16S/18S/ITS Amplicon Sequencing-NGS
Iimpawu zeNkonzo
● Iqonga lokulandelelana: Illumina NovaSeq.
● Ukwandiswa kwemimandla emifutshane ye-16S, 18S kunye ne-ITS, phakathi kwezinye iithagethi zokwandisa.
● Ukhetho oluguquguqukayo lweamplicon.
● Amava eprojekthi yangaphambili aneethagethi zolwandiso ezininzi.
Iinkonzo eziluncedo
●Akunakwahlukaniswa:Ukuchongwa okukhawulezayo kokubunjwa kwe-microbial kwiisampulu zokusingqongileyo.
●Isisombululo esiphezulu: Kwizinto eziphantsi-zininzi kwiisampulu zokusingqongileyo.
●Isetyenziswa ngokubanzi: Izifundo zoluntu ezahlukeneyo ze-microbial.
●Uhlalutyo oluBanzi lwe-Bioinformatic: Iphakheji ye-QIIME2 yamva nje (ingqiqo yobungakanani kwi-microbial ecology) enohlalutyo olwahlukeneyo ngokwesiseko sedatha, inkcazo, i-OTU/ASV.
●UbuGcisa obuBanzi: Ngamawaka angama-150 eeprojekthi zokulandelelana kwe-amplicon eziqhutywa minyaka le, i-BMKGENE izisa ngaphezulu kweminyaka elishumi yamava, iqela lokuhlalutya abanobuchule obuphezulu, umxholo obanzi, kunye nenkxaso egqwesileyo emva kokuthengisa.
IiNgcaciso zeNkonzo
| Ithala leencwadi | Isicwangciso solandelelwano | Idatha iyacetyiswa |
| Amplicon | Illumina PE250 | 50/100/300K iithegi (Funda izibini) |
IiMfuno zeNkonzo
| Ugxininiso (ng/µL) | Iyonke imali (ng) | Umthamo (µL) |
| ≥1 | ≥200 | ≥20 |
● Umhlaba / udaka: 1-2g
● Umxholo wamathumbu-isilwanyana: 0.5-2g
● Iziqulatho zamathumbu-insect: 0.1-0.25g
● Umphezulu wezityalo (intlenga etyetyisiweyo): 0.1-0.5g
● Umhluzi wokubila utyetyiswe intlenga): 0.1-0.5g
● Ilindle (izilwanyana ezikhulu): 0.5-2g
● Indle (imouse): 3-5grains
● I-pulmonary alveolar lavage fluid: iphepha lokucoca
● I-swab ye-vaginal: i-5-6 swabs
● Ulusu/iswabhu yelungu lobuni/amathe/isihlunu esithambileyo somlomo/iswab yepharyngeal/i-rectal swab: 2-3 swabs
● Ii-microorganisms ezingaphezulu: iphepha lokucoca
● Umzimba wamanzi/umoya/i-biofilm: iphepha lokucoca
● I-Endophytes: 1-2g
● I-Dental Plaque: 0.5-1g
Ukuhamba komsebenzi weNkonzo
Ukuhanjiswa kwesampuli
Ukwakhiwa kwethala leencwadi
Ukulandelelanisa
Uhlalutyo lwedatha
Iinkonzo emva kokuthengisa
Ibandakanya uhlalutyo olulandelayo:
- Ulawulo lomgangatho wedatha ekrwada
- Ukuhlanganiswa kwe-OTU / i-De-noise (ASV)
- Inkcazo ye-OTU
- Uhlalutyo lweyantlukwano yeAlpha: izalathisi ezininzi, kubandakanya iShannon, iSimpson kunye neACE.
- Uhlalutyo lokwahluka kweBeta
- Uhlalutyo lwamaqela
- Uhlalutyo lonxulumano: phakathi kwemiba yokusingqongileyo kunye nokwakheka kwe-OUT kunye neyantlukwano
- I-16S ingqikelelo yemfuza esebenzayo
I-Histogram yokusasazwa kwe-taxonomic
taxonomic ubuninzi obudibanisa imephu yobushushu
Uhlalutyo lokwahluka kwe-Alpha: ijika elinqabileyo
Uhlalutyo lokwahluka kwe-beta: NMDS
Uhlalutyo lwe-Intergroup: Ukufunyanwa kwe-biomarker ye-LEFSE
Phonononga inkqubela phambili eququzelelwa yi-BMKGene ye-amplicon yolandelelwano lweenkonzo kunye ne-Illumina ngengqokelela egciniweyo yopapasho.
Dong, C. et al. (2022) 'Indibano, i-Core Microbiota, kunye noMsebenzi we-Rhizosphere Soil kunye ne-Bark Microbiota e-Eucommia ulmoides', i-Frontiers kwi-Microbiology, i-13. doi: 10.3389 / FMICB.2022.855317 / FULL.
Li, Y. et al. (2023) 'I-synthetic bacterial consortia transplantation yonyango lwe-Gardnerella vaginalis-induced bacterial vaginosis kwiigundane', i-Microbiome, i-11 (1), iphe. 1-14. doi: 10.1186/s40168-023-01497-y
Yang, J., Fu, Y. kunye noLiu, H. (2022) 'I-Microbiomes yothuli lomoya oluqokelelwe ngexesha lovavanyo lwenkxaso ye-bioregenerative esekelwe phantsi komhlaba "Lunar Palace 365"', i-Environmental Microbiomes, 17 (1), iphe. 1–20. doi: 10.1186/S40793-022-00399-0/AMAFIGURES/8.
Yin, S. et al. (2022) 'Ubuninzi obuxhomekeke kwi-Feedstock-dependent of functional genes ehambelana nokuguqulwa kwe-nitrogen elawulwa ukulahleka kwe-nitrogen ekuhlanganiseni', i-Bioresource Technology, i-361, iphe. 127678. doi: 10.1016/J.BIORTECH.2022.127678.
