Eukaryotic Merna Lequention-NGS
Likaroloana
● Ho tšoaroa ke Poly Monghali pele ho laebrari ea laebrari
● Boikhethelo ba laebrari ea tataiso ea 'Merna
● Tlhahlobo ea bionefortic ea polelo ea Gene le sebopeho sa sengoloa
Melemo
●Boitsebi bo boholoSehlopha sa rona se sebelitse feela sampole e fetang 600,000 ho Bmkgene, e sekama mofuta oa sampole tse kang mefuta ea lisele, lisele le maro a mmele le maro a mmele. Re tlisa leruo le leng le le leng morerong e 'ngoe le e' ngoe, 'me ra phethile ka katleho le merero e fetang 100,000 ea Mrna-Seq ka katleho.
●Taolo e thata ea boleng: Re kenya taolo ea taolo ea mantlha ka mekhahlelo eohle, ho tloha ka sampole le ho pheha ha laeborari ho e lekanya le e biointoriatics. Tlhokomeliso ena e bohlale e tiisa ts'ebetso ea liphetho tse ngata, u u tiisetsang hore morero oa hau o matsohong a tšepahalang.
●Sengoloa se felletseng: Re sebelisa database tse ngata hore re se ke ra bua ka mefuta e fapaneng e hlahisoang ka tsela e fapaneng le ea lihlahlobo tse tsamaellanang. Mokhoa ona o felletseng o fana ka leseli lits'ebetsong tsa cellular le limolek'hule, netefatsa hore u tsebisoa ka botlalo sephetho sa projeke ea hau.
●Ts'ehetso ea Morekisi: Boitlamo ba rona bo atolosa ho feta ho phetheloa ha mosebetsi ka nako ea litšebeletso tse 3. Ka nako ena, re fana ka thuso ea morero, ho nahanisisa ka thuso le linni ho sebetsana le lintlha tse amanang le liphetho.
Litlhoko tsa sampole le ho tsamaisoa
| Laeborari | Leano le thata | Lintlha tse khothalelitsoeng | Taolo ea Boleng |
| Poly arritried | Ilmumina P150 Dnbseq-t7 | 6 -10 GB | Q30085% |
Litlhokahalo tsa sampole:
Nucleotives:
| Conc. (NG / μl) | Palo (μg) | Bohloeki | Bothata | |
| Laeborari e tloaelehileng | ≥ 10 | ≥ 0.2 | Od260 / 280 = 1.7-2.5 Od260 / 230 = 0.5-2.5 E lekantsoeng kapa e se nang protheine e bontšitsoeng ka gel. | Bakeng sa limela: rin≥4.0; Bakeng sa liphoofolo: Rin≥4.5; 5.0≥28s / 18s≥ls1.0; LIMITED kapa ha ho na bophahamo ba motheo |
| Laebrari ea Tataiso | ≥ 10 | ≥ 0.2 | Od260 / 280 = 1.7-2.5 Od260 / 230 = 0.5-2.5 E lekantsoeng kapa e se nang protheine e bontšitsoeng ka gel. | Bakeng sa limela: rin≥4.0; Bakeng sa liphoofolo: Rin≥4.5; 5.0≥28s / 18s≥ls1.0; LIMITED kapa ha ho na bophahamo ba motheo |
● Lijalo:
Motso, stem kapa petal: 450 mg
Lekhasi kapa peo: 300 mg
Litholoana: 1.2 g
●Phoofolo:
Pelo kapa ka mpeng: 300 mg
Viscera kapa bokong: 240 mg
Muscle: 450 mg
Masapo, moriri kapa letlalo: 1g
● Arthropods:
Likokoanyana: 6g
Crustacea: 300 mg
● Mali a mabeli: 1 tube
● Lisele: 106 Lisele
TLHOKOMELISO EA SMONPLE
Sejana: 2 ML Centrifige Tube (Tin foil ha e khothalletsoe)
A nkatela pele: sehlopha + sa setšoantšo sa A1, A2, A3; B1, B2, B3 ......
Thomello:
1. Ice-ish: Mehlala e hloka ho paka ka mekotleng ebe o patoa ka leqhoa.
2. Li-tubes tsa rnas tsa rnas tsa rnas: RNA sampole ea RNA
Phallo ea Service Flower
Moralo oa liteko
Ho tsamaisa Mohlala
Rna Eketso
Kaho ea laebrari
E lekantsoe
Tlhahlobo ea data
Litšebeletso tsa ho rekisa ka mor'a ho rekisa
Biolosforformatics
Eukaryotic Monghali Lekaharantisis
Biolosforformatics
ØTaolo ea boleng bo holimo ea data
ØHo hokahanya ha ngoliseng
ØTlhatlhobo ea Transcript
ØTLHOKOMELISO
ØTlhahlobo e fapaneng ea polelo
ØTlhahiso ea ts'ebetso le ho ruisa
Ho hokahanya ha ngoliseng
Boitsebiso ba data
Mehlala ea lebitso le tlhahlobo ea litšupiso tsa likokoana-hloko
Mefuta e fapaneng e hlahisoang ka tsela e fapaneng
Ts'ebetso e sebetsang ea li-degs tsa Degs
Ts'ebetso ea ts'ebetso ea li-disas
Lekola tsoelo-pele e shebaneng ke Litšebeletso tsa Tlhaho tsa Eukaryotic Ngs Litšebeletso tsa tatellano ea Inten ka har'a lingoliloeng tse khokhothetsoeng likhatisong.
Huang, L. et al. . 119736. Doi: 10.1016 / J.Watres.2023.119736.
Jia, Lj et al. . DoI: 10.1016 / J.Chom.2023.02.002.
Jin, K. et al. . 884443. Doi: 10.3389 / FPLS.2022.8843 / BITOX.
Wen, x. et al. .
Zhang, Yujie et al. . 101798. Doi: 10.1016 / J.Nantod.2023.10171798.
