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I-Bmkmani S3000_SPEATAIAL

I-Bmkma -u S3000_SPEATAIAL STRAPTOME iboniswe umfanekiso

I-Spatial Tracttoptophestotophestophetortotortotoyisi ibala ngaphambili kokuveliswa kwesayensi, ukuxhobisa abaphandi ukuba baphathe kwiipatheni ze-biricate kwi-tisteries ngexesha lokugcina imeko yabo yendawo. Ngamafutshane amaqonga ahlukeneyo, i-Bmkgene iphuhlise i-BMKMKMEA S3000 I-SPRCIPTOME Chip, Ukuqhayisa isisombululo esiphuculweyo se-3.,5μm, ukufikelela kwiindawo ezinamanqanaba emigangatho emininzi. I-S3000 ye-S3000, ibonisa malunga nezigidi ezingama-4 amabala, isebenzisa i-microwells ye-Beads ilayishwe kwindawo yokuvalwa kwendawo ye-bartied. Ithala leencwadi le-CDNA, inesidima esinebhakhowudi ye-spatial, ilungiselelwe ukusuka kwi-S3000 Chip kwaye ilandele kwiqonga le-Illina novaseq Ukudityaniswa kweesampulu ze-barcotity ze-barcodity kunyeUs kuqinisekisa ukuchaneka kunye nokuchasana kwedatha evelisiweyo. I-BMKMKMMU S3000 ye-chip iguqulela kakhulu, inika useto lokusombulula amanqanaba amaninzi anokutsalwa ngokufanelekileyo kwizicubu ezahlukeneyo kunye namanqanaba afunekayo enkcukacha. Olu tshintsho lubekho kwi-chip njengolunyulo olubalaseleyo lwezifundo ze-spatial zendawo ezahlukeneyo, qinisekisa ukuba i-qubste ye-spatial spatial ngengxolo encinci. Ukusetyenziswa kwetekhnoloji yokuthengisa i-Bmkmanu S3000 kwenza ukuba ukupheliswa kwedatha yoRhwebo kwimida yeeseli, okukhokelela kuhlalutyo olunentsingiselo yebhayoloji. Ngaphaya koko, isisombululo esiphuculweyo se-S3000 se-S3000 kwinani eliphezulu lezifo zemfuza kunye ne-UMAS efunyenweyo kwiseli nganye, enika uhlalutyo oluchanekileyo ngakumbi lweepateni zokukhutshelwa kwendawo kunye nokudityaniswa kweeseli.

Qhagamshelana nathi

Iinkcukacha zenkonzo

I-Bioinformatics

Iziphumo

Umahluko phakathi kwe-S3000 kunye ne-S1000

I-Bmkma -u S3000 SPATAIL SPATIPTOM

Iimbonakalo

-Sigqibo: 3.5 μm

-Iidayam: 2.5 μm

-Inani lamabala: malunga nezigidi ezingama-4

Iifomathi ezi-3 ezinokubakho: 6.8 mm * 6.8 mm, i-11 mm * i-11 mm okanye i-15 mm * 20 mm

-Ibhedi nganye ye-barcode ilayishwe ngamacandelo ama-4:

• I-Poly (DT) Umsila we-MRNA Priing kunye neCDNA Synthesis,

• Isikhombisi esikhethekileyo semolekyuli (i-UMI) ukulungisa i-ames bias

• ibhakhowudi yendawo

• Ukulandelelana kwenxalenye yokufunda inxenye enye

- h & e kunye ne-fluorescent yamacandelo

-Ukusebenza kokusebenzisa itekhnoloji yecandelo leseli: Ukudityaniswa kwe-H & E Stainses, i-Fluorescent Staineng, kunye ne-Fluoresting, kunye ne-RNA ye-RNA ukumisela imida yeseli nganye kwaye ibonelele ngokuchanekileyo isisele se-FA kwaye ngokuchanekileyo inike intetho yesifo sentloko kwaye sinike amagama ngokuchanekileyo kwiseli nganye. Ukulungiswa kwe-Dowream yendawo yendawo yendawo esekwe kwi-bin yeseli.

-Ukunokwenzeka ukuze ufezekise uhlalutyo lwesimo se-stolutile: Uhlalutyo lwenqanaba lamanqanaba amaninzi ukusuka kwi-100um ukuya kwi-3.5m ukusombulula amanqaku ahlukeneyo kwizicubu kwisisombululo esiphezulu.

