Liphatsa tsa lefutso tsa ho iphetola ha lintho
Melemo ea Tšebeletso
Takagi et al.,Koranta ea semela, 2013
●Tlhahlobo e akaretsang ea bioinformatics:e nolofalletsang khakanyo ea mefuta-futa ea liphatsa tsa lefutso, e bontšang bokhoni ba ho iphetola ha lintho, le ho senola kamano e tšepahalang ea phylogenetic pakeng tsa mefuta e nang le tšusumetso e fokolang ea ho iphetola ha lintho le ho bapisa ho iphetola ha lintho.
●Boikhethelo tlhahlobo e hlophisitsoeng: joalo ka khakanyo ea nako le lebelo la phapano e ipapisitseng le phapang ea boemo ba li-nucleotide le li-amino acid.
●Lirekoto tse pharalletseng tsa Boitsebi le phatlalatso: BMKGene e bokelletse boiphihlelo bo boholo mererong ea palo ea baahi le ea ho iphetola ha lintho ka lilemo tse fetang 15, e akaretsang likete tsa mefuta, joalo-joalo, 'me e kentse letsoho ho feta merero ea boemo bo holimo ea 1000 e phatlalalitsoeng ho Nature Communications, Limela tsa Molecular, Plant Biotechnology Journal, joalo-joalo.
● Sehlopha se nang le litsebo tse phahameng tsa bioinformatics le potoloho e khutšoanyane ea tlhahlobo: ka boiphihlelo bo botle ba tlhahlobo e tsoetseng pele ea genomics, sehlopha sa BMKGene se fana ka litlhahlobo tse felletseng ka nako ea phetoho e potlakileng.
● Tšehetso ea Kamor'a Thekiso:Boitlamo ba rona bo fetela ka nģ'ane ho ho phethoa ha morero ka nako ea tšebeletso ea likhoeli tse 3 ka mor'a thekiso. Nakong ena, re fana ka tatellano ea projeke, thuso ea ho rarolla mathata, le linako tsa Q&A ho araba lipotso life kapa life tse amanang le sephetho.
Litlhaloso tsa Tšebeletso le litlhoko
| Mofuta oa tatellano | Tekanyo ea baahi e khothalelitsoeng | Leano la tatellano | Litlhoko tsa Nucleotide |
| Tatelano ea Genome e Felletseng | ≥ batho ba 30, ba nang le ≥ batho ba 10 ho tsoa sehlopheng se seng le se seng
| 10x | Tsepamiso ea maikutlo: ≥ 1 ng/ µL Kakaretso ea chelete≥ 30ng Ho fokotsoa kapa ho se na seriti kapa tšilafalo |
| Specific-Locus Amplified Fragment (SLAF) | Botebo ba theke: 10x Nomoro ea li-tag: <400 Mb: WGS e khothaletsoa <1Gb: Li-tag tsa 100K 1Gb > 2Gb: Li-tag tsa 300K Boholo ba li-tag tsa 500k | Ho tsepamisa maikutlo ≥ 5 ng/µL Kakaretso ea chelete ≥ 80 ng Nanodrop OD260/280=1.6-2.5 Agarose gel: ha ho na kapa ho senyeha ho fokolang kapa ho silafala
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Phallo ea Mosebetsi oa Ts'ebeletso
Moqapi oa liteko
Mohlala oa thomello
Kaho ea laebrari
Tatelano
Tlhahlobo ea lintlha
Litšebeletso tsa ka mor'a thekiso
Tšebeletso e kenyelletsa tlhahlobo ea sebopeho sa baahi (sefate sa phylogenetic, PCA, chate ea stratification ea baahi), mefuta-futa ea baahi, le khetho ea baahi (ho se lekane ha likhokahano, khetho e khethiloeng ea libaka tse molemo). Ts'ebeletso e ka kenyelletsa tlhahlobo e ikhethileng (mohlala, nako ea ho fapana, phallo ea lefutso).
*Liphetho tsa demo tse bontšitsoeng mona kaofela li tsoa ho liphatsa tsa lefutso tse hatisitsoeng ka BMKGENE
1.Tlhahlobo ea ho iphetola ha lintho e na le kaho ea sefate sa phylogenetic, sebopeho sa baahi le PCA e thehiloeng ho liphatsa tsa lefutso.
Sefate sa Phylogenetic se emela likamano tsa taxonomic le tsa ho iphetola ha lintho har'a mefuta e nang le moholo-holo.
PCA e ikemiselitse ho bona kamano e haufi pakeng tsa batho ba tlase.
Sebopeho sa baahi se bontša boteng ba palo e nyenyane ea liphatsa tsa lefutso ho latela maqhubu a allele.
Chen, le. al.,PNAS, 2020
2.Selective fiela
Khetho e khethollang e bolela mokhoa oo ka oona sebaka se molemo se khethoang le ho eketseha ha libaka tsa libaka tse sa jeleng paate 'me tsa libaka tse sa amaneng li fokotsehile, e leng se bakang phokotso ea libaka.
Ho sibolloa ha Genome ka bophara libakeng tse ikhethileng ho sebetsoa ka ho bala palo ea liphatsa tsa lefutso tsa baahi (π，Fst, Tajima's D) ea li-SNP tsohle ka har'a fensetere e thellang (100 Kb) ka mohato o itseng (10 KB).
Phapang ea Nucleotide (π)
Tajima D
Lenane la ho lokisa(Fst)
Wu, le. al.,Semela sa Molek'hule, 2018
Phallo ea 3.Gene
Wu, le. al.,Semela sa Molek'hule, 2018
Histori ea 4.Demographic
Zhang, le. al.,Nature Ecology & Evolution, 2021
5. Nako ea ho arohana
Zhang, le. al.,Nature Ecology & Evolution, 2021
Lekola tsoelopele e tsamaisitsoeng ke lits'ebeletso tsa tlhabollo ea lefutso tsa BMKGene ka pokello e hlophisitsoeng ea lingoliloeng:
Hassanyar, AK et al. (2023) 'Ho sibolloa ha SNP Molecular Markers le Mokhethoa oa Liphatsa tsa lefutso tse Amahanngoa le Sacbrood Virus Resistance in Apis cerana cerana Larvae by Whole-Genome Resequencing',Koranta ea machaba ea saense ea limolek'hule, 24(7). doi: 10.3390/IJMS24076238.
Chai, J. et al. (2022) 'Ho sibolloa ha salamander ea hlaha, e hloekileng ka liphatsa tsa lefutso tsa China ho theha menyetla e mecha ea paballo',Lipatlisiso tsa Zoological, 2022, Moq. 43, Khatiso ea 3, Maqephe: 469-480, 43 (3), leqepheng la 469-480. doi: 10.24272/J.ISSN.2095-8137.2022.101.
Han, M. et al. (2022) 'Phylogeographical Pattern and Population Evolution History of Indigenous Elymus sibiricus L. on Qinghai-Tibetan Plateau',Frontiers in Plant Science, 13, leq. 882601. doi: 10.3389/FPLS.2022.882601/BIBTEX.
Wang, J. et al. (2022) 'Litlhaloso tsa Genomic mabapi le ho iphetola ha nako e telele ho tsoa kopanong ea genome ea boemo ba chromosome le genomics ea baahi ba nako e telele',Lipatlisiso tsa Temo ea Lirapa, 9. doi: 10.1093/HR/UHAC021.
