-
Papiso ea Genomics
Papiso ea genomics e kenyelletsa tlhahlobo le papiso ea tatellano ea liphatsa tsa lefutso le libopeho har'a mefuta e fapaneng. Sebaka sena se batla ho senola ho iphetola ha lintho, ho khetholla mesebetsi ea liphatsa tsa lefutso, le ho hlakisa mekhoa ea taolo ea liphatsa tsa lefutso ka ho tsebahatsa libopeho le tatellano e bolokehileng kapa e fapaneng ho pholletsa le lintho tse fapaneng. Boithuto bo felletseng ba ho bapisa liphatsa tsa lefutso bo akaretsa litlhahlobo tse kang malapa a lefutso, nts'etsopele ea ho iphetola ha lintho, liketsahalo tsa ho pheta-pheta liphatsa tsa lefutso kaofela, le phello ea likhatello tse khethiloeng.
-
Liphatsa tsa lefutso tsa ho iphetola ha lintho
Sethala sa tlhahlobo ea liphatsa tsa lefutso tsa baahi le kholo ea ho iphetola ha lintho se thehiloe motheong oa boiphihlelo bo bongata bo bokelletsoeng ka har'a sehlopha sa BMK R&D ka lilemo. Ke sesebelisoa se bonolo sa basebelisi haholo-holo bakeng sa bafuputsi ba sa ithuteng haholo ho bioinformatics. Sethala sena se thusa tlhahlobo ea mantlha e amanang le liphatsa tsa lefutso tse amanang le ho aha lifate tsa phylogenetic, tlhahlobo ea ho se lekane ha khokahano, tlhahlobo ea mefuta-futa ea liphatsa tsa lefutso, tlhahlobo e ikhethileng, tlhahlobo ea kamano, PCA, tlhahlobo ea sebopeho sa baahi, jj.
-
Hi-C Based Genome Assembly
Hi-C ke mokhoa o etselitsoeng ho ts'oara tlhophiso ea chromosome ka ho kopanya litšebelisano tse thehiloeng ho proximity le tatellano ea maemo a holimo. Ho lumeloa hore matla a litšebelisano tsena a amana hampe le sebaka sa 'mele ho li-chromosome. Ka hona, data ea Hi-C e sebelisoa ho tataisa ho kopanya, ho hlophisa, le ho tsamaisa tatellano e kopaneng ho genome e hlophisitsoeng le ho tiisa tseo palo e itseng ea li-chromosome. Theknoloji ena e matlafatsa kopano ea boemo ba chromosome genome ha ho se na 'mapa oa liphatsa tsa lefutso o thehiloeng ho baahi. Genome e 'ngoe le e' ngoe e hloka Hi-C.
-
Semela / Phoofolo De Novo Genome Sequencing
Ho tloha Novotatellano e bolela kaho ea mofuta oohle oa lefutso ho sebelisoa mahlale a tatellano ha ho se na genome. Kenyelletso le kamohelo e pharalletseng ea tatellano ea moloko oa boraro, e nang le lipalo tse telele, e ntlafalitse haholo kopano ea genome ka ho eketsa ho fetana lipakeng tsa ho bala. Ntlafatso ena e bohlokoa haholo ha o sebetsana le liphatsa tsa lefutso tse phephetsang, tse kang tse bonts'ang heterozygosity e phahameng, karo-karolelano e phahameng ea libaka tse ipheta-phetang, li-polyploid, le libaka tse nang le likarolo tse pheta-phetoang, litaba tse sa tloaelehang tsa GC, kapa ho rarahana ho hoholo hoo hangata ho sa kopanngoeng hantle ho sebelisoa tatellano ea ho bala nako e khuts'oane. a le mong.
Tharollo ea rona ea ho emisa e le 'ngoe e fana ka lits'ebeletso tse kopaneng tsa tatellano le tlhahlobo ea bioinformatic e fanang ka genome ea boleng bo holimo e kopaneng ea de novo. Phuputso ea pele ea genome le Illumina e fana ka likhakanyo tsa boholo ba genome le ho rarahana, 'me boitsebiso bona bo sebelisetsoa ho tataisa mohato o latelang oa tatellano ea nako e telele le PacBio HiFi, e lateloa kede novokopano ea li-contigs. Tšebeliso e latelang ea kopano ea HiC e thusa ho tiisa li-contigs ho genome, ho fumana kopano ea boemo ba chromosome. Qetellong, genome e hlalosoa ka ho bolela esale pele ka liphatsa tsa lefutso le ka tatellano ea liphatsa tsa lefutso tse hlalositsoeng, ho sebelisa li-transcriptome tse nang le lipalo tse khutšoane le tse telele.