Izibonelelo zeBmkmanu S3000

-Ukuphindaphinda Ukuthinjwa amabala ukuya kwizigidi ezingama-4: Ngesisombululo esiphuculweyo se-3.5 m, esikhokelela kwi-gene kunye ne-UMI kwiseli nganye. Oku kuphumela ekuphuculeni ubuhlanga obuphuculweyo kweeseli ezisekwe kwiiprofayili ze-Shorcricks, ngenkcukacha ezilungileyo ezihambelana nolwakhiwo lwezicubu.

-Isisombululo seselula:Indawo nganye yokufaka iqulethwe> Izigidi ezi-2 zebhakhowudi ezinobubanzi be-2,5 μm kunye nesithuba se-5 μm phakathi kwendawo i-stoctriptome i-ssorction yesisombululo seselula (5 μm).

-Uhlalutyo lwamanqanaba amaninzi:Uhlalutyo lwenqanaba eliguqulweyo olususela kwi-100 μm ukuya kwi-5 μm ukusombulula amanqaku ahlukeneyo kwizilonda kwisigqibo esigqibeleleyo.

-Inokwenzeka ukuba isebenzise "ezintathu kwi-Slide" yeTekhnoloji yeTekhnoloji:Ukudibanisa i-fluorescence staices, i-H & E Suing, kunye ne-RNA ye-RNA i-Algorithm ye-Algorithm yethu "enesibini" ixhobisa imida yemida yefowuni elandelayo.

-Ihambelana neqonga lamaqonga amaninzi: Zombini ii-NG kunye nokulandelelana okude okufunwayo.

-Uyilo oluguqukayo lwendawo ye-1-8 esebenzayoUbungakanani bendawo yokufaka idatha iyaguquguquka, kunokwenzeka ukuba usebenzise iifomathi ezi-3 (6.8 mm * 6.8 mm * i-11 mm kwaye i-20 mm * 20 mm * 20 mm)

-Inkonzo yokumisa enye: Dibanisa onke amava kunye namanyathelo asekwe kwizakhono, kubandakanya ukuvelisa i-Cornyo, ulungiselelo lwezinto, kwi-tissue, i-spacial barcing, ukulungiselela iilayibrari, kunye ne-bioinformatics.

-I-bioinformatics ebanzi kunye nokuboniswa komfanekiso onobuhlobo:Iphakheji iquka ii-30 zohlalutyo kunye namanani angama-100, amanani aphezulu, adityaniswe nokusetyenziswa kwe-InORouse Apple ukuze ibe nomfanekiso-ngqondweni weseli kunye nendawo yokuqhekeka kweseli.

-Uhlalutyo lwedatha ngokweenkcukacha kunye nokubonwa: Iyafumaneka kwizicelo ezahlukeneyo zophando

-Iqela lezobuchwephesha kakhulu: Ngamava kwiindidi ezingama-250 zeetishu ezingama-250 kunye neentlobo ezili-100 ze-100 + kubandakanya umntu, imouse, inyamakazi, intlanzi, intlanzi kunye nezityalo.

-Uhlaziyo lwexesha lokwenyani kwiprojekthi yonke: Ulawulo olupheleleyo lwenkqubela phambili.

-Uhlalutyo oludibeneyo oluhlelelekileyo kunye neseli-call-call

Inkcazo yenkonzo

Iifoto zeSampula

Ithala leencwadi

Isicwangciso sokulandelelana

Idatha eyacetyiswayo

Ulawulo lwemeko

Iisampulu ze-OCT

(Ububanzi be-EPOGETTER: App.

I-6 × 6 × 6 mm³)

Iibhloko ezi-2 kwisampulu

I-1 yokuvavanywa, 1 ye-backup

Ithala leencwadi le-S3000

I-Mulmina Pe150

I-160k Pe ifundwe nge-100 ye-250 GB)

I-RIN> 7

Ngeenkcukacha ezithe kratya kwiSikhokelo sokuLungiselela isampulu yesampulu kunye nokuHlala kweNkonzo, nceda uzive ukhululekile ukuthetha neIngcali ye-bmkgene

Ukuhamba kwenkonzo

Kwisigaba sokulungiselela isampula, isilingo sokukhupha iRok senziwa ukuqinisekisa ukuqinisekisa ukuba i-RNA yomgangatho ophezulu inokufunyanwa. Kwinqanaba lokuphucula i-tishu yeziculi amacandelo aqingqiweyo kwaye abekelwe kwaye iimeko ze-pergaabailis ze-Mnumzana Ukukhutshwa kwezicubu zenzekile. Iprotocol efanelekileyo ke emva koko isetyenziswa emva koko isetyenziswa emva koko i-protogral ye-ofisi efanelekileyo emva koko isetyenziswa ke ngexesha lokwakhiwa kwelayibrari, kulandele ukulandelelana kunye nohlalutyo lwedatha.

