TGuide Smart Magnetic Plant RNA Kit
Nomoro ea lethathamo / sephutheloana
| Katse. che | ID | No. ea preps |
| 4993552(khatriji) | DP662-DE | 48 |
Tataiso ea khetho ea reagent bakeng sa TGuide S16
| Asiti ea Nuclei | Mofuta oa Mohlala | Lebitso la Sehlahiswa | Boholo ba ho paka (preps) Cartridge/ poleiti | Nomoro ea katse. Cartridge/ poleiti |
| DNA | Sehlahisoa sa Liphoofolo | TGuide Smart Magnetic Tissue DNA Kit | 48/96 | 4993547/4995038 |
| DNA | Semela & Peō | TGuide Smart Magnetic Plant DNA Kit | 48 | 4993548 |
| DNA | Mobu/Sentle | TGuide Smart Soil /Stool DNA Kit | 48 | 4993549 |
| DNA | Lihlahisoa tsa Gel & PCR | TGuide Smart DNA Purification Kit | 48 | 4993550 |
| DNA | Madi Kaofela | TGuide Smart Blood Genomic DNA Kit | 48/96 | 4993703/4995206 |
| DNA | Cell/Swab/Dry Spots, joalo-joalo | TGuide Smart Universal DNA Kit | 48/96 | 4993704/4995040 |
| RNA | Mali/Seleng/Mahlapa | TGuide Smart Mali/Cell/Tissue RNA Kit | 48/96 | 4993551/4995043 |
| RNA | Semela & Peō | TGuide Smart Magnetic Plant RNA Kit | 48 | 4993552 |
| DNA/RNA | Mekelikeli ea Mahala ea Lisele (Serum, joalo-joalo) | TGuide Smart Viral DNA/RNA Kit | 48/96 | 4993702/4995207 |
Kenyelletso ea sehlahisoa
Hloekisa RNA ea boleng bo holimo, bohloeki bo phahameng, boleng ba boleng bo holimo ho tsoa maling / liseleng / liseleng.
Ha ho li-reagents tse chefo tse kang phenol/chloroform tse nang le tsona
RNA e fumanoeng e na le bohloeki bo phahameng 'me e ka sebelisoa ka ho toba bakeng sa ho lemoha chip, tatellano ea maemo a holimo le liteko tse ling.
Likaroloana
Ho bonolo haholo ho e sebelisa
Ha ho na mosebetsi o eketsehileng oa ho penta.
Bolumo ea elution e ka fetoloa kamoo ho hlokahalang.
Ho kenya le ho sebetsa ho hloka koetliso e fokolang. Ka mananeo a kentsoeng pele, bula cartridge, khetha protocol 'me u tsamaise liteko tsa hau. Mananeo a se a loketse ho sebelisoa, hape a ka etsoa ka mokhoa o ikhethileng.
Li-reagents tse kentsoeng esale pele le lisebelisoa tse ka sebelisoang tse tsamaisanang.
Ho sebetsana le lik'hemik'hale ho felisoa ka tekanyo e kholo ka ho fetisisa ho netefatsa ts'ireletso ea ts'ebetso.
Sephetho sa Demo
Kakaretso ea motso oa koro RNA
Saese ea mohlala: 100 mg
Sample pretreatment: Ho sila ka naetrojene e nang le metsi kapa kalafo ea homogenizer ea mocheso o tlase
Agarose gel concentration :2%(TAE)
Bophahamo ba ho kenya: 2 μl
Lesupa: Letšoao la IV, TIANGEN
Sephetho sa liteko: ho sebelisa TGuide S16 e fihletsoeng ka reagent 4993552 ho ntša ka bo eona RNA eohle ea metso ea koro e na le chai e ntle le bohloeki bo phahameng. Tekong ena, hoo e ka bang 20 μg ea nucleic acid e ntšitsoe metso ea koro ea 100 mg, e nang le OD260 / OD280 ho pota 2.0 ~ 2.1, le OD260 / OD230 = 2.0.
Kakaretso ea RNA ho tsoa lisampoleng tse fapaneng tsa semela
Saese ea mohlala: 100 mg
Mohlala oa pele oa kalafo: mocheso o tlase oa homogenizer
Khatiso ea gel ea Agarose: 1% (TAE)
Bophahamo ba ho kenya: 1 μl
M: Lesupa III, TIANGEN
1-4: peo ea poone 5-8: makhasi a poone 9-12: metso ea k'habeche 13-16: linaoa tsa soya
Mehlala ea pele e 'meli e ile ea ntšoa ka "spin-column extraction kit" mme lisampole tse peli tsa ho qetela li ile tsa ntšoa ke TGuide S16.
Sephetho sa liteko: kotulo ea ho ntšoa ha semela sa RNA ka TGuide S16 e nang le reagent 4993552 e lekana kapa e betere ho feta eo ka "spin-column extraction kit". Bohloeki le botšepehi ha e le hantle lia tšoana. Ka hona, TGuide S16 e ka sebelisoa ho ntša RNA ea semela sebakeng sa tharollo e thehiloeng ho spin-column.
