16s / 18s / 18s / Amplicine ea 'nete ea' nete-pacbio
Likarolo tsa lits'ebeletso
● sethala sa bokhelohi: Pacbio rioo
● Mokhoa oa tatellano ea liquence: CCS (hifi e baleha)
● Ho qala sebaka sa sepheo se lateloang ke Tandem ho hokahanya li-amlucon tse ngata pele hifi stll laebrari ea Bell
LITLHAKISO TSA BOPHELO
●Qeto e phahameng ea taxnomic: tHan Short-Amplino,e thusa ho fumana litefiso tse phahameng tsa li-otu tsa OTU sebakeng sa mofuta.
●Ho letsetsa haholo ka ho fetesisa: Pacbio ccs mokhoa oa ho lekanya (hifi e baleha).
●Ho itšehla thajana: Boitsebiso bo potlakileng ba sebopeho sa likoti tsa marang-rang ho sampole ea tikoloho.
●E sebetsa haholo: Lithuto tse fapaneng tsa sechaba.
●Tlhatlhobo e akaretsang ea bilione: Pakete ea morao-rao ea Qime2 (temohisiso ea likhang tse kenang ka lihlahlobo tse fapaneng mabapi le lihlahlobo tsa database, ho fapana le databate
●Boitsebi bo boholo: Ka likete tsa merero ea tlhaselo e senyehileng, BMkgene e hlahisa boiphihlelo ka selemo, sehlopha se ikhethileng, le tšehetso e ntle ea poso.
Litlhaloso tsa Tšebeletso
| Laeborari | Leano le thata | Lintlha tse khothalelitsoeng |
| Amplino | Pacbio Pacio | 10/30/50 KGISS (CCS) |
Litlhoko tsa sampole
| Khoebo (NG / μl) | Kakaretso (μg) | Buka ea Bongata (μl) |
| ≥5 | ≥0.3 | ≥20 |
TLHOKOMELISO EA SMONPLE
Fafatsa mehlala ka nitrojene ea metsi bakeng sa lihora tse 3-4 le lebenkele ka laesense ea naetrojene kapa -80 ho ea sebakeng sa nako e telele. Sampu ea ho romella ka leqhoa le omileng ea hlokahala.
Phallo ea Service Flower
Ho tsamaisa Mohlala
Kaho ea laebrari
E lekantsoe
Tlhahlobo ea data
Litšebeletso tsa ho rekisa ka mor'a ho rekisa
E kenyelletsa tlhahlobo e latelang:
● Taolo ea boleng bo holimo ea data
● Otu Clustering / de-lerata (asv)
● OTUNotation
● Litlhahlobo tsa phapang: Indexes tse ngata, ho kenyeletsa le Shannon, Simpson le Ace.
● Patlo ea Liakete tsa Beta
● Tlhahlobo ea sehlopha ka nako e kenang
● Tlhahlobo ea holimo: Paleng ea tikoloho le sebopeho se kantle le se fapaneng
● 16s e sebetsang e fallisang pan
Sebaka sa thuto ea lipallo
Sefate sa Phylogenetic Sefate
Litlhahlobo tsa Phatlalatso ea Alfation: Ace
index
Tlhatlhobo ea Liakheka tsa Beta: PCOA
Tlhahlobo ea Intergroup: ANOVA
Lekola tsoelo-pele e shebileng ke Litšebeletso tsa lifofane tsa Bmkgene tsa Sequenne le Pacbio ka pokello e khopisitsoeng ea lingoliloeng.
Gao, x. le wang, ho hlahlojoa pele profiles 1), leq. 16.
Li, S. et al. . Doi: 10.1038 / S41522-023339-00439-8.
Mu, L. et al. (2022) Litla-bohloko tsa mafura a mafura a masela a letsoai, khethollo ea ractege e lokiselitsoeng ka li-silage tsa lijo le temo ea li-soane, maq. 1475- 1487. Doi: 10.1002 / JSFA.11482.
Yang, j. et al. . Ho Oxazolone-toloko ea ka mmala o kopane le oxidaven ho batho ba baholo zebrafish ', li-Antioxidants- 2023, Vol. 12, leqepheng la 192, 12 (1), leq. 192. Doi: 10.3390 / Antiox12010192.
