IIMPAPASHO ZEPHONZO ZE-DNBSEQ
Iimbonakalo
●Iplatifomu:I-MGI-DNBSEQ-T7
●Iindlela zokulandelela:Pe150
●Ukudluliselwa kweelayibrari ze-IllinaMgi:Ukwenza i-sequedation yemitha yedatha ephezulu ngeendleko eziphantsi.
●Ukulawulwa komgangatho wamathala eencwadi ngaphambi kokulandelelana.
●Ukulandelelana kwedatha ye-QC kunye nokuHanjiswa:Ukuhanjiswa kwengxelo ye-QC kunye nedatha eluhlaza kwifomathi ye-Fastq ngokukhawuleza emva kokuphononongwa komdaniso kwaye ukucoca i-Q30 kufunda.
Izibonelelo
●Ukusebenza kweenkonzo zokulandelelana:Umthengi unokukhetha ukulandelelana kwendlela okanye isixa sedatha.
●Iziphumo eziPhakamileyo:I-1500 GB / indlela
●Ukuhanjiswa kwengxelo ye-QC ye-QC:Nge-metrics esemgangathweni, ukuchaneka kwedatha kunye nokusebenza ngokubanzi kweprojekthi yokulandelelana.
●Inkqubo ye-lequeeding eqolileyo:ngokujika okufutshane.
●Ulawulo oluqhelekileyo lomgangatho: Sisebenzisa iimfuno ezingqongqo ze-QC ukuqinisekisa ukuhanjiswa kweziphumo ezisemgangathweni eziphezulu.
Iifoto zeSampula
| Isixa sedatha (x) | Uxinzelelo (QPCR / NM) | Ivolumu | |
| Indlela yenxalenye
| X ≤ i-10 GB | ≥ 1NM | ≥ 25 μl |
| I-10 GB <X ≤ i-50 GB | ≥ 2 NM | ≥ 25 μl | |
| I-50 GB <X ≤ i-100 GB | ≥ 3 nm | ≥ 25 μl | |
| X> I-100 GB | ≥ 4 nm | ||
| Indlela enye | Ngawe ndlela | ≥ 1.5 NM / iphuli yethala leencwadi | ≥ I-25 μl / iphuli yethala leencwadi |
Ukongeza kuxinzelelo kunye nenani lilonke, inkangeleko efanelekileyo ye-piaki nayo iyafuneka.
Qaphela: Indlela yokulinganisa yeelayibrari eziphantsi ze-hix ifuna i-hix spike-ukuba iqinisekise ukufowuna.
Sicebisa ukuba singenise amathala eencwadi athunyelweyo njengesampulu. Ukuba ufuna i-bmkgene ukwenza i-chaolling yethala leencwadi, nceda ujonge kwi
Iimfuneko zelayibrari zendlela yokutsala inxenye.
Ubungakanani bethala leencwadi (Imephu yepik)
I-Peak enkulu kufuneka ibe ngaphakathi kwe-300-450 bp.
Iilayibrari kufuneka zibe nencopho enye eyintloko, akukho ngcoliseko ye-adaptha kwaye akukho dimer primers.
Inkonzo yenkonzo
Ingxelo yeThala leeNcwadi ye-QC
Ingxelo malunga nomgangatho wethala leencwadi ibonelelwe ngaphambi kokuba i-sequemedeng, ivavanya inani lethala leencwadi, kunye nokuqhekeka.
Ukulandelela kwe-QC Ingxelo
Itheyibhile 1. Iinkcukacha-manani kwidatha yokulandelelana.
| Isampula yesazisi | Bmkid | I-Raw ifundwa | Idatha eluhlaza (BP) | I-Recon STACS (%) | I-Q20 (%) | I-Q30 (%) | I-GC (%) |
| C_01 | Bmk_01 | I-22,870,120 | I-6,861,036,000 | I-96.48 | 99.14 | I-94.85 | 36.67 |
| C_02 | Bmk_02 | I-14,717,867 | I-4,415,360,100 | I-96.00 | I-98.95 | I-93.89 | 37.08 |
Umzobo 1. Ukusasazwa komgangatho kunye nokufunda kwisampulu nganye
Umzobo 2. Isiseko somxholo wesiseko
Umzobo 3. Ukuhanjiswa kwemixholo yokufunda kwi-leceneding idatha
