-Ecology yendalo kunye ne-Evolution, 2021
5.Ixesha leDivergence
Zhang, kunye. al.,I-Ecology yendalo kunye ne-Evolution, 2021
Phonononga inkqubela phambili eququzelelwa yi-BMKGene yeenkonzo zemfuzo ngengqokelela egciniweyo yopapasho:
Hassanyar, AK et al. (2023) 'Ukufunyanwa kweempawu ze-SNP zeMolekyuli kunye neGenome yoMviwa eNxulunyaniswe ne-Sacbrood Virus Resistance kwi-Apis cerana cerana Larvae yi-Whole-Genome Resequencing',Ijenali yamazwe ngamazwe yenzululwazi yeemolekyuli, 24(7). doi: 10.3390/IJMS24076238.
Chai, J. et al. (2022) 'Ukufunyaniswa kwendawo yasendle, esulungekileyo yaseTshayina intulo enkulu kudala amathuba amatsha olondolozo',Uphando lweZoological, 2022, Umqu. 43, ukukhutshwa kwe-3, amaphepha: 469-480, 43 (3), iphepha 469-480. doi: 10.24272/J.ISSN.2095-8137.2022.101.
UHan, M. et al. (2022) 'Ipateni yePhylogeographical kunye neMbali yeNdalo yaBemi yeMveli ye-Elymus sibiricus L. eQinghai-Tibetan Plateau',Imida kwiNzululwazi yeZityalo, 13, iphe. 882601. doi: 10.3389/FPLS.2022.882601/BIBTEX.
Wang, J. et al. (2022) 'Iingcinga zeGenomic kwi-longan evolution ukusuka kwindibano yegenome yenqanaba lechromosome kunye negenomics yabemi bexesha elide',Uphando lweHorticulture, 9. doi: 10.1093/HR/UHAC021.
