Semela / phoofolo ka botlalo
Likarolo tsa lits'ebeletso
● Ho pheha ha laebrari e ka ba tekanyetso kapa pcr-free
● E fumaneha ka li-plats tse 4 tsa tatellano: Illimina novaseq, MGI T7, Nanopore Promethion p48, kapa Pacbio Revoni.
● Tlhahlobo ea bioinformatics e tsepamisitsoeng ho khetholla tse fapaneng: SNP, indel, SV le CNV
LITLHAKISO TSA BOPHELO
●Litlaleho tse ling tse ngata le lingoloa tsa phatlalatso: Boiphihlelo bo bokelletsoeng ka tatellano ea tatellano ea liketsahalo bakeng sa mefuta e fetang 1000 e hlahisitse linyeoe tse fetang 1000 tse hatisitsoeng ka lebaka la tšusumetso e kholo ea ho feta tse tebileng tsa 5000.
●Ho hlahlobisisoa ka botlalo: Ho kenyelletsa le moelelo oa ho letsetsa le ho fana ka tšebetso.
● Tšehetso ea ho rekisa The Post-:Boitlamo ba rona bo atolosa ho feta ho Projeketso ea Projeke ka nako ea ts'ebeletso ea likhoeli tse 3. Ka nako ena, re fana ka thuso ea morero, ho nahanisisa ka thuso le linni ho sebetsana le lintlha tse amanang le liphetho.
●Sengoloa se felletseng: Re sebelisa lithaba tse ngata hore re bolele hore li bua ka mefuta e fapaneng le tse fapaneng li bontša tlhahlobo e lumellanang ea ho ntlafatsa mererong e mengata ea lipatlisiso.
Litlhaloso tsa Tšebeletso
| Ba fapaneng ba lokela ho tsejoa | Leano le thata | Botebo bo khothalelitsoeng |
| Snop le indel | Illimina Novaseq PE150 kapa MGI t7 | 10x |
| SV le CNV (e sa nepahala) | 30x | |
| SV le CNV (E Nepahetseng) | Nanopore Thl P48 | 20x |
| SNP, Indeel, SV le CNV | Pacbio Pacio | 10x |
Litlhoko tsa sampole
| Lithamo kapa li-acid tse nchocho acid | Ilmina / MGI | Nanopore | Pacbio
| ||
| Viscera ea phoofolo | 0.5-1 g | ≥ 3.5 g
| ≥ 3.5 g
| ||
| Mesifa ea phoofolo | ≥ 5 g
| ≥ 5 g
| |||
| Mali a ammal | 1.5 ml | ≥ 0.5 ml
| ≥ 5 ml
| ||
| Mali a likhoho / Litlhapi | ≥ 0.1 ml
| ≥ 0.5 ml
| |||
| Semela lekhasi le lecha | 1-2 g | ≥ 2 g
| ≥ 5 g
| ||
| Lisele tse mongotseng |
| ≥ 1x107
| ≥ 1x108
| ||
| Lisele tse bonolo tsa likokoanyana / motho ka mong | 0.5-1 g | ≥ 1 g
| ≥ 3 g
| ||
| DNA
| TLHOKOMELISO: ≥ 1 NG / μl Chelete: ≥ 30 ng E lekanyelitsoeng kapa e se nang litšila kapa litšila
| TLHOKOMELISO Palo
Od260 / 280
Od260 / 230
E lekanyelitsoeng kapa e se nang litšila kapa litšila
| ≥ 40 NG / μl 4 μg / slow
1.7-2.2
≥1.5 | TLHOKOMELISO Palo
Od260 / 280
Od260 / 230
E lekanyelitsoeng kapa e se nang litšila kapa litšila | ≥ 50 μl / μl 10 μg / Flow Solo / sampole
1.7-2.2
1.8-2.5 |
| Boitokisetso ba Lierekisi tsa PR-Free: Masiba a 40 NG / μl Palo ea 500 ea 500 NG | |||||
Phallo ea Service Flower
Ho tsamaisa Mohlala
DNA Extloction
Kaho ea laebrari
E lekantsoe
Tlhahlobo ea data
Tlhahiso ea data
E kenyelletsa tlhahlobo e latelang:
- Taolo ea boleng bo holimo ea data
- Lipalopalo tsa Alognment ho ea Sebitjhata
- Tekanyetso e fapaneng: SNP, indel, SV le CNV
- Sencintation e sebetsang ea Variants
Lipalopalo tsa Allignment ho litšupiso tsa Genation - Ho Thesela Blaceth
Ho Kena Pina ea Lisahlala Tse Ngata
Boitsebiso ba Indel - Lipalo-palo tsa bolelele ba indel ka setulong sa CD le setereke se sephara
Tsamaiso e fapaneng ea mofuta oa genome - Circos Plot
Tlhahiso e sebetsang ea liphatsa tsa lefutso e nang le phapang e itseng - Tonagy ea Takatso
Chai, Q. Et al. . 433. Doi: 10.1111 / PBI.13965.
Cheng, h. et al. (2023) "CHROMMO HAND SINOREN Doi: 10.1111 / pbi.14018.
Li, A. Et al. . DoI: 10.1038 / S42003-0282333-6.
Zong, T. et al. (2022) Ho hlahlojoa ha maiketsetso le litlolo tsa masapo a Chathy, ho na le leseli la khatello ea nako, biology ea puisano, maq. 1-12, maq. 1-12. 1-12. Doi: 10.1038 / S42003-02283907-7.
