I-Eukaryotic mRNA Sequencing-NGS
Iimbonakalo
● Ukuthatyathwa kwepoly mRNA phambi kokulungiswa kwethala leencwadi
● Ulungiselelo lwethala leencwadi le-mRNA elinokuzikhethela ukwenza ukuba kufunyanwe idatha yolandelelwano lwestrand-specific
● Uhlalutyo lwe-Bioinformatic ye-gene expression kunye ne-transcript structure
Iingenelo
●UbuGcisa obuBanzi: Iqela lethu lenze ngaphezulu kweesampulu ezingama-600,000 kwi-BMKgene, ezithatha iintlobo ngeentlobo zeesampulu ezifana neenkcubeko zeeseli, izicubu kunye nolwelo lomzimba. Sizisa ubutyebi bamava kwiprojekthi nganye kwaye sigqibe ngempumelelo ngaphezulu kwe-100,000 yeeprojekthi ze-mRNA-Seq kwiindawo ezahlukeneyo zophando.
●Ulawulo loMgangatho olungqongqo: Sisebenzisa iindawo zolawulo ezingundoqo kuzo zonke izigaba, ukusuka kwisampulu kunye nolungiselelo lwethala leencwadi ukuya kulandelelwano kunye ne-bioinformatics. Olu hlolo lucokisekileyo luqinisekisa ukuhanjiswa kweziphumo ezikumgangatho ophezulu rhoqo, kukuqinisekisa ukuba iprojekthi yakho isezandleni ezithembekileyo.
●Isichasiselo esiBanzi: Sisebenzisa i-database ezininzi ukuze sichaze ngokusebenzayo i-Differentially Expressed Genes (DEGs) kwaye senze uhlalutyo oluhambelanayo lokutyebisa. Le ndlela ibanzi ibonelela ngokuqonda kwiinkqubo zeseli kunye neemolekyuli eziphantsi kwempendulo ye-transcriptome, eqinisekisa ukuba unolwazi olupheleleyo malunga neziphumo zeprojekthi yakho.
●Inkxaso yePost-Sales: Ukuzinikela kwethu kudlulela ngaphaya kokugqitywa kweprojekthi kunye neenyanga ezi-3 emva kokuthengiswa kwenkonzo. Ngeli xesha, sinikezela ngokulandelelana kweprojekthi, uncedo lokusombulula ingxaki, kunye neeseshoni ze-Q&A ukujongana nayo nayiphi na imibuzo enxulumene neziphumo.
IiMfuno zeSampulu kunye nokuhanjiswa
| Ithala leencwadi | Isicwangciso sokulandelelana | Idatha iyacetyiswa | Ulawulo lwemeko |
| UPoly A utyebile | Illumina PE150 I-DNBSEQ-T7 | 6 -10 Gb | Q30≥85% |
IiMfuno zeSampulu:
IiNucleotides:
| Conc.(ng/μl) | Imali (μg) | Ubunyulu | Ingqibelelo | |
| Ithala leencwadi elisemgangathweni | ≥ 10 | ≥ 0.2 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Unyino okanye akukho protein okanye ungcoliseko lweDNA olubonakaliswe kwijeli. | Kwizityalo: RIN≥4.0; Kwizilwanyana: RIN≥4.5; 5.0≥28S/18S≥1.0; umphakamo osisiseko okanye ongekhoyo |
| Ithala leencwadi elingumkhombandlela | ≥ 10 | ≥ 0.2 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Unyino okanye akukho protein okanye ungcoliseko lweDNA olubonakaliswe kwijeli. | Kwizityalo: RIN≥4.0; Kwizilwanyana: RIN≥4.5; 5.0≥28S/18S≥1.0; umphakamo osisiseko okanye ongekhoyo |
● Izityalo:
Ingcambu, Isiqu okanye iPetal: 450 mg
Igqabi okanye Imbewu: 300 mg
Isiqhamo: 1.2 g
●Isilwanyana:
Intliziyo okanye Amathumbu: 300 mg
Viscera okanye Brain: 240 mg
Izihlunu: 450 mg
Amathambo, Iinwele okanye Ulusu: 1g
● IiArthropods:
Izinambuzane: 6g
I-crustacea: 300 mg
● Igazi elipheleleyo: ityhubhu e-1
● Iiseli: 106 iiseli
Unikezelo lweSampuli eCetyisiweyo
Isikhongozeli: 2 ml centrifuge ityhubhu (Ifoyile yeTin ayikhuthazwa)
Isampuli yokuleyibhela: Iqela+liphindaphinda umz. A1, A2, A3; B1, B2, B3....
