Illumina amathala eencwadi enziwe kwangaphambili
Iimbonakalo
●Amaqonga:Illumina NovaSeq 6000 kunye NovaSeq X Plus
●Iimowudi zolandelelwano:PE150 kunye ne-PE250
●Ukulawulwa komgangatho wamathala eencwadi phambi kolandelelwano
●Ukulandelelanisa idatha ye-QC kunye nokuhanjiswa:ukuhanjiswa kwengxelo ye-QC kunye nedatha ekrwada kwifomathi ye-fastq emva kwe-demultiplexing kunye nokucoca ukufundwa kwe-Q30
Iinkonzo eziluncedo
●Ukuguquguquka kweenkonzo zolandelelwano:umthengi unokukhetha ukulandelelanisa ngelayini, i-flow cell, okanye ngenani ledatha efunekayo (inxalenye yolandelelwano lwendlela).
●Amava abanzi kwiqonga lokulandelelana kwe-Illumina:ngamawaka eeprojekthi ezivaliweyo ezineentlobo ezahlukeneyo.
●Ukuhanjiswa kolandelelwano lwengxelo ye-QC:kunye neemetrics ezisemgangathweni, ukuchaneka kwedatha kunye nokusebenza ngokubanzi kweprojekthi yokulandelelana.
●Inkqubo yolandelelwano lwabantu abadala:ngexesha elifutshane lokujika.
●Ulawulo loMgangatho olungqongqo: siphumeza iimfuno ezingqongqo ze-QC ukuqinisekisa ukuhanjiswa kweziphumo ezihlala zikumgangatho ophezulu.
Iiplatform zesampuli
| Iqonga | Iseli equkuqelayo | Imo yolandelelwano | Iyunithi | Isiphumo esiqikelelweyo |
| NovaSeq X | 10B (iindlela ezisi-8) | PE150 | Indlela enye Indlela Engaphelelanga | 375Gb / Indlela |
| 25B (iindlela ezisi-8) | PE150 | Indlela enye Indlela Engaphelelanga | 1000 Gb/Ndlela | |
| NovaSeq 6000 | Iseli ye-SP Flow (iindlela ezi-2) | PE250 | Iseli equkuqelayo Indlela enye Indlela Engaphelelanga | 325-400 M ifundeka / Lane |
| S4 Flow cell (4 imizila) | PE150 | Iseli equkuqelayo Indlela enye Indlela Engaphelelanga | ~800Gb / Indlela |
IiMfuno zeSampulu
| Isixa seDatha (X) | Ugxininiso (qPCR/nM) | Umthamo | |
| Ulandelelwano lweNdlela engaphelelanga
| X ≤ 10 Gb | ≥ 1 nM | ≥ 25 μl |
| 10 Gb < X ≤ 50 Gb | ≥ 2 nM | ≥ 25 μl | |
| 50 Gb < X ≤ 100 Gb | ≥ 3 nM | ≥ 25 μl | |
| X > 100 Gb | ≥ 4 nM | ≥ 25 μl | |
| Ulandelelwano lweNdlela | NgeNdlela nganye | ≥ 1.5 nM / Ichibi lethala leencwadi | ≥ 25 μl / ichibi lethala leencwadi |
Ukongeza kugxininiso kunye nenani elipheleleyo, ipateni efanelekileyo yokuphakama nayo iyafuneka.
Qaphela: Ulandelelwano lwendlela yamathala eencwadi ahlukeneyo asezantsi kufuna i-PhiX spike-in ukuqinisekisa ukuba umnxeba osisiseko owomeleleyo.
Sicebisa ukuba kungeniswe amathala eencwadi adityaniswe kwangaphambili njengeesampuli. Ukuba ufuna i-BMKGENE yenze ukudityaniswa kwethala leencwadi, nceda ujonge ku
iimfuno zethala leencwadi zolandelelwano lwenxalenye yendlela.
Ubungakanani beThala leencwadi (imephu encopho)
Incopho ephambili kufuneka ibe phakathi kwe-300-450 bp.
Amathala eencwadi kufuneka abe nencopho enye ephambili, kungabikho ungcoliseko lwe-adaptha kwaye kungabikho iiprimer dimers.
Nceda ufikelele kuthi ukuba iisampulu zakho azifikeleli kwiimfuno zemathiriyeli zokuqalisa.
Ukuhanjiswa kwenkonzo
Ulawulo lomgangatho wethala leencwadi
Ukulandelelanisa
Ulawulo lomgangatho wedatha
Unikezelo lweprojekthi
Ingxelo yeThala leeNcwadi le-QC
Ingxelo engomgangatho wethala leencwadi inikwa phambi kolandelelwano, uvavanyo lwesixa-mali sethala leencwadi, nokwahlulwa.
Ukulandelelanisa ingxelo ye-QC
Itheyibhile 1. Iinkcukacha-manani ngokulandelelana kwedatha.
| Isampulu yesazisi | BMKID | Ufunda ekrwada | Idatha ekrwada (bp) | Ufundo olucocekileyo (%) | Q20(%) | Q30(%) | GC(%) |
| C_01 | BMK_01 | 22,870,120 | 6,861,036,000 | 96.48 | 99.14 | 94.85 | 36.67 |
| C_02 | BMK_02 | 14,717,867 | 4,415,360,100 | 96.00 | 98.95 | 93.89 | 37.08 |
Umzobo 1. Ukuhanjiswa komgangatho kunye nokufundwa kwisampulu nganye
Umzobo 2. Isiseko sokuhanjiswa komxholo
Umzobo 3. Ukuhanjiswa kwemixholo efundiweyo ngokulandelelana kwedatha
