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BMKMANU S3000_Spatial Transcriptome
Li-Spatial transcriptomics li ka pele ho boqapi ba mahlale, li matlafatsa bafuputsi ho batlisisa mekhoa e rarahaneng ea liphatsa tsa lefutso ka har'a lisele ha ba ntse ba boloka maemo a bona a sebaka. Har'a li-platform tse fapaneng, BMKGene e ntlafalitse BMKManu S3000 Spatial Transcriptome Chip, e ithorisa ka qeto e ntlafalitsoeng ea 3.5µm, e fihlang ho subcellular range, le ho nolofalletsa litlhophiso tsa tharollo ea maemo a mangata. Chip ea S3000, e nang le matheba a ka bang limilione tse 4, e sebelisa li-microwell tse koahetsoeng ka lifaha tse nang le lisebelisoa tsa ho hapa tse nang le barcode. Laeborari ea cDNA, e ntlafalitsoeng ka li-barcode tsa sebaka, e lokisoa ho tsoa ho chip ea S3000 mme ka mor'a moo e hlophisoa sethaleng sa Illumina NovaSeq. Motsoako oa disampole tse nang le barcoded tsa sebaka le li-UMI li netefatsa ho nepahala le ho nepahala ha data e hlahisitsoeng. The BMKManu S3000 chip's e sebetsa ka mokhoa o fapaneng haholo, e fana ka litlhophiso tsa maemo a mangata tse ka hlophisoang hantle le lisele tse fapaneng le lintlha tse batloang. Ho ikamahanya le maemo hona ho beha chip e le khetho e ikhethang bakeng sa lithuto tse fapaneng tsa transcriptomics tsa sebaka, e netefatsang kopano e nepahetseng ea sebaka ka lerata le fokolang. Ts'ebeliso ea mahlale a ho arola lisele ka BMKManu S3000 e thusa ho aroloa ha data ea mongolo ho meeli ea lisele, e leng se hlahisang tlhahlobo e nang le moelelo o tobileng oa tlhaho. Ho feta moo, qeto e ntlafetseng ea S3000 e fella ka palo e phahameng ea liphatsa tsa lefutso le li-UMI tse fumanoang ka sele, e leng ho nolofalletsang tlhahlobo e nepahetseng haholoanyane ea mekhoa ea ho ngola sebaka le ho bokellana ha lisele.
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DNBSEQ lilaeborari tse entsoeng esale pele
DNBSEQ, e ntlafalitsoeng ke MGI, ke theknoloji e ncha ea NGS e khonneng ho fokotsa litšenyehelo tsa tatellano le ho eketsa sephetho. Tokiso ea lilaebrari tsa DNBSEQ e kenyelletsa ho arohana ha DNA, ho lokisoa ha ssDNA, le ho phahamisa selikalikoe ho fumana li-nanoballs tsa DNA (DNB). Ka mor'a moo, tsena li laeloa holim'a sebaka se tiileng 'me ka mor'a moo li hlophisoa ke motsoako oa Probe-Anchor Synthesis (cPAS). Theknoloji ea DNBSEQ e kopanya melemo ea ho ba le tekanyo e tlaase ea liphoso tsa amplification le ho sebelisa mekhoa ea liphoso tse phahameng ka ho fetisisa ka li-nanoballs, e leng se hlahisang tatellano e nang le phallo e phahameng le ho nepahala.
Ts'ebeletso ea rona ea tatellano ea laeborari e entsoeng esale pele e thusa bareki ho lokisa lilaeborari tsa tatellano ea Illumina ho tsoa mehloling e fapaneng (mRNA, genome eohle, amplicon, lilaeborari tsa 10x, har'a tse ling), tse fetoletsoeng ho lilaeborari tsa MGI lilaboratoring tsa rona hore li hlophisoe ho DNBSEQ-T7, e nolofalletsang. litefiso tse phahameng tsa data ka litšenyehelo tse tlase.
