Setsi sa Khokahano ea Khokahano ea Li-Lockied Sequence (Slaf-Seq)
TLHOKOMELISO
Morero oa botekgeniki
Likarolo tsa lits'ebeletso
● Equenction ho NovaseQ e nang le Pe150.
● Ho pheha ka laebrari ka meqomo e 'meli, e nolofalletsang ea mehlala e fetang e fetang 1000.
● Mokhoa ona o ka sebelisoa kapa ntle le a ho bua ka sengoliloeng se fapaneng, le lipeipi tse fapaneng tse fapaneng bakeng sa nyeoe ka 'ngoe:
Ka genesome: snp le ho sibolla ka hare
Ntle le ho bua ka mokhoa oa ho hlophisa: SNTP SNCLTERING LE SNP Discovery
● hoKa silioPele, moralo oa tšebetso ea enzyme o hlahlojoa o hlahlojoa o hlahisang libiti tsa linoko tse tšoanang ka geneme.
● Nakong ea tlhahlobo ea pele ho tlhahlobo, motsoako o meraro o lekiloeng ka sampole tse 9 ho hlahisa libuka tse 9 tsa Slaf, 'me tlhahisoleseling ena e kopantsoe ho kopanya morero oa enzyme bakeng sa morero.
LITLHAKISO TSA BOPHELO
●Li-diaric tse phahameng tsa lefutso: Ho kopanya ts'ebetso e phahameng ea Barcode ho e lumella ka tatellano ea palo e kholo ea baahi ba bangata, 'me ho holisa linotsi tse matlafatsang lipotso tse mabapi le lipotso tsa lipatlisiso tse fapaneng.
● Ho itšepa ho tlase ho mofuta oa genemeE ka sebelisoa ho mefuta ka kapa ntle le mofuta oa litšupiso.
●Morero o feto-fetohang oa scheme: Enzyme e le 'ngoe, enzzyme e kopaneng, enzzyme TheKa silioMorero oa pele o etsoa ho netefatsa moralo o nepahetseng oa enzyme.
● Ts'ebetso e phahameng ea ho etsa liteko tsa enzymatic: Mokhoa oaKa silioMorero oa pele-pele le teko ea pele ho teko e phethahetseng le ho tsamaisa li-tag tsa slaf ho chromosome (1kb) le ho fokotsa ho pheta-pheta (<5%).
●Boitsebi bo boholoSehlopha sa rona se tlisa boiphihlelo bo boholo morerong o mong le o mong, ka ho kenyelletsa le limela tse makholo, ho kenyeletsoa limela le likokoana-hloko, linonyana.
● Ho itlhomme pele: Bmkgene e qalile ho kopanngoa ka mokhoa o kopaneng oa ho ts'epahala le ho nepahala ha tlhahiso ea ho qetela.
Litlhaloso tsa Tšebeletso
| Mofuta oa tlhahlobo | Palo e khothalletsoang | Leano le thata | |
| Botebo ba tag tag | Nomoro ea tag | ||
| Limmapa tsa liphatsa tsa lefutso | Batsoali ba 2 le> Bana ba 150 | Batsoali: 20x wgs Ho phatloha: 10x | Boholo ba Gename: <400 mb: Wgs e khothalletsoa <1GB: Li-tag tsa 100k 1-2GB ::: 200nd tags > 2GB: Li-tag tsa 300k Mags ea Max 500k |
| Lithuto tsa Bothori tse ngata (Gwas) | Sampole ea ≥200 | 10x | |
| Phepo ea lintho tsa boikoetliso | Lisa tsa sampole tsa ≥3, ka lisamona tse 10 ho tloha ho subgroup e 'ngoe le e' ngoe | 10x | |
Litlhoko tsa Tšebeletso
TLHOKOMELISO ← 1 NG / μl
Kakaretso e felletseng ≥ 80 NG
Nanodrop od260 / 280 = 1.6-2.5
Agarose gel: nor khantso kapa tšilafalo e fokolang
TLHOKOMELISO EA SMONPLE
Sejana: 2 ML Centrisuge Tube
(Bakeng sa sammopole, re khothaletsa hore re se ke ra boloka ethanol)
Samuele Labening: Mehlala e hloka ho ngolloa ka ho hlaka le ho tšoana ho fetisetsa foromo ea tlhaiso-leseling ea mohlala.
