TGuide Smart Magnetic Plant DNA Kit
Nomoro ea lethathamo / sephutheloana
| Katse. che | ID | No. ea preps |
| 4993548(khatriji) | DP607-DE | 48 |
Tataiso ea khetho ea reagent bakeng sa TGuide S16
| Asiti ea Nuclei | Mofuta oa Mohlala | Lebitso la Sehlahiswa | Boholo ba ho paka (preps) Cartridge/ poleiti | Nomoro ea katse. Cartridge/ poleiti |
| DNA | Sehlahisoa sa Liphoofolo | TGuide Smart Magnetic Tissue DNA Kit | 48/96 | 4993547/4995038 |
| DNA | Semela & Peō | TGuide Smart Magnetic Plant DNA Kit | 48 | 4993548 |
| DNA | Mobu/Sentle | TGuide Smart Soil /Stool DNA Kit | 48 | 4993549 |
| DNA | Lihlahisoa tsa Gel & PCR | TGuide Smart DNA Purification Kit | 48 | 4993550 |
| DNA | Madi Kaofela | TGuide Smart Blood Genomic DNA Kit | 48/96 | 4993703/4995206 |
| DNA | Cell/Swab/Dry Spots, joalo-joalo | TGuide Smart Universal DNA Kit | 48/96 | 4993704/4995040 |
| RNA | Mali/Seleng/Mahlapa | TGuide Smart Mali/Cell/Tissue RNA Kit | 48/96 | 4993551/4995043 |
| RNA | Semela & Peō | TGuide Smart Magnetic Plant RNA Kit | 48 | 4993552 |
| DNA/RNA | Mekelikeli ea Mahala ea Lisele (Serum, joalo-joalo) | TGuide Smart Viral DNA/RNA Kit | 48/96 | 4993702/4995207 |
Kenyelletso ea sehlahisoa
E sebetsa haholo: E loketse mefuta e fapaneng ea li-tissue tsa limela, haholo-holo limela tse nang le polysaccharide-kapa polyphenol-rich.
E bolokehile ebile ha e na chefo: Ha ho li-reagents tse chefo tse kang phenol/chloroform tse teng.
Bohloeki bo phahameng: DNA e fumanoeng e na le bohloeki bo phahameng 'me e ka sebelisoa ka ho toba bakeng sa ho lemoha chip, tatellano ea lintho tse ngata le liteko tse ling.
Likaroloana
Ho bonolo haholo ho e sebelisa
Ha ho na mosebetsi o eketsehileng oa ho penta.
Bolumo ea elution e ka fetoloa kamoo ho hlokahalang.
Ho kenya le ho sebetsa ho hloka koetliso e fokolang. Ka mananeo a kentsoeng pele, bula cartridge, khetha protocol 'me u tsamaise liteko tsa hau. Mananeo a se a loketse ho sebelisoa, hape a ka etsoa ka mokhoa o ikhethileng.
Li-reagents tse kentsoeng esale pele le lisebelisoa tse ka sebelisoang tse tsamaisanang.
Ho sebetsana le lik'hemik'hale ho felisoa ka tekanyo e kholo ka ho fetisisa ho netefatsa ts'ireletso ea ts'ebetso.
Sephetho sa Demo
Ho ntšoa ha DNA ea Genomic ea litapole
Saese ea mohlala: 100 mg
Mohlala oa phekolo ea pele: ho sila ka metsi a naetrojene kapa li-tissue homogenizer
Khatiso ea gel ea Agarose: 2% (TAE)
Bophahamo ba ho kenya: 2 μl
Letšoao: D15000, TIANGEN S16-01, S16-02 le S16-03 li emela liteko tse tšoanang tsa ho ntša semela sa genomic DNA ka ho sebelisa 3 TGuide S16 Nucleic Acid Extractors ka nako e le 'ngoe.
Khamphani: Letšoao le tsebahalang
Sephetho sa liteko: ho sebelisa TGuide S16 e nang le reagent 4993548 ho ntša genomic DNA ea litapole ka bo eona ho fihletse chai e ntle le bohloeki bo phahameng, bo lekanang le boleng ba sehlahisoa sa lisebelisoa tse tsebahalang tsa tlholisano ea spin-column. Tekong ena, hoo e ka bang 11 μg ea nucleic acid e ntšitsoe ho 100 mg ea litapole buds, le OD260 / OD280 ho pota 1.8 ~ 1.9, le OD260 / OD230 = 2.0.
Ho ntšoa ha DNA ea Genomic ea lekhasi la soya
Saese ea mohlala: 100 mg
Mohlala oa phekolo ea pele: ho sila ka metsi a naetrojene kapa li-tissue homogenizer
Khatiso ea gel ea Agarose: 1% (TAE)
Bophahamo ba ho kenya: 2 μl
Lesupa: D15000, TIANGEN
Sephetho sa liteko: ho sebelisa TGuide S16 e nang le reagent 4993548 ho ntša DNA ea genomic ea makhasi a soya ho fihletse chai e ntle le bohloeki bo phahameng. Tekong ena, hoo e ka bang 13μg ea nucleic acid e ntšitsoe makhasi a soya a 100 mg, a nang le OD260 / OD280 ho pota 1.8 ~ 1.9, le OD260 / OD230＞2.0.
Ho ntšoa ha DNA ea Genomic ea metso ea koro
Saese ea mohlala: 100 mg
Sample pre-atreatment: ho sila ka naetrojene e metsi kapa homogenizer e silang lithishu
Khatiso ea gel ea Agarose: 1% (TAE)
Bophahamo ba ho kenya: 2 μl
Lesupa: D15000, TIANGEN
Khamphani: Letšoao le tsebahalang
Sephetho sa teko: ho sebelisa TGuide S16 e nang le reagent 4993548 ho ntša ka boeona DNA ea genomic ea metso ea koro e fihletse chai e ntle le bohloeki bo phahameng, bo lekanang le chai ea ho ntšoa le bohloeki ba bahlolisani ba tsebahalang. Ho feta moo, botšepehi ba DNA ea genomic bo molemo ho feta ba lihlahisoa tse hlōlisanang tsa lik'hamphani tse ling. Tekong ena, hoo e ka bang 15 μg ea nucleic acid e ntšitsoe metso ea koro ea 100 mg, e nang le OD260 / OD280 ho pota 1.8 ~ 1.9, le OD260 / OD230 = 2.0.
