Ubude obugcweleyo MRNA
Iimbonakalo
● I-CDNA Synthesis evela kwiPoly-A i-MANNA ilandelwa kukulungiselela ithala leencwadi
● Ukulandelelana kwimowudi ye-CCS, ukuvelisa i-hifi
● Ukulandelelana kobude obugcweleyo
● Uhlalutyo alunyanzelekanga ukuba lube yireferensi; Nangona kunjalo, inokuqeshwa
● Uhlalutyo lwe-biorionacic lwenza ukuba uhlalutyo lwe-Isoforcriplot Lncrn lncrna, i-gene fusions, i-poly-diencyred, isakhiwo semfuza
Izibonelelo zeNkonzo
●Ukuchaneka okuphezulu: I-HIFI ifunda ngokuchaneka> 99.9% (Q30), ngokuthelekiswa ne-NGS
● Uhlalutyo olungeyo: Ukulandelelana kwako konke ukuhanjiswa kwenza inkcazo ye-Isfow kunye nophawu
●Ubuchule obubanzi: Ngerekhodi yengoma yokugcwalisa ngaphezulu kwe-1100 ye-pacbio egcweleyo ye-pacbiome epheleleyo kunye neesampulu ezingaphezu kwama-2300, iqela lethu lizisa ubutyebi bamava kuzo zonke iiprojekthi.
●Inkxaso yasemva kwentengiso: Ukuzinikela kwethu kugqitha ngaphaya kokugqitywa kweprojekthi kunye nexesha lenkonzo yenkonzo yasemva kwenkonzo emva kwenkonzo. Ngeli xesha, sinikezela ngeprojekthi yokulandela iprojekthi, uncedo lokucombulula iingxaki, kunye ne-Q & Iiseshoni zokujongana nayo nayiphi na imibuzo enxulumene neziphumo.
IiMfuno kunye nokuHanjiswa
| Ithala leencwadi | Isicwangciso sokulandelelana | Idatha eyacetyiswayo | Ulawulo lwemeko |
| I-Polya inika ilayibrari yePolya CS | I-Pacbio Sequel II I-Pacbio Revio | I-20/40 GB I-5/10 m Ccs | I-Q30≥85% |
Iifoto zeSampula:
I-NuCuotides:
● Izityalo:
Ingcambu, i-stem okanye petal: 450 mg
Igqabi okanye imbewu: 300 mg
Iziqhamo: 1.2 g
● Isilwanyana:
Intliziyo okanye iesine: 300 mg
I-Vicera okanye ingqondo: I-240 mg
Imisipha: 450 mg
Amathambo, iinwele okanye ulusu: 1g
● I-Arthropods:
Izinambuzane: I-6G
I-crustacea: 300 mg
● Igazi liphela: I-1 ityhubhu
● Iiseli: 106 iiseli
| I-TRI. (G / μl) | Isixa (μg) | Ubunyulu | Ingqibelelo |
| 100 | ≥ 1.0 | I-OD260 / 280 = 1.7-2.5 I-OD260 / 230 = 0.5-2.5 I-protein okanye iprotein okanye ungcoliseko lwe-DNA oluboniswe kwi-Gel. | Kwizityalo: rin≥7.5; Kwizilwanyana: rin≥8.0; 5.0≥ 28s / 18s≥1.0; Inqunu okanye akukho kukuphakama kwesiseko |
Ukuhanjiswa kwesampulu
Isikhongozeli: I-2 Ml Centrifuge Tube (i-tin foil ayikhuthazwanga)
I-A1 yeQela + umz. A1, A2, A3; B1, B2, B3.
Ukuhanjiswa:
I-1. I-ice-yowomileyo: Iisampulu kufuneka zipakishwe ezingxoweni kwaye zingcwatyelwe kwi-ice eyomileyo.
I-2. Ii-tubs ezinobungozi: Iisampulu ze-RNNNA zinokomiswa kwi-tube yokuzinzisa i-RNA (umzekelo: i-rnastast®) kwaye ithunyelwe kubushushu begumbi.
Ukuhamba kwenkonzo
Uyilo lovavanyo
Ukuhanjiswa kwesampula
Ukukhutshwa kwe-RNA
Ulwakhiwo lwethala leencwadi
Ukulandelelana
Uhlalutyo lwedatha
Iinkonzo zentengiso emva
Kubandakanya nohlalutyo olulandelayo:
● Ulawulo loMgangatho wedatha
● Uhlalutyo olungeThintelo lwePolyadyyer (APA)
● Uhlalutyo lwe-Fusion
● Uhlalutyo olungeyo
● Uhlalutyo lwe-Benchmark
● Uhlalutyo lwesicatshulwa se-Novel: Uxelelo lokulandelelana kwekhowudi (CDS) kunye nokuchazwa
● Uhlalutyo lwe-LNNCHNA: Uqikelelo lwe-lncrna kunye neethagethi
● Ukuchongwa kwe-Microsatelite (SSR)
Uhlalutyo lweBusco
Olunye uhlalutyo
Olunye uhlalutyo lwePolyadyyting (APA)
Umsebenzi wokunyanzeliswa kwe-Noves Recrippts
Jonga i-veractions eququzeleleke yi-Bmkgene's Nanopore egcwele iinkonzo ezigcweleyo kule mpapasho ibonisiweyo.
M, Y. Et Al. . I-110709. Doi: 10.1016 / j.ygeno.20231070709.
Chao, Q. ET AL. . Doi: 10.1111 / PBI.12958.
Deng, h. et al. . I-5808. Doi: 10.3390 / IJMS23105808 / s1.
I-Hua, X. ET Al. . Doi: 10.1038 / S42003-022-03000-Z.
Liu, m. et al. . 363. Doi: 10.3390 / I-Insects14040363 / S1.
Wang, Lijin et al. . DoI: 10.1186 / S1264--019-5832-9.
