Dna / Rna tatellano ea tatellano ea -pacbio
Likaroloana
Mefuta e 'meli ea ho lekanya ho Pacbio Sequencer: E tsoela pele ho bala (CLR) le tumello ea ho bala)
| Mokhoa oa Leoatle | Boholo ba libuka | Lintlha tsa TheoreticalLihlahisoa (sele e le 'ngoe) | Bose-BaseHo nepahala | Likopo |
| KGR | 20kb, 30kb, jj. | 80 GB ho 130 GB | Approx. 85% | De Novo, Sv call, jj. |
| CCS | 15-20 kb | 14 ho isa ho 40 gb / sele (Sequel II) 70 ho isa ho 110 GB / sele (RVIO) Ho latela disampole | Approx. 99% | De Novo, SNP / Indel / SV Call, Iso-Seq, |
Papiso ea ts'ebetso le likarolo tsa Newo le Sequel II
| Lipehelo | Sistimi ea Sequel II Sistimi | Sistimi ea Livio | Nyollelo |
| Letsoalo le phahameng | Li-zmpa tse limilione tse 8 | Li-ZMW tse limilione tse 25 | 3x |
| Mehato e Ikemetseng | 1 | 4 | 4x |
| Nako e khuts'oane ea Mathe | Lihora tse 30 | Lihora tse 24 | 1.25x |
| 30X hifi genomes / Selemo | 88 | 1,300 | 15x ka kakaretso |
LITLHAKISO TSA BOPHELO
● Ho feta seprofearing ea Pacbio equenction e nang le merero e likete e koetsoeng e nang le mefuta e fapaneng.
● E na le tokelo ea ho ba le li-plats tsa morao-rao tsa Pacbio tse sa sebetseng, RVIO ho netefatsa hore ho tla etsahala ka mokhoa o lekaneng.
● Ho bokella nako e potlakileng, ho chesa ho na le lintlha tse nepahetseng le ho feta.
● Bnaketsoa ho makholo a makholo a likopano tse phahameng tsa Pacbio tse thehiloeng ho Pacbio tse thehiloeng ho Pacbio tse thehiloeng ho Pacbio tse thehiloeng ho Pacbio tse thehiloeng ho Pacbio tse thehiloeng ho Pacbio tse thehiloeng ho Pacbio tse thehiloeng ho Pacbio tse thehiloeng ho Pacbio tse thehiloeng ho Pacbio tse thehiloeng ho Pacbio tse thehiloeng ho Pacbio tse thehiloeng ho Pacbio tse thehiloeng ho Pacbio tse thehiloeng ho Pacbio tse thehiloeng ho Pacba
Litlhoko tsa sampole
| Mofuta oa sampole | Palo | Khopolo-taba (Qubit®) | Buka | Bohloeki | Ba bang |
| Tenbic DNA | Ho itšetleha ka tlhokahalo ea data | ≥50 Ng / μl | ≥15μl | Od260 / 280 = 1.7-2.2; Od260 / 230 = 1.8-2.5; Hlakisa tlhoro ho 260 nm,Ha ho litšila | Litlhoko tsa mahloriso tse lokelang ho lekanngoa ka nako ea Qubit le Qubit / Nanopore = 0.8-2.5 |
| Kakaretso RNA | ≥1.2μg | ≥120 ng / μl | ≥15μl | Od260 / 280 = 1.7-2.5; Od260 / 230 = 0.5-2.5;Ha ho litšila | Rin boleng ≥7.5 5028s / 18s≥1 |
Ts'ebeletso ea Ts'ebetso
Mohlala
Kaho ea laebrari
E lekantsoe
Tlhahlobo ea data
TLHOKOMELISO EA PUSELE
Lintlha tsa data tsa morao-rao tsa ntlo
Lintlha tse hlahisitsoeng ho tloha lisele tse 63 tsa CCS (ho tloha ho 26)
| Lintlha-Pacbio-ccs-15 kb | Karolelano | Max | Min | Media |
| Lihlahisoa - liliba (gb) | 421111 | 544.27 | 221.38 | 426.58 |
| Yists - ccs (GB) | 25.93 | 38.59 | 10.86 | 25.43 |
| Polymerase N50 | 145,651 | 175,430 | 118,118 | 144,689 |
| E tlaase n50 | 17,509 | 23,924 | 12,485 | 17,584 |
| CCS N50 | 14,490 | 19,034 | 9,876 | 14,747 |
| Karolelano ea bolelele ba Polymerase | 67,995 | 89,379 | 49,664 | 66,433 |
| Karolelano ea bolelele | 15,866 | 21,036 | 11,657 | 16,012 |
| Karolelano ea bolelele bo bolelele | 14,489 | 19,074 | 8 575 | 14,655 |
Lintlha tse hlahisitsoeng ho tloha lisele tse 16 tsa CRR (ho tloha mefuta ea 76)
| Data-pacbio-clr-30kb | Karolelano | Max | Min | Media |
| Lihlahisoa - liliba (gb) | 142.20 | 291.40 | 50.55 | 142.49 |
| Polymerase N50 | 39,456 | 121,191 | 15,389 | 35,231 |
| E tlaase n50 | 28,490 | 41,012 | 14,430 | 29,063 |
| Karolelano ea bolelele ba Polymerase | 22,063 | 48,886 | 8,747 | 21 555 |
| Karolelano ea bolelele | 17,720 | 27,225 | 8,293 | 17,779 |
2.Data QC - demoLipalo-palo ka kotulo ea data
| Sampole | CCS e bala palo ea linomoro | Likarolo tse felletseng tsa CCS (BP) | CCS e bala N50 (BP) | CCS Bolelele (BP) | CCS e telele ka ho fetisisa e baloang (BP) | Lisosa tsa ka tlase (BP) | Sekhahla sa CCS (%) |
| PB_BMKXXX | 3,444,159 | 54,164,586 586 | 15,728 | 15,726 | 36,110 | 863,326,326,330,465 | 6.27 |
