Nyane ea RNA ea Nkoe-Iltumina
Likaroloana
● Ho pheha ha laebrari ho kenyelletsa mohato oa khetho ea boholo
● Litlhahlobo tsa bioinforricric li sehiloe ho feta polelo ea Mirna le liphofu tsa tsona
LITLHAKISO TSA BOPHELO
●Tlhahlobo e akaretsang ea BioinForSics:Ho thusa ho khetholloa ha 'majene, ho tsebahala ha sepheo sa Mirnas, le ho ruisa ho sebetsang le ho ruisa le libaka tse ngata tsa bana (Kegg, tsamaea)
●Taolo e thata ea boleng: Re kenya taolo ea taolo ea mantlha ka mekhahlelo eohle, ho tloha ka sampole le ho pheha ha laeborari ho e lekanya le e biointoriatics. Ho beha leihlo lena la bokantle ho tiisa ho etsa hore ho be le litholoana tse feto-fetohang litholoana tse fapaneng.
●Ts'ehetso ea Morekisi: Boitlamo ba rona bo atolosa ho feta ho phetheloa ha mosebetsi ka nako ea litšebeletso tse 3. Ka nako ena, re fana ka thuso ea morero, ho nahanisisa ka thuso le linni ho sebetsana le lintlha tse amanang le liphetho.
●Boitsebi bo boholo: Ka rekoto ea pina ea ho koala ka katleho merero e mengata ea SRNA e akaretsang mefuta e fetang 300 e koahela lintho tse fapaneng tsa lipatlisiso, sehlopha sa rona se tlisa leruo morerong le leng le le leng.
Litlhoko tsa sampole le ho tsamaisoa
| Laeborari | Sethala | Lintlha tse khothalelitsoeng | Lintlha QC |
| Boholo bo khethiloeng | Ilmina Se50 | 10m-20m ea bala | Q30085% |
Litlhokahalo tsa sampole:
Nucleotives:
| Conc. (NG / μl) | Palo (μg) | Bohloeki | Bothata |
| ≥ 80 | ≥ 0.8 | Od260 / 280 = 1.7-2.5 Od260 / 230 = 0.5-2.5 E lekantsoeng kapa e se nang protheine e bontšitsoeng ka gel. | Rin≥0.0; 5.0≥28s / 18s≥ls1.0; LIMITED kapa ha ho na bophahamo ba motheo |
● Lijalo:
Motso, stem kapa petal: 450 mg
Lekhasi kapa peo: 300 mg
Litholoana: 1.2 g
● Phoofolo:
Pelo kapa ka mpeng: 450 mg
Viscera kapa bokong: 240 mg
Muscle: 600 mg
Masapo, moriri kapa letlalo: 1.5g
● Arthropods:
Likokoanyana: 9G
Crustacea: 450 mg
● Mali a mabeli: Li-Tubes tse 2
● Lisele: 106 Lisele
● Serum le Plasma:6 ml
TLHOKOMELISO EA SMONPLE
Sejana: 2 ML Centrifige Tube (Tin foil ha e khothalletsoe)
A nkatela pele: sehlopha + sa setšoantšo sa A1, A2, A3; B1, B2, B3.
Thomello:
1. Ice-ish: Mehlala e hloka ho paka ka mekotleng ebe o patoa ka leqhoa.
2. Li-tubes tse katiloeng: li-sampole tsa RNA li ka omisoa ho DNA Stabilization tjee (mohlala.
Phallo ea Service Flower
Moralo oa liteko
Ho tsamaisa Mohlala
Rna Eketso
Kaho ea laebrari
E lekantsoe
Tlhahlobo ea data
Litšebeletso tsa ho rekisa ka mor'a ho rekisa
Biolosforformatics
● Taolo ea boleng bo holimo ea data
● Classificiation ea SRNA
● Ho amohela mofuta oa litšupiso
● Tseba Mirna e tsebahalang le e seng na ha e na "
● Tlhahlobo e fapaneng le Micro ha e fapana
● Sengoloa se sebetsang sa merero ea basali ba Mirisna
Boitsebiso ba Mirna: sebopeho le botebo
Polelo e fapaneng ea Mirna - hierchical cluster
Ts'ebetso ea ts'ebetso ea sepheo sa sepheo se hlalositsoeng se sa rarollang mirnas
Plaela tsoelo-pele ea lipatlisiso e hlophiselitsoeng Litšebeletso tsa Litšebeletso tsa BMKGene 'Srna Sequens Litšebeletso tsa Litšebeletso tsa Bmkgene
Chen, H. et al. . 108038. Doi: 10.1016 / J.Plaphy.2023.108038.
Li, h. et al. . Doi: 10.15252 / Emmbr.202255037 / Suppl_file / Em-Sup-0004-Sdatafig4.tif.
Yu, j. et al. . . 5726. Doi: 10.3390 / Ijms24065726 / s1.
Zhang, M. et al. . Doi: 10.1159 / 000489649.
