Kopano ea Baktheria ba sa bakang baktheria
Re fana ka litšebeletso tse felletseng tsa Gecterial Genely Tshebeletso ea kopano ea maiketsetso, e tiisa 0 Gaps. Sena se ka etsahala ka ho kopanya theknoloji ea tatellano ea manonyeletso, joalo ka Nanopore le Pacbio bakeng sa kopano le pherekano ea ho bala ka kopano. Tšebeletso ea rona e fana ka tlaleho e felletseng ea bilinoform ho tsoa phuthehong, e sebetsa le ho itlhahisa e tsamaisitseng boitšoaro bo botle, ho phetha lipheo tse khethehileng tsa lipatlisiso. Tšebeletso ena e thusa nts'etsopele ea Genomes e nepahetseng ea litšupiso tsa lithuto tse fapaneng tsa liphatsa tsa lefutso le tsa Genopic. Ho feta moo, e theha motheo oa lits'ebetso joalo ka ts'ebeletso e tšepahalang, boenjiniere ba liphatsa tsa lefutso, 'me u ntšetsofatsa litekanyetso tsa mahlale le biotechnology.
Likarolo tsa lits'ebeletso
● Na likhetho tse peli tse ka etsang tseo u ka khethang ho latela mohlala o motle oa ho etsa lintho tse felletseng tsa Genemeno.
● Khetho ea Gemect: Ha e bala haufinyane le Itomina Novasetela Novaseqa pe150.
● Tlatsa geneme ea gafe.
● Ho bala ka thata ho Nanopore Promethion 48 kapa Pacbio Pacio bakeng sa kopano ea gene.
● Ho bala ka bokhutšoanyane ho Ilvasena Novasetela bakeng sa Tloaelo ea Gename le ho ntlafatsa (Nanopore) kapa ho hlahisa mofuta oa gena.
LITLHAKISO TSA BOPHELO
●Gero-Gang Geneme e netefalitsoeng: Sena se bakoa ke ho kopana ha Ifumina ho hlobola ka tatellano e telele ea ho bala ha nako e telele ea ho bala (Nanopore kapa pacbio).
●BIAINDFornamianatic:Sena se kenyelletsa kopano ea Genome le Prey of Geneme ea Manonono, Sengoloa sa Khano le Li-poltifation tsa Geneme tse kang Morero oa Circos Plot.
●Boitsebi bo boholo: Ka greomes e fetang 20,000 e tlisa boiphihlelo ka botlalo
●Ts'ehetso ea poso:Boitlamo ba rona bo atolosa ho feta ho Projeketso ea Projeke ka nako ea ts'ebeletso ea likhoeli tse 3. Ka nako ena, re fana ka thuso ea morero, ho nahanisisa ka thuso le linni ho sebetsana le lintlha tse amanang le liphetho.
●Maano a mangata a tatellano ea tatellano ea bohlokoa:Bakeng sa lipheo tse fapaneng tsa lipatlisiso le litlhoko tsa botlalo ba maiketsetso.
Litlhaloso tsa Tšebeletso
| Tshebeletso | Leano le thata |
| Graft genome | Ilmumina pe150 100x |
| 0 gap genome | Nanopore 100x + tirumina pe150 100x Or Pacbio hifi 30x + tirumina pe150 100x (ea boikhethelo) |
Litlhokahalo tsa sampole:
| Khoebo (NG / μl) | Kakaretso (μg) | Buka ea Bongata (μl) | Od260 / 280 | Od260 / 230 | |
| Pacbio | ≥20 | ≥1.2 | ≥20 | 1.7-2.2 | ≥1.0 |
| Nanopore | ≥40 | ≥2 | ≥20 | 1.7-2.2 | 1.0-3.0 |
| Ilmina | ≥1 | ≥0.06 | ≥20 | - | - |
· Bacteria: ≥3.5x1010 Lisele
Phallo ea Service Flower
Ho tsamaisa Mohlala
Kaho ea laebrari
E lekantsoe
Tlhahlobo ea data
Litšebeletso tsa ho rekisa ka mor'a ho rekisa
E kenyelletsa tlhahlobo e latelang:
● Taolo ea boleng bo phahameng ea data
● Bo-raliteng ba Geno
● Litlhahlobo tsa likarolo tsa maiketsetso: Predition ea CD le likarolo tse ngata tsa mahlale
● Moqomo o sebetsang ka libaka tse ngata tse akaretsang (e-ea, Kegg, jj) le liphatlalatso tse tsoetseng pele (karete, VFDB, ETC)
● Tee ea Gilciulization
Re fana ka geneme ka sebopeho sa Fast Fast Fair Fair le faele ea li-aceome (gff).
Sencnotation ea Gene - E-ea
Senotlolo sa khang - lik'habohaedreite tsa cazy
Gename Veufalization - Circos Plot
Lekola tsoelo-pele e shebileng ke Litšebeletso tsa Kopano tsa BMKgene Litšebeletso tsa Kopano tsa GMKGeone ka pokello ea lingoliloeng ka lingoliloeng tse tobileng.
Dai, w. et al. .Mochini oa Moruti oa Machabeng oa Molecular, 24 (19), leq. 14669. Doi: 10,3390 / Ijms2419149699 / S1.
Kang, Q. Et al. .Boemo ba ho hokahana, 16 (6), maq. 798-809. DoI: 10.1111 / 1749-4877.12510.
Wang, TT et al. (2017) Tsela e felletseng ea genome ea Getophyte Bacillus Flexus Klbmp 4941 e senola kholo ea ho holisa setsi sa ho holisa semela sa ho holisa semela le maemo a lefutso bakeng sa mamello ea letsoai ',Koranta ea biotechnology, 260, PP. 38-41. Doi: 10.1016 / J.JbiOTEC.2017.09.001.
Wang, X. et al. .Front in Microbiology, 12, leq. 754931. Doi: 10,33389 / Fmicb.2021.754931 / BITTOX.
