Dna / Rna tatellano - sequenture
Tlelase lintlha
| Sethala | Boholo ba libuka | Sehlooho sa data ea Theoretical (sele) | Ho nepahala ka mokhoa o le mong | Likopo |
| Nanopore | 8kb, 10kb, 20kb, 20kb, Ultralong, CDNA-PR | 70-90GB / sele | 85-92% | SV Call, DeOv, e lekantsoeng ka botlalo e felletseng, Iso-Seq, khanno, ho lemoha ha khan |
LITLHAKISO TSA BOPHELO
● Ho feta boiphihlelo ba lilemo tse 5 ho Pacbio ho sethaleng sa tatellano ea litšoantšo le merero e likete e koetsoeng e nang le mefuta e fapaneng.
● BMKGE ke molekane oa Oxford Nancopore, e nang le Setifikeiti sa RAL sa RA / DNA.
● Ho na le mehlala ea likarolo tse tebileng ka lisebelisoa tse felletseng le ho kenya ka ho lekaneng.
● Ho ipapisitse le sethala sa Nancopore, liphoofolo tse fetang 10 le bafuputsi ba lijalo tse fetang 10 li fetisitsoe makasine ea se nang le phechane ea machabeng.
Litlhoko tsa sampole
| Mofuta oa sampole | Palo | Potlako (Quot ®) | Buka | Bohloeki | Ba bang |
| Tenbic DNA | Ho itšetleha ka tlhokahalo ea data | ≥20ng / μl | ≥15μl | Od260 / 280 = 1.7-2.2; Od260 / 230LS1.5; Hlatsoa tlhoro ho 260 nm, ha ho litšila | Litlhoko tsa mahloriso li tla lekanngoa ke Qubit le Qubit / Nanopore ≤ 2 |
| Kakaretso RNA | ≥1.2μg | ≥100μμlle / μl | ≥15μl | Od260 / 280 = 1.7-2.5; Od260 / 230 = 0.5-2.5; ha ho litšila | Rin boleng ≥7.5 |
Ts'ebeletso ea Ts'ebetso
Mohlala
Kaho ea laebrari
E lekantsoe
Tlhahlobo ea data
TLHOKOMELISO EA PUSELE
Tlhahlobo ea boleng ba data ea data ea DNA ea DNA
Tafole 1. Lipalo-palo ka data e hloekileng.
| Bmkid | Rawseqnum | Rackuble | hloekisaqnseqnum | Pholoso | e hloekile | Pholopa90len | hydmeanlen | sepakapaka | FentManiqual |
| Dna_bmk01 | 1,218,239 | 26.37 | 1,111,736 | 25.90 | 28,014 | 15,764 | 23,090 | 143,181 | 9 |
Tlhahlobo ea boleng ba data ea data ea RNA sampole
Tafole 1. Lipalo-palo ka data e hloekileng.
| Lebitso la faele | Clientid | Readnum | Basenum | N50 | Se bolelang | Madlength | Maluscore |
| Rna_bmk001 | C2 | 8,947,708 | 4,047,230,03303 | 398 | 452 | 129,227 | Q12 |
Setšoantšo sa 1. Bala sebaka sa ho bala
Setšoantšo sa 2. Phetolelo ea boleng ba lintlha tsa data e hloekileng
Setšoantšo sa 3. Bolelele le boleng ba lintlha tsa boleng bo hloekileng
