Bolelele ba bolelele bo felletseng ba 'maquenticing -pacbio
Likaroloana
● CDNA Synthaiss oa Poly-Arna e ile ea lateloa ke litokisetso tsa laebrari
● Lekhotla la CCS CCS, hifi ea bala
● Lengolo la lingoloa tse felletseng
● Tlhaloso ha e na mokhoa oa ho bua ka mokhoa oa ho bua ka mokhoa o fapaneng; Leha ho le joalo, e kanna ea sebelisoa
● Tlhatlhobo ea bioinfortics e thusa ho fana ka litlhahlobo tsa lingoloa lnccrna lnccrna, gesion ea Genel-Aden e hlatsoang hantle, le sebopeho sa gene
LITLHAKISO TSA BOPHELO
●Ho nepahala ho phahameng: HiFi e baloa ka ho nepahala> 99.9% (q30), e ka bapisoang le NGS
● Tlhahlobo e itseng ea ho ithuta: Ha e le hantle ea lingoloa tsohle e thusa ho ts'oana le boits'oaro bo botle le sebopeho
●Boitsebi bo boholo: Ka rekoto ea pina ea ho phethela melaetsa e fetang 1100
●Ts'ehetso ea Morekisi: Boitlamo ba rona bo atolosa ho feta ho phetheloa ha mosebetsi ka nako ea litšebeletso tse 3. Ka nako ena, re fana ka thuso ea morero, ho nahanisisa ka thuso le linni ho sebetsana le lintlha tse amanang le liphetho.
Litlhoko tsa sampole le ho tsamaisoa
| Laeborari | Leano le thata | Lintlha tse khothalelitsoeng | Taolo ea Boleng |
| Polyya e ile ea ntlafatsa laebrari ea CCS | Pacbio Sequele II Pacbio Pacio | 20/40 GB 5/10 M CCS | Q30085% |
Litlhokahalo tsa sampole:
Nucleotives:
● Lijalo:
Motso, stem kapa petal: 450 mg
Lekhasi kapa peo: 300 mg
Litholoana: 1.2 g
● Phoofolo:
Pelo kapa ka mpeng: 300 mg
Viscera kapa bokong: 240 mg
Muscle: 450 mg
Masapo, moriri kapa letlalo: 1g
● Arthropods:
Likokoanyana: 6g
Crustacea: 300 mg
● Mali a mabeli: 1 tube
● Lisele: 106 Lisele
| Conc. (NG / μl) | Palo (μg) | Bohloeki | Bothata |
| ≥ 100 | ≥ 1.0 | Od260 / 280 = 1.7-2.5 Od260 / 230 = 0.5-2.5 E lekantsoeng kapa e se nang protheine e bontšitsoeng ka gel. | Bakeng sa limela: rin≥7.5; Bakeng sa liphoofolo: rin≥8.0; 5.0≥ 28s 1 / 18s≥sv1.0; LIMITED kapa ha ho na bophahamo ba motheo |
TLHOKOMELISO EA SMONPLE
Setshelo: 2 ML Centrifige Tube (TIN FRAIL ha e khothalletsoe)
A nkatela pele: sehlopha + sa setšoantšo sa A1, A2, A3; B1, B2, B3.
Thomello:
1. Ice-ish: Mehlala e hloka ho paka ka mekotleng ebe o patoa ka leqhoa.
2. Li-tubes tse katiloeng: li-sampole tsa RNA li ka omisoa ho DNA Stabilization tjee (mohlala.
Phallo ea Service Flower
Moralo oa liteko
Ho tsamaisa Mohlala
Rna Eketso
Kaho ea laebrari
E lekantsoe
Tlhahlobo ea data
Litšebeletso tsa ho rekisa ka mor'a ho rekisa
E kenyelletsa tlhahlobo e latelang:
● Taolo ea boleng bo holimo ea data
● Tlhahlobo e meng ea Polyadrysis (APA)
● Sekaseka sengoloa sa FUN
● Tlhahlobo e itseng ea ho ithuta
● Benctchmal Universal e le 'ngoe ea litokelo tsa busco)
● Ho hlahlobisisa ha ho hlahlobisisa: ho bolela lintho tse hlakileng tsa ho chechisa maikutlo (li-CDs) le tse sebetsang
● Ho hlahlojoa ha Loncrna: Ho bolela esale pele ho LNcrna le liphofu
● Ho tsebahala ha Mistelitelite (SSR)
TLHOKOMELISO EA BUSCO
Tlhahlobo e meng ea ho qhekella
Tlhahlobo e meng ea Polyadhantion (APA)
Tlhahiso e sebetsang ea mongolo oa phatlalatso
Lekola tsoelo-pele e shebaneng le nanopore ea nanopore ea BMKGene e felletseng ea Intena e felletseng khatisong ena.
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Hua, X. et al. . DoI: 10.1038 / s42003-022222-03000-Z.
Liu, M. Et al. (2023) '' Kopanya 'Pacbio ea Pacbio Iso-liq 363. Doi: 10.3390 / Likokoanyana1440340363 / S1.
Wang, Lijun et al. (2019) Taba ea rarahane e sebelisang pacbio e le 'ngoe ea malebela e kopaneng le Ishala ea khale ea Islimina. DoI: 10.1186 / s12864-019-5832-9.
