INdibano ye-Bactory ye-Bactery
Sinika inkonzo yeNdibano ye-Bactery egcweli epheleleyo, qinisekisa izithuba ze-0. Oku kunokwenzeka ngokudibanisa itekhnoloji yokufunda ixesha elide, njenge-nanopore kunye ne-pacbio yendibano kunye nokufunda ngokufutshane kunye ne-Ilroumina yokungqinisisa i-Ints Ukuqinisekiswa kwe-ont kufunda. Inkonzo yethu ibonelela nge-biinorifolomu ye-biorionacic epheleleyo kwiNdibano, i-ANSINALS, NOKUGQIBELA WOKUGQIBELA Le nkonzo inika ukuphuculwa kophuhliso lweendawo zokubhengeza ezichanekileyo zeZifundo ezizodwa zemfuza kunye ne-NEIMIC. Ukongeza, yindlela isiseko sezicelo ezinje ngokulandelelana koxinzelelo, ubunjineli bemvelo, kunye nophuhliso lwetekhnoloji ye-genofial, qinisekisa ukuba idatha ethembekileyo neyasimahla ye-genomic genocy ebonakalayo ebaluleke kakhulu ekuqhubeleni phambili ukuqonda kwesayensi kunye ne-bietechnologran.
Iimpawu zenkonzo
● Ngokukhetha ezimbini ezinokubakho ukukhetha ukuxhomekeka kwinqanaba elifunayo lokupheliswa kwe-genome.
● Uyilo lwe-Genome Inketho: Funda ukulandelelana okufutshane ne-Inlina Novaseq Pe150.
● Gqibezela i-Genome eyi-0.
● Ukufundwa ixesha elide kwi-Nanopore Promethion 48 okanye i-Pacbio Revio yeNdibano yeGenome.
● Ukufundela ukulandelelana kwe-Ilnumina Novaq yenkqubo yokuqinisekiswa kwe-genome kunye nempazamo yokulungisa (i-nanopore) okanye ukuvelisa idrame ye-genome.
Izibonelelo zeNkonzo
●I-zero-genome iqinisekisiweOku kungenxa yokudityaniswa kokulandelelana kwe-Illina ngokulandelelana okufundekayo (i-nanopore okanye i-pacbio).
●Gcwalisa i-bioinfowOku kubandakanya indibano ye-genome kunye nokuxelwa kwangaphambili kwezinto ezininzi ze-gemic, isichazi-magama e-genese, kunye nokuboniswa kwe-genome, kunye nemiboniso ye-genome efana ne-chicos yenqe.
●Ubuchule obubanzi: Ngaphezulu kwe-genomes engaphezulu kwama-20,000 i-genomes ehlanganisene, i-Bmkgene izisa ngaphezulu kweshumi lamava, iqela lohlalutyo olunobuchule, umxholo obanzi, kunye nenkxaso ebalaseleyo yokuthengisa.
●Inkxaso yasemva kwentengiso:Ukuzibophelela kwethu kugqitha ngaphaya kokugqitywa kweprojekthi kunye nexesha lenkonzo yenkonzo yasemva kwenkonzo emva kwenkonzo. Ngeli xesha, sinikezela ngeprojekthi yokulandela iprojekthi, uncedo lokucombulula iingxaki, kunye ne-Q & Iiseshoni zokujongana nayo nayiphi na imibuzo enxulumene neziphumo.
●Izicwangciso ezininzi zokulandelelana ezikhoyo:Kwiinjongo zophando ezahlukeneyo kunye neemfuno zokugqibelela kwe-genome.
Inkcazo yenkonzo
| Inkonzo | Isicwangciso sokulandelelana |
| Uyilo lwe-genome | I-Mulina Pe150 100x |
| I-0 genome | I-Nanopore 100x + i-Illina Pe150 100x Or I-Pacbio Hii 30x + i-Illina Pe150 100x (ngokuzithandela) |
Iifoto zeSampula:
| Uxinzelelo (nge / μl) | Inani lilonke (μg) | Umthamo (μl) | Od260 / 280 | I-OD260 / 230 | |
| I-pacbio | ≥20 | ≥1.2 | ≥20 | 1.7-12 | ≥1.0 |
| Nanopore | ≥40 | ≥2 | ≥20 | 1.7-12 | 1.0-3.0 |
| I-Illina | ≥1 | ≥0.06 | ≥20 | - | - |
I-Teacteria: ≥3.5x1010 iiseli
Ukuhamba kwenkonzo
Ukuhanjiswa kwesampula
Ulwakhiwo lwethala leencwadi
Ukulandelelana
Uhlalutyo lwedatha
Iinkonzo zentengiso emva
Kubandakanya nohlalutyo olulandelayo:
● Ukulandelelanisa umgangatho wedatha
● Indibano ye-genome
● Uhlalutyo lwecandelo le-genome, uqikelelo lweeCDs kunye nezinto ezininzi ze-gemic
● Functional annotation with multiple general databases (GO, KEGG, etc) and advanced databases (CARD, VFDB, etc)
● Umbono we-genome
Sinikezela nge-genome kwifomathi ye-IFARY yeMali efikelelekayo kunye nefayile ye-genome (gff).
Isichazi-mveliso-hamba
Isichazi-mali-carbohydrate
Ukuboniswa kwe-Genome- i-circos yeyelenso
Jonga i-veras iququzeleleke ziinkonzo zeNdibano ye-BMKGGENEne ngengqokelela yeempapasho eziqinisekisiweyo.
Dai, w. et al. .Ijenali yeHlabathi yeNzululwazi yezeNdalo, 24 (19), iphe. I-14669. Doi: 10.3390 / IJMS2419146666 / s1.
I-Kang, Q. Et Al. .I-zoology yeZoology, 16 (6), iphe. 798-809. Doi: 10.1111 / 1749-4877.12510.
Wang, tt et al. .Ijenali ye-Biotechnology, 260, iphe. 38-41. Doi: 10.1016 / J.jbiotec.2017.09.001.
Wang, X. ET Al. .Imida kwi-microbiology, 12, P. 754931. Doi: 10.3389 / FEMB.2021.754931 / i-bibtex.