Imowudi yokusebenza epheleleyo yenkonzo iquka uhlaziyo lwexesha lokwenyani kunye noqinisekiso lwabathengi ukugcina i-loop yokuphendula iphendulayo, iqinisekisa ukubulawa kweprojekthi.

Ngaphambili: IIMPAPASHO ZEPHONZO ZE-DNBSEQ

Okulandelayo:

Idatha eveliswe yi-Bmkmanu S3000 ihlalutya isebenzisa isoftware "ye-BSTMTRIX Ukusuka apho, ingxelo esemgangathweni ivelisiwe equka ulawulo lwedatha, uhlalutyo lwangaphakathi lwesampulu kunye nohlalutyo lweqela.

- Ulawulo loMgangatho weDatha:

-Imveliso yemveliso kunye nenqaku elisemgangathweni

-Ukuvangelwa yiyo kwindawo nganye

-Iicusi

-Uhlalutyo lwangaphakathi lwesampulu:

- Ubutyebi begxa

-Iindawo ezihlanganisiweyo, kubandakanywa ukuncitshiswa kohlaziyo

-Uhlalutyo lwamabala ahlukeneyo phakathi kwamaqela: Ukuchongwa kwe-genes yemakisha

-Sebenzisa isichazi kunye nokuphucula ubume bemakisha

- Uhlalutyo lweqela

-Ukudityaniswa kwakhona kwamabala ukusuka kuzo zombini iisampulu (umz.. Njani kwaye ulawulo) kunye neqela kwakhona

-Ukuchongwa kwemfuza yamamark yeqela ngalinye

-Sebenzisa isichazi kunye nokuphucula ubume bemakisha

-Imbonakalo eyahlukileyo yeqela elinye phakathi kwamaqela

Ukongeza, i-Bmkgene iphuhlise i-BMKGEGEE "BSTVENIX" sisiXhobo esiNcinci esenza ukuba umsebenzisi enze ukuba umsebenzisi akwazi ukubona intetho yesisu kunye nendawo yendawo kwizisombululo ezahlukeneyo.

I-Bmkgene ibonelela ngeenkonzo zendawo yendawo kwi-SOCCIF ichaze isisombululo seseli enye (esekwe kwi-bin ye-bin okanye kwi-bilevel yesikwere kwi-100um ukuya kwi-3.5m).

Idatha yeProfati yendawo evela kumacandelo e-tissue kwisilayidi se-S3000 esenziwe kakuhle nangezantsi.

Imeko yokufunda 1: Ingqondo ye-mouse

Uhlalutyo lwecandelo lobuchopho olune-S3000 lubangele ukuchongwa kweeseli ezingama-94 000, kunye nokulandelelana kwe-~ 2000 kwiseli nganye. Isisombululo esiphuculweyo se-3.5 umphumo kuhlanganiso olucacileyo lweeseli ngokusekwe kwiipatheni zendlela yokukhuphela, kunye namaqela eeseli ezibonisa izakhiwo ezahlukileyo zobuchopho. Oku kujongwa ngokulula ngokubona ukuhanjiswa kweeseli ezidityanisiweyo njengo-Oligedeendrocyte kunye neeseli ezincinci eziphantse zibekho ngombala omhlophe nowamhlophe, ngokulandelelana.

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Imeko yesifundo 2: Imibungu yemouse

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Uhlalutyo lwecandelo lombungu wemouse nge-S3000 lubangele ukuba kuchongwe iiseli ezingama-2200 000, kunye nokulandelelana kwe-1600 yeemfumba nganye. Isisombululo esiphuculweyo se-3.5m sakhokelela kwinkcazelo eneenkcukacha ezininzi zeeseli ezisekwe kwiipatheni zendlela yokugcina, ezinamaqela ali-12 kwindawo yeliso kunye ne-28 ezinamaqela kwingqondo.

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