DNBSEQ lilaeborari tse entsoeng esale pele
Likaroloana
●Sethala:MGI-DNBSEQ-T7
●Mekhoa ea tatellano:PE150
●Phetisetso ea lilaebrari tsa Illumina hoMGI:ho nolofalletsa tatellano ea lipalo tse phahameng tsa data ka theko e tlase.
●Taolo ea boleng ba lilaebrari pele ho tatellano.
●Ho latela data ea QC le ho tsamaisoa:ho fana ka tlaleho ea QC le data e tala ka sebopeho sa fastq ka mor'a ho bala le ho sefa Q30.
Melemo
●Litšebeletso tse fapaneng tsa Sequencing:moreki a ka khetha ho tatellano ka lane kapa palo ea data.
●Tlhahiso ea data e phahameng:1500 Gb / lane
●Tlhahiso ea tlaleho ea tatellano ea QC:ka metrics ea boleng, ho nepahala ha data le ts'ebetso e akaretsang ea morero oa tatellano.
●Ts'ebetso ea tatellano e holileng:ka nako e khuts'oane ea ho tsamaea.
●Taolo ea Boleng e Tiileng: re kenya ts'ebetsong litlhoko tse tiileng tsa QC ho netefatsa ho fana ka liphetho tsa boleng bo holimo kamehla.
Litlhoko tsa Mohlala
| Chelete ea data (X) | Khokahano (qPCR/nM) | Bolumo | |
| Karolo e itseng
| X ≤ 10 Gb | ≥ 1nM | ≥ 25 μl |
| 10 Gb < X ≤ 50 Gb | ≥ 2 nM | ≥ 25 μl | |
| 50 Gb < X ≤ 100 Gb | ≥ 3 nM | ≥ 25 μl | |
| X > 100 Gb | ≥ 4 nM | ||
| Single Lane | Ka Lane | ≥ 1.5 nM / Letamo la libuka | ≥ 25 μl / Letamo la laebrari |
Ntle le ho tsepamisa mohopolo le palo eohle, ho boetse ho hlokahala mohlala o loketseng oa tlhoro.
Tlhokomeliso: Tatelano ea lilaeborari tse fapaneng e hloka hore PhiX e kenelle ho netefatsa hore mohala o matla.
Re khothaletsa ho fana ka lilaeborari tse kopantsoeng esale pele joalo ka mehlala. Haeba o hloka BMKGENE ho etsa pokello ea libuka tsa libuka, ka kopo sheba ho
litlhoko tsa laebrari bakeng sa tatellano e sa fellang ea litselana.
Boholo ba Laebrari ('mapa oa tlhoro)
Tlhōrō e kholo e lokela ho ba ka hare ho 300-450 bp.
Lilaebrari li tlameha ho ba le tlhoro e le 'ngoe e kholo, ha ho na tšilafalo ea adaptara le li-primer dimers.
Ts'ebetso ea ts'ebetso ea litšebeletso
Tlaleho ea Library QC
Tlaleho ea boleng ba laebrari e fanoa pele ho tatellano, ho lekola palo ea laebrari, le ho aroloa.
Ho latela tlaleho ea QC
Letlapa la 1. Lipalopalo tsa tatellano ea lintlha.
| ID ea mohlala | BMKID | E bala e tala | Raw Data (bp) | Ho bala hantle (%) | Q20(%) | Q30(%) | GC(%) |
| C_01 | BMK_01 | 22,870,120 | 6,861,036,000 | 96.48 | 99.14 | 94.85 | 36.67 |
| C_02 | BMK_02 | 14,717,867 | 4,415,360,100 | 96.00 | 98.95 | 93.89 | 37.08 |
Setšoantšo sa 1. Kabo ea boleng hammoho le lipalo ho sampole ka 'ngoe
Setšoantšo sa 2. Kabo ea litaba tsa motheo
Setšoantšo sa 3. Kabo ea litaba tse baloang ka tatellano ea data
