Tguide S16 Nucleic Acid Extractor
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Tguide S16 Nucleic acid e ka ba teng ho isa ho li-sampole tse 16 ka nako e le 'ngoe ka lithupa tsa makernetic Ka ho sebelisa moo ho kopanngoeng hoa mokokotlo o tšoanang, sehlahisoa sena se ka itlhoekisa DNA / PRANTIZE, BACTEG
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Likaroloana
Ho sebelisana ho ba bonolo ho feta
Ha ho na mosebetsi o mongata oa pipetting. Mobuo oa ho itlotsa o ka fetoloa ha ho hlokahala.
Ho kenya le ho sebetsa ho hloka koetliso e nyane. Ka mananeo a kentsoeng pele a ne a sa kenngoa kankere, khetha protocol ebe u matha teko ea hau. Mananeo a se a ikemiselitse ho a sebelisa, hape a le bontsi.
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Mokhoa o mocha oa tlamo
Molatsoana oa makenete o amohela mokhoa o matla oa ho tlala.
Litema tsa makenete li tlama molapeng, tse netefatsang hore bothata bo ntse bo ka koahela lieta tsohle tsa masene le ha bophahamo ba kahlolo bo le nyane. Moralo o matlafatsa tlhahiso ea nyutlelie ea nyleoa.
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Moralo o nepahetseng oa tanka ea ho futhumatsa
Ho na le khoele e kentsoeng lipakeng tsa li-lysis Buffer le kholomo ea kaute e ka atlehang ho seva ka lebaka la ho seva. Ka hona, molumo oa ho hlaphoha ha tharollo ea nyutledic acid e nepahetse haholo.
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Ts'ireletso ea taolo le ts'ireletso ea operator
E ka beoa bekeng e hloekileng kapa ka har'a hood ea lik'hemik'hale.
Sesebelisoa se sebetsa ka tsela e ikemetseng le e koetsoeng.
Ts'ebetso ea thibelo ea tšilafalo e emela mekhoa e 'meli: tsamaiso ea sistimi ea kahare ea sefapano e sebetsang le tsamaiso ea sistimi ea ultraviolet, ho qoba tšilafalo ea sefapano le sampole.
Re jarollotsoe li-rerongts le tse tsamaisoang habonolo.
Ho ts'oaroa ha lik'hemik'hale ho felisoa haholo ho feta ho netefatsa polokeho ea ts'ebetso.
Liphetho tsa demo
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Sephetho
Ho tlosa DNA ka boeona ea li-sampole tse ngata ho kenyelletsa lisele, lisele, mali, lactobaclus, lactobaclus, e.coli, ho fumana chai e ntle le bohloeki.
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Od260 / od280 ho pota 1.7 ~ 1.9, le
Od260 / od230> 1.6.
Buka ea Eletion: 100 μl
Agarose Gel mahloriso: 1.5%
Ho kenya molumo: 1 μl
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U sebelisa TGIIID S16 Ha Agnal Smart Vairal Gna / Rna Kit (4993702)
Ho tlosa DNA / RNA Ho tloha mehloling e mengata ho kenyelletsa mali a mangata, swab le plasma le plasma le bohloeki bo boholo. -
Ad260 / od280 e haufi le 1.7 ~ 1.9, le od260 / od230> 1.6.
Khamphani ea k'hamphani & B: Li-Brand tse tsebahalang hantle.
ND: Lefu la Newcastle, H5: Flup ea Avian
Sensitigraph e bonyane e ka fihla likopi tse 100 / ML.
Phello: Ho sebelisa Tguide S16 ka ho hlasimolla mofuta oa mali le bohloeki bo phahameng, bo amanang le lihlahisoa tse ling tse tsebahalang.
A od260 / od280 e haufi le 1.7 ~ 1.9, le od260 / od230> 1.7.
Mohloli oa sampole: mehlala e meraro eohle ea mali. Li-apicelites tse tharo ke liteko tsa kits bakeng sa kite ka bomong.
E pheta ka tlotla ka 'ngoe e tsoa mohloling o tšoanang oa mohlala.
Boemo ba Mohlala: Mali a Frozen
Buka ea ho kenya: 200 μl
Buka ea Eletion: 100 μl
Agarose Gel mahloriso: 1.5%
Ho kenya molumo: 1 μl
M: MARSker III, Tiangen
Khamphani ea k'hamphani & B: Li-Brand tse tsebahalang haholo
2000 BP Seagueri le ho hlaphoheloa
Agarose Gel Richtuot Control: 1.5% (TBE)
Ho kenya molumo: 8 μl
Marker: D2000, Tangen
S16-01, S16-02, 'me S16-02 e emela ho hloekisoa ha mohlala oa Parallel le Teko ea Pholoso
Khamphani: Bukana ea Bokomonisi ba Spin
ABERING 2100 e ile ea sebelisoa bakeng sa Dna ea ho tseba.
Mohloli oa Mohlala: CD-1 MIC
Boemo ba mohlala: Mali a hloekileng a bocha
Sampole ea Sampole: 200 μl
Phello: Kakaretso ea RNA e nkiloeng maling ka Tguide SC16 e nang le chai e bohlale le bohloeki bo phahameng.
E kenya molumo: 1 μl. Sebete se ile sa nkoa ka makhetlo a 5 mme a laoloa. Agarose Gel of Agarose Gel mahloriso: 1%. Electrophoresis bakeng sa 20 min ho 6 v cm;
M: Tiangen Marker Iii
1-4: pelo 5-8: sebete 9-12: Lung 13-16: Liphio
Lisampole tse peli tsa lisele tse peli tsa pele li ile tsa ntšoa ke li-spin-Culm kit kit, 'me tse peli tsa ho qetela li ile tsa ntšoa ka boeona ka tjoide s16.
Mohloli oa Mohlala: CD-1 MIC
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Phello ea teko Ho hloeka ha ho joalo, e leng Od260 / Od280 / Od260> 1.7. Qetellong, Tguide S16 e ka sebelisoa ho ntša DNA ea Gebic ho tsoa lisele tsa liphoofolo libakeng tsa liphoofolo tsa spin-Clumore.
Kakaretso ea RNA E TSOANG PELE HO TSOA FAMPS e fapaneng ea semela
Sampole Me: 100 MG
Sampole pre-kalafo: mocheso o tlase oa mocheso
Agarose Gel of AgArose Gel of Agarose: 1% (Tae)
Ho kenya molumo: 1 μl
M: MARSker III, Tiangen
1-4: Pont :-8: Popo e tsoa ho 9-12: Hop Motsosi 13-16: Li-soybeants
Lik'utene tse peli tsa pele li ne li ntšoa ke li-spin-kholom kit kit le li-sampole tse peli tsa ho qetela li ile tsa ntšoa ke Tjoe S16.
Gencan DNA Extlong ea Metso ea Koro
Sampole Me: 100 MG
Sampole pre-kalafo
Agarose Gel of AgArose Gel of Agarose: 1% (Tae)
Ho kenya molumo: 2 μl
Marker: D15000, Tangen
Khamphani: Letšoao le tsebahalang
Phello Ho feta moo, ho ba le botsitso ba makhopho a makhopho ho feta ba lihlahisoa tse ling tsa tlholisano tsa likhamphani tse ling. Tekolong ena, hoo e ka bang ka 15 μg ea nyutledic actid ho tloha ho 100 MG280 / OD260> 2.0.
