TGuide Smart DNA Purification Kit
Nomoro ea lethathamo / sephutheloana
| Katse. che | ID | No. ea preps |
| 4993550(khatriji) | DP642-DE | 48 |
Tataiso ea khetho ea reagent bakeng sa TGuide S16
| Asiti ea Nuclei | Mofuta oa Mohlala | Lebitso la Sehlahiswa | Boholo ba ho paka (preps) Cartridge/ poleiti | Nomoro ea katse. Cartridge/ poleiti |
| DNA | Sehlahisoa sa Liphoofolo | TGuide Smart Magnetic Tissue DNA Kit | 48/96 | 4993547/4995038 |
| DNA | Semela & Peō | TGuide Smart Magnetic Plant DNA Kit | 48 | 4993548 |
| DNA | Mobu/Sentle | TGuide Smart Soil /Stool DNA Kit | 48 | 4993549 |
| DNA | Lihlahisoa tsa Gel & PCR | TGuide Smart DNA Purification Kit | 48 | 4993550 |
| DNA | Madi Kaofela | TGuide Smart Blood Genomic DNA Kit | 48/96 | 4993703/4995206 |
| DNA | Cell/Swab/Dry Spots, joalo-joalo | TGuide Smart Universal DNA Kit | 48/96 | 4993704/4995040 |
| RNA | Mali/Seleng/Mahlapa | TGuide Smart Mali/Cell/Tissue RNA Kit | 48/96 | 4993551/4995043 |
| RNA | Semela & Peō | TGuide Smart Magnetic Plant RNA Kit | 48 | 4993552 |
| DNA/RNA | Mekelikeli ea Mahala ea Lisele (Serum, joalo-joalo) | TGuide Smart Viral DNA/RNA Kit | 48/96 | 4993702/4995207 |
Kenyelletso ea sehlahisoa
E khutlisa DNA kapele le ka nepo ho tsoa ho gel ea TAE/TBE le sehlahisoa sa PCR.
Lethathamo la boholo ba sekhechana sa DNA: 100 bp-15 kb.
DNA e fumanoeng ka ho sebelisa kit ena e ka sebelisoa bakeng sa tšilo ea lijo, PCR, tatellano, tlhahlobo ea laeborari, ligating, liphetoho le liteko tse ling.
Likaroloana
Ho bonolo haholo ho e sebelisa
Ha ho na mosebetsi o eketsehileng oa ho penta.
Bolumo ea elution e ka fetoloa kamoo ho hlokahalang.
Ho kenya le ho sebetsa ho hloka koetliso e fokolang. Ka mananeo a kentsoeng pele, bula cartridge, khetha protocol 'me u tsamaise liteko tsa hau. Mananeo a se a loketse ho sebelisoa, hape a ka etsoa ka mokhoa o ikhethileng.
Li-reagents tse kentsoeng esale pele le lisebelisoa tse ka sebelisoang tse tsamaisanang.
Ho sebetsana le lik'hemik'hale ho felisoa ka tekanyo e kholo ka ho fetisisa ho netefatsa ts'ireletso ea ts'ebetso.
Sephetho sa Demo
300 bp segment ea agarose gel DNA tlhoekiso le ho hlaphoheloa
Khatiso ea gel ea Agarose: 1.5% (TBE)
Loading
bophahamo: 8 μl
Letšoao: 50 bp DNA lere, TIANGEN
S16-01, S16-02, le S16-03 li emela liteko tse tšoanang tsa ho hloekisa le ho hlaphoheloa ho sebelisoa 3 TGuide S16 Nucleic Acid Extractors le TGuide Smart DNA Purification Kit (4993550) ka nako e le 'ngoe.
K'hamphani: sesebelisoa sa ho hula li-spin-column tsa mofuta o tsebahalang
2000 bp segment ea agarose gel DNA tlhoekiso le ho hlaphoheloa
Khatiso ea gel ea Agarose: 1.5% (TBE)
Bophahamo ba ho kenya: 8 μl
Letšoao: D2000, TIANGEN
S16-01, S16-02, le S16-03 li emela mohlala o tšoanang oa tlhoekiso le ho hlaphoheloa.
teko e sebelisang 3 TGuide S16 Nucleic Acid Extractors e nang le TGuide Smart DNA Purification Kit (4993550) ka nako e le 'ngoe.
K'hamphani: sesebelisoa sa ho hula li-spin-column tsa mofuta o tsebahalang
Sephetho sa liteko: ho sebelisa TGuide S16 Nucleic Acid Extractor e nang le kit e ikhethileng TGuide Smart DNA Purification Kit (4993550) e ka fihlela automation e felletseng ea ho hlaphoheloa ha gel. Tšebelisano ea ho hloekisa le ho hlaphoheloa e ntle haholo, 'me sephetho sa ho ntša se molemo ho feta sa lihlahisoa tse hlōlisanang tsa lik'hamphani tse ling.
15000 bp karolo ea agarose gel DNA ho hlaphoheloa le ho hloekisa sehlahisoa sa PCR
Khatiso ea gel ea Agarose :1%(TBE)
Bophahamo ba ho kenya: 3 μl
Lesupa:
D15000,TIANGEN
G1, G2: ho hlaphoheloa ha gel ea agarose
C1: tlhoekiso ea lihlahisoa tsa PCR.
Protocol ea Manual: bukana ea "spin-column extraction kit" ea mofuta o tsebahalang oa B
Khampani: mokhoa oa magnetic bead -based extraction kit ea mofuta o tsebahalang
Sephetho sa liteko: ho sebelisa TGuide S16 Nucleic Acid Extractor e nang le kit TGuide Smart DNA Purification Kit (4993550), pholiso ea gel ea agarose hammoho le tlhoekiso ea sehlahisoa sa PCR e ka finyelloa ka bo eona, 'me sephetho sa tlhoekiso se bapisoa le sa spin-column. tlhoekiso e thehiloeng.
