mRNA-seq (NGS) - De novo

Tatelano ea mRNA e lumella profiling ea lingoliloeng tsohle tsa mRNA liseleng tlas'a maemo a itseng 'me ke theknoloji e sebelisoang haholo libakeng tse fapaneng tsa lipatlisiso. Pipe ea BMKCloud De novo mRNA-seq e etselitsoe ho sekaseka lilaebrari tse ntlafalitsoeng tsa poly-A ha ho se na genome e fumanehang. Phaephe e qala ka taolo ea boleng, e lateloe kede novokopano ea sengoloa le khetho ea sete ea unigene. Tlhahlobo ea sebopeho sa unigene e bolela esale pele tatellano ea likhoutu (CDS) le pheta-pheto e bonolo ea tatellano (SSR). Kamora moo, tlhahlobo ea polelo e fapaneng e fumana liphatsa tsa lefutso tse hlahisitsoeng ka mokhoa o fapaneng (DEGs) har'a maemo a lekiloeng, a lateloe ke tlhaloso e sebetsang le ntlafatso ea li-DEGs ho ntša lintlha tsa tlhaho.