Thomello: leqhoa le omileng: Mehlala e hloka ho pateloa ka mekotleng ebe e patoa ka leqhoa.
Ts'ebeletso ea Ts'ebetso
Sampole qc
Teko ea sefofane
Teko-ea Slaf-Teko
Litokisetso tsa laebrari
E lekantsoe
Tlhahlobo ea data
Litšebeletso tsa ho rekisa ka mor'a ho rekisa
E kenyelletsa tlhahlobo e latelang:
- TLHOKOMELISO EA PUSO QC
- Nts'etsopele ea tag ea Slaf
'Mapa ho litšupiso tsa mohlala
Ntle le geness ea ho bua: ho tlatlapa
- Litlhahlobo tsa li-tag tsa Slaf.: Lipalo-palo, li ajoa ka genome
- Tšoaetso ea matšoao: SNP, indel, SNV, CV Call le mongolo
Tsamaiso ea li-tag tsa slaf ho chromosome:
Tsamaiso ea SNPS ho chromosomes:
Snop
| Selemo | Khársal | IF | Sehlooho | Likopo |
| 2022 | Phatlalatso ea tlhaho | 17.694 | Motheong oa gebiono oa giga-chrobomes le giga-giamono granime ea peony Paeonia Ostii | Slaf-Gwas |
| 2015 | Phytologist e ncha | 7.433 | Lipontšo tsa bolo ea maoto a li-anchor tsa anchor tsa Genomic tsa Bohlokoa ba Agronamic ho Soybeans | Slaf-Gwas |
| 2022 | Sekhotla sa Patlisiso e Akarelletsoang | 12.822 | Kenyelletso ea maiketsetso ea maiketsetso ea maiketsetso ea GoFSypium ea Barbades ho G. Hirsetum senola loci bakeng sa ntlafatso e le 'ngoe ea boleng ba k'hothone le chai Mekhoa ea | Li-Gef-ho Chesefe tsa Tikoloho |
| 2019 | Semela sa molecular | 10.81 | Mesebetsi ea Baofisi ea Gemiciic le Kopano ea Novo e senola tšimoloho ea Weedy Raese joalo ka papali ea ho iphetola ha lintho | Li-Gef-ho Chesefe tsa Tikoloho |
| 2019 | Genetics ea tlhaho | 31.616 | Tatellano ea geneme le phapang ea liphatsa tsa lefutso ea cyprins cypio | 'Mapa oa Seballo |
| 2014 | Genetics ea tlhaho | 25.455 | Genome ea peanut e lenngoeng e fana ka leseli ka Setsi Karyonypes, Polyploid Phetoho ea ho iphetola ha lintho le lijalo. | 'Mapa oa Seballo |
| 2022 | Semela biotechnology kopano | 9.803 | Boitsebiso ba st1 bo senola khetho e kenyelletsang ho otla ha morphology ea peo le litaba tsa oli nakong ea bophelo ba soyabean | Nts'etsopele ea Slaf-Marker |
| 2022 | Mochini oa Moruti oa Machabeng oa Molecular | 6.208 | Boitsebiso le DNA Marker Nts'etsopele ea Sesole sa Wheat-Leymus (2D) Ho senyeha ha chromosomentotion | Nts'etsopele ea Slaf-Marker |
| Selemo | Khársal | IF | Sehlooho | Likopo |
| 2023 | Fronders inflection | 6.735 | QTL Mappar le tlhahlobo ea ho fana ka litlolo tsa tsoekere nakong ea litholoana tsa pyrus pyrifalia | 'Mapa oa liphatsa tsa lefutso |
| 2022 | Semela biotechnology kopano | 8.154 | Boitsebiso ba st1 bo senola khetho e kenyelletsang ho otla ha morphology ea peo le litaba tsa oli nakong ea liphoso tsa sonya
| Snop call |
| 2022 | Fronders inflection | 6.623 | Marang-rang a kopanelang a tšosang a neng a se na phenocheng a thata tikolohong ea komello.
| Gwas |