-
Sequencing ea Exome ea Motho e Felletseng
Human Whole exome sequencing (hWES) e ananelwa hohle e le mokgwa wa tatelano o baballang tjhelete le o matla bakeng sa ho qolla diphetoho tse bakang mafu. Leha e ka ba 1.7% feela ea genome kaofela, li-exons li bapala karolo ea bohlokoa ka ho bonts'a ka kotloloho ts'ebetso ea kakaretso ea protheine. Haholo-holo, liphatseng tsa lefutso tsa motho, ho feta 85% ea liphetoho tse amanang le mafu li iponahatsa ka har'a libaka tsa protheine ea likhoutu. BMKGENE e fana ka ts'ebeletso e felletseng le e tenyetsehang ea tatellano ea batho ka kakaretso e nang le maano a mabeli a fapaneng a ho hapa a fumanehang ho fihlela lipheo tse fapaneng tsa lipatlisiso.
-
Specific-Locus Amplified Fragment Sequencing (SLAF-Seq)
High-throughput genotyping, haholo-holo ho batho ba bangata, ke mohato oa bohlokoa lithutong tsa liphatsa tsa lefutso 'me o fana ka motheo oa liphatsa tsa lefutso bakeng sa ho sibolloa ha liphatsa tsa lefutso, tlhahlobo ea ho iphetola ha lintho, joalo-joalo.Representation Genome Sequencing (RRGS)hangata e sebelisoa lithutong tsena ho fokotsa litšenyehelo tsa tatellano ea sampuli ka nako e le 'ngoe ha ho ntse ho bolokoa ts'ebetso e nepahetseng ho sibolloeng ke matšoao a lefutso. RRGS e finyella sena ka ho sila DNA e nang le li-enzyme tse thibelang le ho tsepamisa maikutlo ho mefuta e itseng ea boholo ba sekhechana, ka hona e thathamisa karoloana feela ea genome. Har'a mekhoa e fapaneng ea RRGS, Specific-Locus Amplified Fragment Sequencing (SLAF) ke mokhoa o ka khonehang le oa boleng bo holimo. Mokhoa ona, o ntlafalitsoeng o ikemetseng ke BMKGene, o ntlafatsa enzyme ea thibelo e behiloeng bakeng sa morero o mong le o mong. Sena se netefatsa tlhahiso ea palo e kholo ea li-tag tsa SLAF (libaka tse 400-500 bps tsa genome tse hlophisoang) tse ajoang ka mokhoa o ts'oanang ho pholletsa le genome ha ka nepo ho qoba libaka tse iphetang, ka hona, ho netefatsa tšibollo e ntle ka ho fetisisa ea liphatsa tsa lefutso.
-
Illumina lilaebrari tse entsoeng esale pele
Theknoloji ea tatellano ea Illumina, e ipapisitseng le Sequencing by Synthesis (SBS), ke popontšuoa ea lefatše ka bophara ea NGS, e ikarabellang bakeng sa ho hlahisa ho feta 90% ea data ea tatellano ea lefatše. Molao-motheo oa SBS o kenyelletsa ho nahana ka li-terminator tse fetolehang tse ngotsoeng ka fluorescent ha dNTP ka 'ngoe e eketsoa, 'me ka mor'a moo ea thijoa ho lumella ho kenyelletsoa ha setsi se latelang. Ka li-dNTP tse 'ne tse ka fetolehang tse tlamang li-terminator tse teng potolohong e' ngoe le e 'ngoe ea tatellano, tlholisano ea tlhaho e fokotsa leeme la kenyelletso. Theknoloji ena e feto-fetohang e ts'ehetsa lilaebrari tse baloang ha motho a le mong le tse peli, tse sebetsanang le mefuta e mengata ea likopo tsa genomic. Bokhoni ba tatellano e phahameng ea Illumina le ho nepahala bo e beha e le motheo oa lipatlisiso tsa genomics, e matlafatsa bo-ramahlale ba ho manolla boqhetseke ba liphatsa tsa lefutso ka lintlha tse ke keng tsa lekanngoa le bokhabane.
Ts'ebeletso ea rona ea tatellano ea laeborari e entsoeng esale pele e thusa bareki ho hlophisa lilaeborari tse tatellano ho tsoa mehloling e fapaneng (mRNA, genome eohle, amplicon, lilaeborari tsa 10x, hara tse ling). Kamora moo, lilaebrari tsena li ka romelloa litsing tsa rona tsa tatellano bakeng sa taolo ea boleng le tatellano ea li-platform tsa Illumina.
