Nanopore ea bolelele bo felletseng ba transcriptome

Nanopore transcriptome sequencing ke mokhoa o matla oa tatellano ea li-cDNA tsa bolelele bo felletseng, ho khetholla ka nepo le ho lekanya li-isoform tsa transcript. Pipeline ea BMKCloud Nanopore Full-Length Transcriptome Pipeline e etselitsoe ho sekaseka lintlha tsa RNA-Seq tse hlahisitsoeng sethaleng sa Nanopore khahlano le genome ea boleng bo holimo, e fanang ka tlhahlobo ea boleng le palo maemong a liphatsa tsa lefutso le sengoloa. Kamora taolo ea boleng, tatelano ea bolelele bo felletseng ba non-chimeric (FLNC) e fumanoa mme tatelano ea tumellano e etsoa 'mapa ho genome ea litšupiso ho tlosa lingoliloeng tse sa hlokahaleng. Ho tsoa sehlopheng sena sa sengoloa, polelo e lekantsoe 'me liphatsa tsa lefutso tse hlahisoang ka mokhoa o fapaneng li khetholloa le ho hlakisoa ka mokhoa o sebetsang. Phaephe e boetse e kenyelletsa tlhahlobo e 'ngoe ea polyadenylation (APA), tlhahlobo e fapaneng ea ho arola, tlhahlobo e bonolo ea tatellano (SSR), ho bolela esale pele ka lncRNA le lipheo tse tsamaellanang, ho bolela esale pele tatellano ea likhoutu (CDS), tlhahlobo ea malapa a lefutso, tlhahlobo ea lintlha tse ngotsoeng, ho bolela mefuta e mecha. le tlhaloso e sebetsang ea lingoliloeng.