Illumina lilaebrari tse entsoeng esale pele
Likaroloana
●Platform:Illumina NovaSeq 6000 le NovaSeq X Plus
●Mekhoa ea tatellano:PE150 le PE250
●Taolo ea boleng ba lilaebrari pele ho tatellano
●Ho latela data ea QC le ho tsamaisoa:ho fana ka tlaleho ea QC le data e tala ka sebopeho sa fastq ka mor'a ho bala le ho sefa Q30
Melemo ea Tšebeletso
●Litšebeletso tse fapaneng tsa Sequencing:moreki a ka khetha ho tatellano ka lane, phallo cell, kapa ka bongata ba data e hlokahalang (tekanyo e sa fellang ea lane).
●Boiphihlelo bo pharalletseng sethaleng sa tatellano sa Illumina:ka likete tsa merero e koetsoeng e nang le mefuta e fapaneng.
●Tlhahiso ea tlaleho ea tatellano ea QC:ka metrics ea boleng, ho nepahala ha data le ts'ebetso e akaretsang ea morero oa tatellano.
●Ts'ebetso ea tatellano e holileng:ka nako e khuts'oane ea ho tsamaea.
●Taolo ea Boleng e Tiileng: re kenya ts'ebetsong litlhoko tse tiileng tsa QC ho netefatsa ho fana ka liphetho tsa boleng bo holimo kamehla.
Mehlala ea Platform
| Sethala | Phallo Cell | Mokhoa oa tatellano | Yuniti | Khakanyo ea tlhahiso |
| NovaSeq X | 10B (litsela tse 8) | PE150 | Single Lane Karolo e itseng | 375Gb / Lane |
| 25B (litsela tse 8) | PE150 | Single Lane Karolo e itseng | 1000 Gb / Lane | |
| NovaSeq 6000 | SP Flow cell (litsela tse 2) | PE250 | Phallo Cell Single Lane Karolo e itseng | 325-400 M bala / Lane |
| S4 Flow cell (litsela tse 4) | PE150 | Phallo Cell Single Lane Karolo e itseng | ~800 Gb / Lane |
Litlhoko tsa Mohlala
| Chelete ea data (X) | Khokahano (qPCR/nM) | Bolumo | |
| Sekhechana sa Lane Sequencing
| X ≤ 10 Gb | ≥ 1 nM | ≥ 25 μl |
| 10 Gb < X ≤ 50 Gb | ≥ 2 nM | ≥ 25 μl | |
| 50 Gb < X ≤ 100 Gb | ≥ 3 nM | ≥ 25 μl | |
| X > 100 Gb | ≥ 4 nM | ≥ 25 μl | |
| Lane Sequencing | Ka Lane | ≥ 1.5 nM / Letamo la libuka | ≥ 25 μl / Letamo la laebrari |
Ntle le ho tsepamisa mohopolo le palo eohle, ho boetse ho hlokahala mohlala o loketseng oa tlhoro.
Tlhokomeliso: Tatelano ea lilaeborari tse fapaneng e hloka hore PhiX e kenelle ho netefatsa hore mohala o matla.
Re khothaletsa ho fana ka lilaeborari tse kopantsoeng esale pele joalo ka mehlala. Haeba o hloka BMKGENE ho etsa pokello ea libuka, ka kopo sheba ho
litlhoko tsa laebrari bakeng sa tatellano e sa fellang ea litselana.
Boholo ba Laebrari ('mapa oa tlhoro)
Tlhōrō e kholo e lokela ho ba ka hare ho 300-450 bp.
Lilaebrari li tlameha ho ba le tlhoro e le 'ngoe e kholo, ha ho na tšilafalo ea adaptara le li-primer dimers.
Ka kopo ikopanye le rona haeba disampole tsa hau li sa fihlelle litlhoko tsa ho qala.
Ts'ebetso ea ts'ebetso ea litšebeletso
Taolo ea boleng ba laebrari
Tatelano
Taolo ea boleng ba data
Phano ea morero
Tlaleho ea Library QC
Tlaleho ea boleng ba laebrari e fanoa pele ho tatellano, ho lekola palo ea laebrari, le ho aroloa.
Ho latela tlaleho ea QC
Letlapa la 1. Lipalopalo tsa tatellano ea lintlha.
| ID ea mohlala | BMKID | E bala e tala | Raw Data (bp) | Ho bala hantle (%) | Q20(%) | Q30(%) | GC(%) |
| C_01 | BMK_01 | 22,870,120 | 6,861,036,000 | 96.48 | 99.14 | 94.85 | 36.67 |
| C_02 | BMK_02 | 14,717,867 | 4,415,360,100 | 96.00 | 98.95 | 93.89 | 37.08 |
Setšoantšo sa 1. Kabo ea boleng hammoho le lipalo ho sampole ka 'ngoe
Setšoantšo sa 2. Kabo ea litaba tsa motheo
Setšoantšo sa 3. Kabo ea litaba tse baloang ka tatellano ea data
