Ha e bue ka litaba tse boletsoeng esale pele tsa Merna-NGs
Likaroloana
● Ho tšoaroa ke motho ea polyna pele ho laebrari
● Itlepetsoeng ea litšupiso tsa ho bua: ho ipapisitse le kopano ea lingoloa, e hlahisang lenane la lingoloa tse ngata tse boletsoeng, cog, kog, kog, kegg)
● Tlhahlobo e akaretsang ea polelo ea Gene le sebopeho sa sengoloa
LITLHAKISO TSA BOPHELO
●Boitsebi bo boholo: Ka rekoto ea pina ea ho lokisa li-sampole tse fetang 600,000 ho Bmkgene, mefuta e fapaneng ea sampole, e sekametseng sampole e fapaneng joalo ka litso tsa sele, li-tuisse, sehlopha sa rona se tlisa leruo morerong le leng le le leng. Re atlehile ho feta merero e fetang 100,000 ea 'marna-liq ka har'a libaka tse fapaneng tsa lipatlisiso.
●Taolo e thata ea boleng: Re kenya taolo ea taolo ea mantlha ka mekhahlelo eohle, ho tloha ka sampole le ho pheha ha laeborari ho e lekanya le e biointoriatics. Ho beha leihlo lena la bokantle ho tiisa ho etsa hore ho be le litholoana tse feto-fetohang litholoana tse fapaneng.
● Ho fana ka litlhaloso tse ngata: Re sebelisa lithaelese tse ngata ho fana ka litlhaloso tse fapaneng tse hlahisoang hampe le ho etsa tlhahlobo e lumellanang, e fanang ka leseli lits'ebetsong tsa cellular le limoleke.
●Ts'ehetso ea Morekisi: Boitlamo ba rona bo atolosa ho feta ho phetheloa ha mosebetsi ka nako ea litšebeletso tse 3. Ka nako ena, re fana ka thuso ea morero, ho nahanisisa ka thuso le linni ho sebetsana le lintlha tse amanang le liphetho.
Litlhoko tsa sampole le ho tsamaisoa
| Laeborari | Leano le thata | Lintlha tse khothalelitsoeng | Taolo ea Boleng |
| Poly arritried | Ilmumina P150 Dnbseq-t7 | 6-10 gb | Q30085% |
Litlhokahalo tsa sampole:
Nucleotives:
| Conc. (NG / μl) | Palo (μg) | Bohloeki | Bothata |
| ≥ 10 | ≥ 0.2 | Od260 / 280 = 1.7-2.5 Od260 / 230 = 0.5-2.5 E lekantsoeng kapa e se nang protheine e bontšitsoeng ka gel. | Bakeng sa limela: rin≥4.0; Bakeng sa liphoofolo: Rin≥4.5; 5.0≥28s / 18s≥ls1.0; LIMITED kapa ha ho na bophahamo ba motheo |
● Lijalo:
Motso, stem kapa petal: 450 mg
Lekhasi kapa peo: 300 mg
Litholoana: 1.2 g
● Phoofolo:
Pelo kapa ka mpeng: 300 mg
Viscera kapa bokong: 240 mg
Muscle: 450 mg
Masapo, moriri kapa letlalo: 1g
● Arthropods:
Likokoanyana: 6g
Crustacea: 300 mg
● Mali a mabeli: 1 tube
● Lisele: 106 Lisele
TLHOKOMELISO EA SMONPLE
Sejana: 2 ML Centrifige Tube (Tin foil ha e khothalletsoe)
A nkatela pele: sehlopha + sa setšoantšo sa A1, A2, A3; B1, B2, B3.
Thomello:
1. Ice-ish: Mehlala e hloka ho paka ka mekotleng ebe o patoa ka leqhoa.
2. Li-tubes tse katiloeng: li-sampole tsa RNA li ka omisoa ho DNA Stabilization tjee (mohlala.
Phallo ea Service Flower
Moralo oa liteko
Ho tsamaisa Mohlala
Rna Eketso
Kaho ea laebrari
E lekantsoe
Tlhahlobo ea data
Litšebeletso tsa ho rekisa ka mor'a ho rekisa
Biolosforformatics
Kopano ea Transcriptome le khetho ea selikalikoe
Sengoloa se sa sebetseng
Mehlala ea lebitso le tlhahlobo ea litšupiso tsa likokoana-hloko
Mefuta e fapaneng e hlahisoang ka tsela e fapaneng
Ts'ebetso e sebetsang ea li-degs tsa Degs
Ts'ebetso ea ts'ebetso ea li-disas
Lekola tsoelo-pele e shebaneng ke Litšebeletso tsa Tlhaho tsa Eukaryotic Ngs Litšebeletso tsa tatellano ea Inten ka har'a lingoliloeng tse khokhothetsoeng likhatisong.
Shen, F. et al. . Doi: 10.1016 / J.YGeno.2020.01.026.
Zhang, C. et al. (2016) Chewana ea ho fana ka mabitso ea metabolism ea tlhaho nakong ea ho ruruha le ho ruruha ha bulb le nts'etsopele ea onion (Allium Cepa L.) 212763. Doi: 10.3389 / FPLS.2016.0142 / BIBTEX.
ZHU, C. et al. (2017) Kopano ea Novo, boqapi le se boleloang bakeng sa sengoliloeng sa Sarkokeigme sa Sinensis ', Pelos One, 12 (2). Doi: 10.1371 / Journal.pone.0171966.
Zou, L. et al. . Doi: 10.1186 / s12870-021-03274-1 / 9.
