mRNA-seq (NGS) e nang le genome
RNA-seq ke sesebelisoa se tloaelehileng ho pholletsa le bophelo le mahlale a lijalo, se koalang lekhalo lipakeng tsa liphatsa tsa lefutso le proteome. Matla a eona a ho sibolla lingoliloeng tse ncha le ho lekanya polelo ea tsona tekong e le 'ngoe. E sebelisoa haholo bakeng sa lithuto tsa papiso ea transcriptomic, e fana ka leseli ho liphatsa tsa lefutso tse amanang le litšobotsi tse fapaneng kapa phenotypes, joalo ka ho bapisa liphetoho le mefuta e hlaha kapa ho hlahisa liphatsa tsa lefutso tlas'a maemo a itseng. BMKCloud mRNA(Reference) APP e kopanya palo ea polelo, tlhahlobo ea polelo e fapaneng (DEG), le sebopeho sa tatelano se sekaseka phaephe ea bioinformatics ea mRNA-seq(NGS) le ho kopanya matla a software e ts'oanang, ho netefatsa boiketlo le botsoalle ba basebelisi. Basebelisi ba ka kenya data ea bona ea RNA-seq marung, moo App e fanang ka tharollo e felletseng ea tlhahlobo ea bioinformatic e le 'ngoe. Ho feta moo, e beha boiphihlelo ba bareki pele, e fana ka ts'ebetso ea motho ka mong e lumellanang le litlhoko tse ikhethileng tsa basebelisi. Basebelisi ba ka beha litlhophiso le ho fana ka thomo ea liphaephe ka bobona, ho lekola tlaleho e sebetsanang, ho sheba data / lits'oants'o le merafo e felletseng ea data, joalo ka: khetho ea liphatsa tsa lefutso, lihlopha tse sebetsang, ho etsa litšoantšo, joalo-joalo.
Sethala:Illumina, MGI
Leano:RNA-Seq
Sebopeho: Lintlha tse pakiloeng, tse hloekileng.
Mofuta oa laeborari:fr-unstranded, fr-firststrand kapa fr-secondstrand
Bolelele ba ho bala:150bp
Mofuta oa faele:*.fastq, *.fq, *.fastq.gz kapa *.fq.gz. Sisteme e tlakopanya lifaele tsa .fastq ho latela mabitso a lifaele,mohlala, *_1.fastq e kopantsoe le *._2.fastq.
Nomoro ea mehlala:Ha ho na lithibelo paloea disampole, empa nako ea tlhahlobo e tla eketseha ha palo eadisampole e hola.
Chelete ea data e khothalelitsoeng:6G ka mohlala