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Hi-C e thehiloeng ho Chromatin Interaction
Hi-C ke mokhoa o etselitsoeng ho hapa tlhophiso ea genomic ka ho kopanya litšebelisano tse thehiloeng ho proximity le tatellano ea maemo a holimo. Mokhoa ona o thehiloe ho chromatin crosslinking le formaldehyde, e lateloa ke tšilo ea lijo le ho kopanya hape ka tsela eo likaroloana tse hokahaneng ka kopanelo li tla theha lihlahisoa tsa ligation. Ka tatellano ea lihlahisoa tsena tsa ligation, hoa khoneha ho ithuta mokhatlo oa 3D oa genome. Hi-C e thusa ho ithuta kabo ea likarolo tsa genome tse pakiloeng hanyenyane (A compartments, euchromatin) 'me ho ka etsahala hore e be transcriptionally active, le libaka tse pakiloeng ka thata (B compartments, Heterochromatin). Hi-C e ka boela ea sebelisoa ho supa Topologically Associated Domains (TADs), libaka tsa genome tse nang le meaho e menahaneng 'me ho ka etsahala hore li be le lipaterone tse tšoanang tsa polelo, le ho khetholla li-loops tsa chromatin, libaka tsa DNA tse hokahaneng hammoho ke liprotheine le tse ling. hangata e ruisoa ka likarolo tsa taolo. Ts'ebeletso ea tatellano ea tatellano ea Hi-C ea BMKGene e matlafatsa bafuputsi ho lekola boholo ba sebaka sa genomics, ho bula mekhoa e mecha ea ho utloisisa taolo ea genome le litlamorao tsa eona ho bophelo bo botle le mafu.
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TGuide Smart Magnetic Plant RNA Kit
TGuide Smart Magnetic Plant RNA Kit
Hloekisa kakaretso ea RNA ea boleng bo holimo ho litho tsa semela
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TGuide Smart Mali/Cell/Tissue RNA Kit
TGuide Smart Mali/Cell/Tissue RNA Kit
Thepa e tlatsitsoeng esale pele ea cartridge / plate reagent kit bakeng sa tlhoekiso ea lihlahisoa tse ngata, boleng bo holimo, boleng bo holimo, RNA e se nang inhibitor ho tsoa ho lisele tsa liphoofolo / sele / mali a macha.
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TGuide Smart Magnetic Plant DNA Kit
TGuide Smart Magnetic Plant DNA Kit
Hloekisa DNA ea boleng bo holimo ho tsoa liphatseng tse fapaneng tsa semela
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TGuide Smart Soil / Stool DNA Kit
TGuide Smart Soil / Stool DNA Kit
E hloekisa DNA e se nang inhibitor ea tlhoeko e phahameng le boleng ho tsoa lisampoleng tsa mobu le mantle
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TGuide Smart DNA Purification Kit
E khutlisa DNA ea boleng bo holimo ho tsoa sehlahisoa sa PCR kapa li-gel tsa agarose.
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TGuide Smart Blood Genomic DNA Kit
TGuide Smart Blood Genomic DNA Kit
Thepa e tlatsitsoeng esale pele ea cartridge / plate reagent bakeng sa tlhoekiso ea DNA ea genomic ho tsoa maling le seaparong sa buffy.
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TGuide Smart Magnetic Tissue DNA Kit
Setsi se tlatsitsoeng esale pele sa cartridge / plate reagent bakeng sa ho ntšoa ha DNA ea genomic ho tsoa liseleng tsa liphoofolo
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TGuide Smart Universal DNA Kit
Thepa e tlatsitsoeng esale pele ea cartridge / plate reagent kit bakeng sa tlhoekiso ea genomic DNA maling, sebaka se omisitsoeng sa mali, libaktheria, lisele, mathe, li-swabs tsa molomo, lisele tsa liphoofolo, jj.
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TGuide S16 Nucleic Acid Extractor
TGuide S16 Nucleic Acid Extractor
Sesebelisoa sa Benchtop se bonolo ho se sebelisa, 1-8 Kapa lisampole tse 16 ka nako e le 'ngoe.
Nomoro ea lethathamo / sephutheloana
Katse. che
ID
No. ea preps
OSE-S16-AM
1 sete
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PacBio 2+3 Tharollo ea Bolelele bo Feletseng ba mRNA
Le ha tatellano ea mRNA e thehiloeng ho NGS e le sesebelisoa se feto-fetohang sa ho lekanya liphatsa tsa lefutso, ho ts'epa ha eona lipalo tse khutšoane ho thibela katleho ea eona litlhahlobong tse rarahaneng tsa transcriptomic. Ka lehlakoreng le leng, PacBio sequencing (Iso-Seq) e sebelisa theknoloji e baloang nako e telele, e nolofalletsang tatellano ea lingoloa tsa bolelele bo felletseng ba mRNA. Mokhoa ona o thusa ho hlahlobisisa ka botlalo mekhoa e meng ea ho arola, ho kopanya liphatsa tsa lefutso, le poly-adenylation, leha e se khetho ea mantlha bakeng sa quantification ea liphatsa tsa lefutso. Motsoako oa 2+3 o koala lekhalo pakeng tsa Illumina le PacBio ka ho itšetleha ka PacBio HiFi e bala ho tsebahatsa sete e felletseng ea li-isoform tsa transcript le tatellano ea NGS ho lekanya li-isoform tse ts'oanang.
Platforms: PacBio Sequel II/ PacBio Revio le Illumina NovaSeq;
