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DNBSEQ lilaeborari tse entsoeng esale pele
DNBSEQ, e ntlafalitsoeng ke MGI, ke theknoloji e ncha ea NGS e khonneng ho fokotsa litšenyehelo tsa tatellano le ho eketsa sephetho. Tokiso ea lilaebrari tsa DNBSEQ e kenyelletsa ho arohana ha DNA, ho lokisoa ha ssDNA, le ho phahamisa selikalikoe ho fumana li-nanoballs tsa DNA (DNB). Ka mor'a moo, tsena li laeloa holim'a sebaka se tiileng 'me ka mor'a moo li hlophisoa ke motsoako oa Probe-Anchor Synthesis (cPAS). Theknoloji ea DNBSEQ e kopanya melemo ea ho ba le tekanyo e tlaase ea liphoso tsa amplification le ho sebelisa mekhoa ea liphoso tse phahameng ka ho fetisisa ka li-nanoballs, e leng se hlahisang tatellano e nang le phallo e phahameng le ho nepahala.
Ts'ebeletso ea rona ea tatellano ea laeborari e entsoeng esale pele e thusa bareki ho lokisa lilaeborari tsa tatellano ea Illumina ho tsoa mehloling e fapaneng (mRNA, genome eohle, amplicon, lilaeborari tsa 10x, har'a tse ling), tse fetoletsoeng ho lilaeborari tsa MGI lilaboratoring tsa rona hore li hlophisoe ho DNBSEQ-T7, e nolofalletsang. litefiso tse phahameng tsa data ka litšenyehelo tse tlase.
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Illumina lilaebrari tse entsoeng esale pele
Theknoloji ea tatellano ea Illumina, e ipapisitseng le Sequencing by Synthesis (SBS), ke popontšuoa ea lefatše ka bophara ea NGS, e ikarabellang bakeng sa ho hlahisa ho feta 90% ea data ea tatellano ea lefatše. Molao-motheo oa SBS o kenyelletsa ho nahana ka li-terminator tse fetolehang tse ngotsoeng ka fluorescent ha dNTP ka 'ngoe e eketsoa, 'me ka mor'a moo ea thijoa ho lumella ho kenyelletsoa ha setsi se latelang. Ka li-dNTP tse 'ne tse ka fetolehang tse tlamang li-terminator tse teng potolohong e' ngoe le e 'ngoe ea tatellano, tlholisano ea tlhaho e fokotsa leeme la kenyelletso. Theknoloji ena e feto-fetohang e ts'ehetsa lilaebrari tse baloang ha motho a le mong le tse peli, tse sebetsanang le mefuta e mengata ea likopo tsa genomic. Bokhoni ba tatellano e phahameng ea Illumina le ho nepahala bo e beha e le motheo oa lipatlisiso tsa genomics, e matlafatsa bo-ramahlale ba ho manolla boqhetseke ba liphatsa tsa lefutso ka lintlha tse ke keng tsa lekanngoa le bokhabane.
Ts'ebeletso ea rona ea tatellano ea laeborari e entsoeng esale pele e thusa bareki ho hlophisa lilaeborari tse tatellano ho tsoa mehloling e fapaneng (mRNA, genome eohle, amplicon, lilaeborari tsa 10x, hara tse ling). Kamora moo, lilaebrari tsena li ka romelloa litsing tsa rona tsa tatellano bakeng sa taolo ea boleng le tatellano ea li-platform tsa Illumina.