Ukuthunyelwa ngenqanawa:
1. Umkhenkce owomileyo: Iisampulu kufuneka zipakishwe kwiingxowa kwaye zingcwatywe kumkhenkce owomileyo.
2. Iityhubhu zetafile ze-RNAs: Iisampulu ze-RNA zingomiswa kwi-tube ye-RNA yokuzinzisa (umzekelo, i-RNAstable®) kwaye ihanjiswe kwiqondo lokushisa.
Ukuhamba komsebenzi weNkonzo
Uyilo lovavanyo
Ukuhanjiswa kwesampuli
Ukukhutshwa kwe-RNA
Ukwakhiwa kwethala leencwadi
Ukulandelelanisa
Uhlalutyo lwedatha
Iinkonzo emva kokuthengisa
Bioinformatics
I-Eukaryotic mRNA ulandelelwano lokuhlalutya ukuhamba komsebenzi
Bioinformatics
ØUlawulo lomgangatho wedatha ekrwada
ØReference genome ulungelelwaniso
ØUhlalutyo lwesakhiwo esibhaliweyo
ØUbungakanani benkcazo
ØUhlalutyo lwentetho eyahlukileyo
ØInkcazo yomsebenzi kunye nokutyebisa
Reference Genome Ulungelelwaniso
Ukugcinwa kwedatha
Ulungelelwaniso lwesampulu kunye novavanyo lweereplicate zebhayoloji
Iimfuza ezivezwe ngokwahlukileyo (DEGs)
Inkcazo esebenzayo yee-DEGs
Uphuculo oluSebenzayo lweeDEGs
Phonononga inkqubela phambili eququzelelwa yi-BMKGene's eukaryotic NGS mRNA iinkonzo zolandelelwano ngengqokelela egciniweyo yopapasho.
Huang, L. et al. (2023) 'I-Triclosan kunye ne-triclocarban zenza buthathaka umthamo wokunuka kwe-goldfish ngokunyanzelwa ukuqondwa kwevumba, ukuphazamisa ukuhanjiswa komqondiso onukayo, kunye nokuphazamiseka kokwenziwa kolwazi oluvumbayo', UPhando lwaManzi, 233, iphe. 119736. doi: 10.1016/J.WATRES.2023.119736.
Jia, LJ et al. (2023) 'Aspergillus fumigatus hijacks human p11 to redirect fungal-containing phagosomes to non-degradative pathway', Cell Host & Microbe, 31(3), pp. 373-388.e10. doi: 10.1016/J.CHOM.2023.02.002.
Jin, K. et al. (2022) 'Iizinto ze-TCP zokuBhala eziBandakanyiweyo kwi-Shoot Development ye-Ma Bamboo (i-Dendrocalamus latiflorus Munro)', i-Frontiers kwiSayensi yeZityalo, i-13, iphe. 884443. doi: 10.3389/FPLS.2022.884443/BIBTEX.
Wen, X. et al. (2022) 'I-Chrysanthemum lavandulifolium genome kunye ne-molecular mechanism ephantsi kweentlobo ezahlukeneyo ze-capitulum', i-Horticulture Research, 9. doi: 10.1093/HR/UHAB022.
UZhang, uYujie et al. (2023) 'I-cascade nanoreactor yokuphucula unyango lwe-sonodynamic kumhlaza we-colorectal nge-synergistic ROS augment kunye nokuvalwa kwe-autophagy', Nano Namhlanje, 49, iphe. 101798. doi: 10.1016 / J.NANTOD.2023.101798.
