Whole genome bisulfite sequencing(WGBS)
This technique, based on the bisulfite treatment of DNA to induce the conversion of unmethylated cytosines to uracil (C to U), while leaving methylated cytosines unchanged, stands as the gold-standard methodology for in-depth exploration of DNA methylation, specifically the fifth position in cytosine (5-mC), a pivotal regulator of gene expression and cellular activity.
This technique offers single-base resolution, allowing researchers to comprehensively investigate the methylome and uncover abnormal methylation patterns within the samples. By employing WGBS, scientists can gain unparalleled insights into genome-wide methylation landscapes, providing a nuanced understanding of the epigenetic mechanisms that underlie diverse biological processes and diseases.
Service Features
● Sequencing on Illumina NovaSeq.
● Requires a reference genome.
● Lambda DNA is added to monitor bisulfite conversion efficiency.
Service Advantages
● Gold Standard for DNA Methylation Research: This mature technology has high accuracy and good reproducibility.
● Wide Coverage and Single-base Resolution: The detection of methylation sites is at a genome-wide level.
● Complete Platform: We provide one-stop service from sample processing, library construction, sequencing to bioinformatics analysis.
● Extensive Expertise: We have a broad spectrum of projects completed across a diverse range of species. BMKGENE brings over a decade of experience, a highly skilled analysis team, comprehensive content, and excellent post-sales support.
● Possibility to Join with Transcriptomics Analysis: Allowing for the integrated analysis of WGBS with other omics data such as RNA-seq.
Sample Specifications
|
Library |
Sequencing Strategy |
Recommended data output |
Quality control |
|
Bisulfite treated |
Illumina PE150 |
30x depth |
Q30 ≥ 85% Bisulfite conversion > 99% |
Sample Requirements
|
Concentration (ng/µL) |
Total amount (µg) |
Additional requirements |
|
|
Genomic DNA |
≥ 5 |
≥ 400 ng |
Limited degradation or contamination |
Service Work Flow
Sample delivery
DNA extraction
Library construction
Sequencing
Data analysis
Data delivery
The service includes the following analysis:
● Raw sequencing quality control;
● Mapping to reference genome;
● Detection of 5mC methylated bases;
● Analysis of methylation distribution and annotation;
● Analysis of Differentially Methylated Regions (DMRs);
● Functional annotation of genes associated to DMRs.
5mC methylation detection: types of methylated sites
Methylation map. 5mC methylation genome-wide distribution
Annotation of highly methylated regions
Differentially Methylated Regions: associated genes
Differentially Methylated Regions: annotation of associated genes (Gene Ontology)
Explore the research advancements facilitated by BMKGene’s whole genome bisulfite sequencing services through a curated collection of publications.
Fan, Y. et al. (2020) ‘Analysis of DNA methylation profiles during sheep skeletal muscle development using whole-genome bisulfite sequencing’, BMC Genomics, 21(1), pp. 1–15. doi: 10.1186/S12864-020-6751-5.
Zhao, X. et al. (2022) ‘Novel deoxyribonucleic acid methylation perturbations in workers exposed to vinyl chloride’, Toxicology and Industrial Health, 38(7), pp. 377–388. doi: 10.1177/07482337221098600
Zuo, J. et al. (2020) ‘Relationships between genome methylation, levels of non-coding RNAs, mRNAs and metabolites in ripening tomato fruit’, The Plant Journal, 103(3), pp. 980–994. doi: 10.1111/TPJ.14778.
