16s / 18s / 18s / Amplicing
Likarolo tsa lits'ebeletso
● sethala sa bokhelohi: Ilumina Novaseq.
● Khelohiloha ea libaka tse khutšoane tsa li-16s tsa 16s, 18s le eona, har'a liphololoana tse ling tsa kalafo.
● Likhetho tse bonolo tsa semotsi.
● Phihlelo ea pele ea morero ka liphofu tse ngata tsa ho qala.
LITLHAKISO TSA BOPHELO
●Ho itšehla thajana:Boitsebiso bo potlakileng ba sebopeho sa likotoana tsa likoti tsa tikoloho.
●Qeto e phahameng: Ka likarolo tse ngata tse tlase tsa sampole ea tikoloho.
●E sebetsa haholo: Lithuto tse fapaneng tsa sechaba.
●Tlhatlhobo e akaretsang ea bilione: Pakete ea morao-rao ea Qime2 (temohisiso ea likhang tse kenang ka lihlahlobo tse fapaneng mabapi le lihlahlobo tsa database, ho fapana le databate
●Boitsebi bo boholo: E nang le merero ea tlhatlhobo e tšoaretsoeng selemo le selemo, BMKGene e hlahisa boiphihlelo ka selemo, sehlopha se ikhethileng, le ts'ehetso e ntle ea thekiso.
Litlhaloso tsa Tšebeletso
| Laeborari | Leano le thata | Lintlha tse khothalelitsoeng |
| Amplino | Ilmina pe250 | Li-tag tsa 50/100 / 300k (tse peli) |
Litlhoko tsa Tšebeletso
| Khoebo (NG / μl) | Kakaretso (NG) | Buka ea Bongata (μl) |
| ≥1 | ≥200 | ≥20 |
● Mobu / Sludge: 1-2g
● Liphoofolo tsa kahare: 0.5-2g
● Litaba tsa ka hare-hare-hare: 0,11.25g
● Semela sa lema (seriti se thehiloe): 0.1-0.5g
● Kenyelle eanke e ntlafalitsoeng): 0.1-0.5g
● Batho ba hae (liphoofolo tse kholo): 0.5-2g
● Batho ba faele (panya): 3-5graress
● Fumary Vavage Fluid Fluid: Pampiri ea Filter
● Sefate sa botšehali: 5-6 Swalls
● Letlalo / sililal swab / Swab / oral soft Tushue / Pharyseaal swab / Ho khutlisa swab: 2-3 swab
● Likokoana-hloko tsa bokaholimo: Thepa ea filthara
● Monna oa metsi / o mong / biofilm: Pampiri ea lifilimi
● Literompeta: 1-2g
● Plaque ea meno: 0.5-1g
Phallo ea Service Flower
Ho tsamaisa Mohlala
Kaho ea laebrari
E lekantsoe
Tlhahlobo ea data
Litšebeletso tsa ho rekisa ka mor'a ho rekisa
E kenyelletsa tlhahlobo e latelang:
- Taolo ea boleng bo holimo ea data
- Otu Clustering / de-lerata (asv)
- Sengoloa sa OTU
- Tlhahlobo ea Phatlalatso ea Alfasion: Li-index ea 'maloa, ho kenyeletsa Shannon, Simpson le Ace.
- Tlhatlhobo ea likhechana tsa Beta
- Tlhahlobo ea sehlopha sa bolulo
- Tlhahlobo ea Khokahano: Patong ea tikoloho le sebopeho sa tikoloho le mefuta e mengata
- 16s e sebetsang ea Typel ea TYEY
Sebaka sa thuto ea lipallo
'Mapa o mongata oa masela
Tlhatlhobo ea Alfalssies: Phello ea Rarefaction
Tlhatlhobo ea likheo tsa Beta: NMDs
Litlhahlobo tsa bolulo: Lefse e sibolla
Lekola tsoelo-pele e shebileng ka lits'ebeletso tsa tatellano ea li-ampine tsa li-amphicne tsa li-amphin tsa li-affinone tse nang le Ilmina ka pokello e khokhothetseng lingoliloeng tse ngata.
Dong, C. Et al. .
Li, y. et al. . Doi: 10.1186 / s040168-023-0141414-y
Yang, j., fu, fu, h. (2022) 'Mano a lerole a koahetsoeng fatše 1-20. Doi: 10.1186 / s0422-022-0399-00399-0 / lipalo / 8.
Yin, s. et al. . 127678. Doi: 10.1016 / J.BIORTTERETE.2022.127678.
