Hi-C e thehiloeng ho Chromatin Interaction
Likarolo tsa Tšebeletso
● Tatelano ho Illumina NovaSeq le PE150.
● Tšebeletso e hloka lisampole tsa linama, ho e-na le li-nucleic acid tse nkiloeng, hore li hokahane le formaldehyde le ho baballa tšebelisano ea DNA le protheine.
● Teko ea Hi-C e kenyelletsa ho thibela le ho lokisa lipheletso tse khomarelang ka biotin, 'me e lateloe ke ho potoloha ha lipheletso tse lerootho ha ho ntse ho bolokoa litšebelisano. DNA e ntan'o huleloa fatše ka lifaha tsa streptavidin ebe e hloekisoa bakeng sa ho lokisoa ha laebrari e latelang.
Melemo ea Tšebeletso
●Moralo oa Enzyme ea Thibelo e Ntle: ho netefatsa katleho e phahameng ea Hi-C mefuteng e fapaneng e nang le lipara tse sebetsang tsa 93%.
●Litlaleho tse Atolositsoeng tsa Boitsebi le Phatlalatso:BMKGene e na le boiphihlelo bo boholo ka> merero ea tatellano ea Hi-C ea 2000 ho tsoa ho mefuta e fapaneng e 800 le litokelo tse fapaneng tsa litokelo. Linyeoe tse fetang 100 tse hatisitsoeng tse nang le tšusumetso e fetang 900.
●Sehlopha se nang le litsebo tse phahameng tsa Bioinformatics:e nang le litokelo tsa ka tlung le litokelo tsa litokelo tsa software bakeng sa liteko tsa Hi-C le tlhahlobo ea data le software e iketselitsoeng ea data ea pono.
●Tšehetso ea Ka mor'a Thekiso:Boitlamo ba rona bo fetela ka nģ'ane ho ho phethoa ha morero ka nako ea tšebeletso ea likhoeli tse 3 ka mor'a thekiso. Nakong ena, re fana ka tatellano ea projeke, thuso ea ho rarolla mathata, le linako tsa Q&A ho araba lipotso life kapa life tse amanang le sephetho.
●Tlhaloso e Pharalletseng: re sebelisa li-database tse ngata ho hlakisa liphatsa tsa lefutso ka mefuta e fapaneng e tsebahalang le ho etsa tlhahlobo e tsamaellanang ea ntlafatso, ho fana ka leseli mabapi le merero e mengata ea lipatlisiso.
Litlhaloso tsa Tšebeletso
| Laebrari | Leano la Tatelano | Tlhahiso ea data e khothalelitsoeng | Qeto ea Letšoao la Hi-C |
| Laebrari ea Hi-C | Illumina PE150 | Chromatin loop: 150x TAD: 50x | Loop ea Chromatin: 10Kb Tlhaloso: 40Kb |
Litlhoko tsa Tšebeletso
| Mofuta oa mohlala | Chelete e hlokahalang |
| Lisele tsa liphoofolo | ≥2g |
| Madi Kaofela | ≥2mL |
| Li-fungus | ≥1g |
| Semela - lisele tse nyenyane | 1g/aliquot, li-aliquotes tse 2-4 li khothalelitsoe |
| Lisele tsa Cultured | ≥1x107 |
E kenyelletsa Tlhahlobo e Latelang:
● Data e tala QC;
● 'Mapa le laebrari ea Hi-C ea QC: lipara tse nepahetseng tsa ho sebelisana le Interaction Decay Exponents (li-IDE);
● Boitsebiso ba Genome-wide Interaction: tlhahlobo ea cis/trans le 'mapa oa ho sebelisana oa Hi-C;
● Tlhahlobo ea kabo ea phaposi ea A/B;
● Ho khetholla li-TAD le li-loops tsa chromatin;
● Tlhahlobo e fapaneng ho likarolo tsa sebopeho sa chromatin tsa 3D har'a lisampole le tlhaloso e tsamaellanang ea liphatsa tsa lefutso tse amanang.
Cis le trans proportion distribution
Heatmap ea litšebelisano tsa chromosomal lipakeng tsa disampole
Kabo ea likarolo tsa A/B ka bophara ba genome
Phatlalatso e pharalletseng ea li-loops tsa chromatin
Ho bona li-TAD
Lekola tsoelopele ea lipatlisiso e tsamaisoang ke litšebeletso tsa tatellano ea Hi-C ea BMKGene ka pokello e hlophisitsoeng ea lingoliloeng.
Meng, T. et al. (2021) 'Tlhahlobo e bapisoang e kopaneng ea li-multi-omics e supa CA2 e le sepheo sa "chordoma"Neuro-Oncology, 23(10), leqepheng la 1709-1722. doi: 10.1093/NEUONC/NOAB156.
Xu, L. et al. (2021) 'Ho hlophisoa ha 3D le ho hlophisoa bocha ha genome ho fana ka leseli mabapi le pathogenesis ea NAFLD ka tatellano e kopaneng ea Hi-C, Nanopore le RNA',Acta Pharmaceutica Sinica B, 11(10), leqepheng la 3150-3164. doi: 10.1016/J.APSB.2021.03.022.
