Uhlalutyo oluxhaphakileyo
Izibonelelo zeNkonzo
I-Takagi et al., Ijenali yesityalo, ka-2013
● Ingingqi echanekileyo: Ukuxuba ii-busks ngama-30 + 30 ukuya kwi-200 + abangama-200 abantu ukuze banciphise ingxolo yemvelaphi; I-Soutonymous ye-Unicatetal-esekwe kwingingqi yengingqi.
● Uhlalutyo olubanzi: I-Exeth Heatate Umsebenzi we-Geetionation, kubandakanya i-NR, i-NR, iSwissisPrity, yiya, i-kegg, i-kegg, i-Kog, njl.
● Ukujika ngokukhawuleza kwexesha: Ingingqi ekhawulezileyo yemfuza kwiintsuku ezingama-45 zomsebenzi.
● Amava abalulekileyo: I-BMK inegalelo kumawakawaka eempawu zalapha ekhaya, ezigubungela iintlobo ezahlukeneyo njengezityalo, iimveliso zamanzi amaninzi, i-Aquatic, ihlathi, iziqhamo, njl njl.
Inkcazo yenkonzo
Inani labemi:
Ukwahlula inzala yabazali ngokuchaseneyo.
I-ETM. F2 I-PREGENY, I-BRCRUSSOSOST (BC), i-Ibbinant Line (RIL)
Ukuxuba iphuli
Kwimigqaliselo efanelekileyo: I-30 ukuya kwi-50 yabantu (ubuncinci be-20) / bulk
I-Tratitivery ye-Tratitive: I-5% ukuya kwi-10% yabantu abanemifanekiso egqithileyo kubo bonke abantu (ubuncinci abangama-30 + 30).
Ukucetyiswa ubunzulu bobunzulu
Ubuncinci i-20x / umzali kunye ne-1x / i-ofisi nganye (umz. I-SIDSTERORS exhotyisiweyo ye-30 + 30 umntu ngamnye, ukugcwala kuya kuba yi-30x nge-30x nge-30x nge-30x nge-bulk nganye)
I-bioinformatics ihlalutyo
● I-genome yonke
● Ukulungiswa kwedatha
● I-SNP / I-I-I-I-Indel
● Ukuvavanywa kommandla
● Umvavanyi umsebenzi wemfuza
IiMfuno kunye nokuHanjiswa
Iifoto zeSampula:
I-NuCuotides:
| isampulu yeGdna | Isampulu ye-TICESE |
| Uxinzelelo: ¶30 ng / μl | Izityalo: 1-2 g |
| Isixa: ≥2 μg (i-Hloughm ¶15 μ5 μl) | Izilwanyana: 0.5-1 g |
| Ucoceko: I-OD260 / 280 = 1.6-2.5 | Igazi liphela: 1.5 ML |
Ukuhamba kwenkonzo
Uyilo lovavanyo
Ukuhanjiswa kwesampula
Ukukhutshwa kwe-RNA
Ulwakhiwo lwethala leencwadi
Ukulandelelana
Uhlalutyo lwedatha
Iinkonzo zentengiso emva
Isiseko sohlalutyo lwe-1.astionation kwisiseko se-Euclidean Umgama (Ed) Ukuchonga indawo yomkhosi. Kumzobo olandelayo
I-X-Axis: Inombolo yeChromomontle; I-DOT nganye imele ixabiso le-snp. Umgca omnyama uhambelana nexabiso elipheleleyo le-Ed. Ixabiso eliphezulu eli-Ed libonisa umbutho obalulekileyo phakathi kwendawo kunye ne-phenotype. Umgca obomvu wedash umele umbundu woMbutho obalulekileyo.
Uhlalutyo lwe-2.associmations olusekwe kwi-SNP-Index
I-X-Axis: Inombolo yeChromomontle; I-DOT nganye imele ixabiso le-SNP-Index. Umgca omnyama umi kwixabiso le-snp le-snp. Ixabiso elikhulu kukuba, owona mbutho ubalulekileyo.
Ityala leBMK
I-fit enkulu ye-fit fit stushes ye-FNL7.1 edibanisa iProtein ye-Abryogenesis ye-Abryogenes ehambelana nobude bentamo yeziqhamo kwi-cucumber
Ipapashwe: Ijenali yeBiotechnology, 2020
Isicwangciso sokulandelelana:
Abazali (Jin5-508, YN): I-genome iyonke i-34 genome eyayisekiswa ne-34 × ne-20 × 20 ×.
I-DNA Pools (50 i-50 ineendlela ezingama-50 kwaye ezingama-50 zimfutshane): Ukuphinda-phinda i-61 × ne-52 × ne-52 ×
Iziphumo eziphambili
Kolu phononongo, ukwahlula-hlula kwabantu (F2 no-F2: 3) baveliswa ngokuwela i-cucumber ye-cucumber ende cucumber jin5-508 kunye nentsundu emfutshane yn. Iiphuli ezimbini zeDNA zakhiwe ngabantu abangama-50 entamo ende kunye nabantu abangama-50 emfutshane. Impembelelo enkulu ye-QTL yachongwa kwi-CRTA07 ngohlalutyo lwe-BSA kunye nemephu ye-qtl yendabuko ye-qtl. Ingingqi yomgqatswa yayiye yancitshiswa ngemephu ye-mephu, intetho ye-TLE Ukongeza, i-polymorphism e-CSFNL7.1 ingingqi yokukhuthaza ifunyenwe inxulunyaniswa nentetho ehambelana nayo. Uhlalutyo olungaphaya kwe-phylogenetice lucetyise ukuba i-FNL7.1 I-COOTIS inokwenzeka ukuba ivela eIndiya.
 I-QTL-Mapping kwi-BSA uhlalutyo lokuchonga indawo yokujonga umgqatswa ohambelana nobude be-cucumumber yentamo |  Iprofayili ye-cucumber intamo ye-qtl echongwe kwi-CHR07 |
Xu, x., et al. "I-fit enkulu ye-fit scoit stnl7.1 edibanisa i-protein ye-viedogenis enkulu ehambelana nobude bentamo yeziqhamo." Ijenali yeBiotechnology 18.7 (2020).
