Ho hlahlobisisa tlhahlobo e akaretsang
LITLHAKISO TSA BOPHELO
Takagi et al., Koranta ea semela, 2013
● Mamello e Nepahetseng e Kopana le Batho ba 30 + 30 Ho isa ho 200 ho isa ho ba bang ho fokotsa lerata la semelo; polelo e sa lumellaneng le ho bolela esale pele.
● Ho hlahlobisisa ka botlalo: Ho bua ka lentsoe la lipapali la Veno ea Veno, ho kenyelletsa NR, Swispation, E-Kegg, Cog, Kog, Kog, Kog, Kog.
● Nako e potlakileng ea ho fetoha:
● Bothata bo bongata: BMK e kentse letsoho ho likete-kholo ea lehae, ho koahela mefuta e mengata joaloka lijalo tse kang lijalo, lihlahisoa tsa metsing, moru, litholoana tsa litholoana, joalo-joalo
Litlhaloso tsa Tšebeletso
Baahi:
Ho hlalosa batsoali ba nang le maqhubu a phehang.
mohlala F
Pool
Bakeng sa litšobotsi tsa bohlokoa: 30 ho isa ho 50 (bonyane 20) / bulk
Bakeng sa tratis e thata: holimo ho 5% ho batho ba 10% ka ho fetelletsa litaba tse feteletseng ho baahi bohle (bonyane 30 + 30).
Khopolo e Khoesitsoeng
Bonyane 20x / motsoali le 1x / motho ka mong (mohlala, letamo le kopanyang letamo la 30 + 30, botebo bo lekang ka bomong bo tla ba 30x ka bongata)
Bioinformatics ekaseka
● Mora oa mofuta oohle oa lenyalo o tšoana
● Ts'ebetso ea data
● SNP / Indel Call
● Ho hlahlobisisa sebaka sa motho
● Ho fana ka thuto ea batho
Litlhoko tsa sampole le ho tsamaisoa
Litlhokahalo tsa sampole:
Nucleotives:
| Sampole ea Gdna | Sampole ea lisele |
| TLHOKOMELISO: ≥30 NG / μl | Limela: 1-2 g |
| Chelete: ≥2 μg (volmm __5 μl) | Liphoofolo: 0.5-1 g |
| Pearity: od260 / 280 = 1.6-2.5 | Madi ohle: 1.5 ml |
Phallo ea Service Flower
Moralo oa liteko
Ho tsamaisa Mohlala
Rna Eketso
Kaho ea laebrari
E lekantsoe
Tlhahlobo ea data
Litšebeletso tsa ho rekisa ka mor'a ho rekisa
1.Ssoation hases Base ka sebaka sa Euclidean (Ed) ho tseba sebaka sa mokhethoa. Setšoantšong se latelang
X-Axis: Palo ea chromosos; Leqheka le leng le le leng le emela boleng ba ED. Mola o motšo o lumellane le boleng bo lekaneng. Boleng bo phahameng ba EDD bo supa mokhatlo oa bohlokoa o moholo pakeng tsa sebaka le Phenotype. Mohala oa Dash o mofubelu o emela phallo ea mokhatlo oa bohlokoa.
Tlhahlobo ea 2Ssoation ho thehile ha ho na SNP-Index
X-Axis: Palo ea chromosos; DoT e 'ngoe le e' ngoe e emela boleng ba snp. Mohala o motšo o emela boleng bo lekaneng ba sns-index. Ntho e kholo boleng ke, mokhatlo o bohlokoa le ho feta.
Nyeoe ea BMK
Tsebo e kholo ea boleng ba boleng ba boleng ba boleng ba boleng ba
E phatlalalitsoe: Semela biotechnology kopano, 2020
Leano la Leito:
Batsoali (Jin5-50-50-508
Dna matalo a dna (a sekametse hanyane le 50
Liphetho tsa Bohlokoa
Thutong ena, ho ne ho hlahisoa baahi (f2 le f2: 3) e hlahisitsoe ka ho tšela mohala oa likompele jin5-50. Matamo a mabeli a DNA a ne a hahiloe ke batho ba bolelele bo fetang 50 le batho ba 50 ba khuts'oane haholo. QTL e kholo ea QTL e ile ea khetholloa ho Chr07 ke ea litšoaneleho tsa BSA le setso ea QTL. Sebaka sa mokhethoa se ne se felisitsoe ka ho eketsehileng ka mokhoa o motle, palo ea Genel e senotseng Gene ea Bolelele, Csfnl7.1. Ntle le moo, polyrmorphism ho Csfnl7.1 Phetohelo ea ntlafatso e fumanoe e amahanngoa le polelo e lekanang. Tlhahiso ea Phylogeneic e fana ka maikutlo a hore FNL7.1 Loecus e kanna ea simolohile ho tsoa India.
 Qtl-mapping ea BSA ho khetholla sebaka sa mokhethoa se amanang le bolelele ba molala oa likomkomere |  Litlhasi tsa Lod tsa Cucumber Tsoma-Catl li tsejoa ka Chr07 |
Xu, x., et al. "Leqhubu la bohlokoa la boleng Locus FNL7.1 le kenya prosein e kholo ea hoba e amanang le bolelele ba molala likomkomere." Semela biotechnogy kopano 18.7 (2020).
