10x genimocnic vium spatial Transcriptome
Morero oa botekgeniki
Likaroloana
● Kutloisiso: 100 μm
● Sebaka sa metsi: 55 μm
● Palo ea libaka: 4992
● Sebaka sa Disture: 6.5 x 6.5 mm
● Sebaka se seng le se seng se tsamaisoa se imeloa ka likomile tse qapiloeng ka likarolo tse 4:
- Poly (DT) mohatla bakeng sa 'Priming le CDNA Synthesis
- Setsebi sa limolecular setsebi sa limolecular (Umi) ho lokisa li-soas tse kholo
- spatial Barcode
- ho tlama ho hokahanya ha karolo e fapaneng ho bala primation
● h & e thetsa likarolo
Melemo
●Tšebeletso e le 'ngoe ea Stop: e kopanya mehato eohle e thehiloeng ho boiphihlelo, ho kenyelletsa likarolo tsa lla, ho pheha khang, ho lokisa ha lipalesa, ho pheha le e bohareng le e biliolengts.
● Sehlopha sa Treeni se nang le tsebo ea bohlokoa: Ka boiphihlelo ba mefuta e fetang 250 le mefuta e 100 ea 100+ ho kenyeletsoa motho, paese, tlhapi le limela le limela le limela.
●Ntlafatso ea nako ea 'nete ho projeke eohle: Ka taolo e felletseng ea tsoelo-pele ea liteko.
●Li-bionorforfortics:Sephutheloana sena se kenyelletsa la 29 oa hlahloba le lipalo tse 100 tsa boleng bo holimo.
●Tlhahlobo ea data e hlophisitsoeng le pono e hlophisitsoeng: E fumaneha bakeng sa likopo tse fapaneng tsa ho etsa lipatlisiso.
●Tlhahlobo ea boikhethelo e kopantsoeng le Cell-Cell Kholo ea Cellna
Litlhaloso
| Litlhoko tsa sampole | Laeborari | Leano le thata | Lintlha tse khothalelitsoeng | Taolo ea Boleng |
| Disam tsa li-dryfo tsa Oct (Bophara bo nepahetseng: Exppar. 6x6x6 mm³) Li-block tse 2 ho sampole | 10x vium vium cdna ha labrary | Ilmumina P150 | 50k pe ho e bala ka leihlo le leng (60gb) | Rin> 7 |
Bakeng sa lintlha tse ling mabapi le tlhaiso ea tataiso le ts'ebeletso ea ts'ebeletso, ka kopo ikutloa u lokolohile ho bua leSetsebi sa BMKgene
Ts'ebeletso ea Ts'ebetso
Mokhasi oa boitokisetso ba mohlala, ho etsoa qeto ea pele ea bulk RNA ho netefatsa hore RNA ea boleng bo holimo e ka fumanoa. Sebakeng sa li-tissie Likarolo li ts'oanelitsoe le maemo a ho bonahala le maemo a amanang le anime ho tsoa tisse. Ebe protocol e ntlafalitsoeng e sebelisoa nakong ea laebrari ea laebrari, e lateloang ke tlhahlobo ea tatellano le ea data.
Ho etsa lintho tse felletseng tsa ts'ebeletso ho kenyelletsa lintlafatso tsa nako ea 'nete le netefatso ea bareki ho boloka senotlolo sa likarabo tse arabelang, ho bonts'a phello ea morero.
E kenyelletsa tlhahlobo e latelang:
Taolo ea boleng ba data:
O Out Of Insupe le Phetolelo ea boleng ba boleng
O Ho lemoha Gene
TLHOKOMELISO E TLANG
Tlhahlobo ea sampole e ka hare:
O Borubo ba Khany
o Spot Cluster, ho kenyelletsa le tlhahlobo e fokotsehileng e fokotsehileng
tlhatlhobo e fapaneng pakeng tsa li-cluster: Ho tsebahala ka liphatsa tsa lefutso
o Ts'ebetso ea ts'ebetso le ho ntlafatsa ea mefuta ea mefuta
Tlhahlobo ea sehlopha sa bolulo
o kopantsoe hape ka li-spots tsa sampole ka bobeli (mohlala. e tšoenyehile ebile e laola) le clasters
Ho tseba lintho tse fapaneng tsa lefutso mabapi le Cluster e 'ngoe le e' ngoe
o Ts'ebetso ea ts'ebetso le ho ntlafatsa ea mefuta ea mefuta
o sesupo se fapaneng sa sehlopha se tšoanang pakeng tsa lihlopha
Tlhahlobo ea sampole e Ikahetseng
Spot Cluss
Matšoao a Lits'ebeletso tsa Lits'ebeletso tsa Lits'ebeletso tsa Lits'ebeletso le Tsamaiso ea Phallo
Tlhahlobo ea sehlopha sa bolulo
Motsoako oa data ho tloha lihlopheng tsena ka bobeli le ho buleha
Mefuta ea matšoao a mefuta e mecha
Hlahloba tsoelo-pele e shebaneng le Ts'ebeletso ea Sepacouri ea Bmkgene
Chen, D. et al. .Linketso tsa National Agademy of Science of America of America, 120 (30), leq. e2303462120. Doi: /10.1073/Pnas.2303462120
Chen, y. et al. .Likhuni ho BioinForciatics, 24 (2), maq. 1-10. Doi: 10.1093 / Bib / Bbad068.
Liu, C. Et al. .Patlisiso ea Nucleic Acids, 50 (17), maq. 9724-9737. Doi: 10.1093 / Nar / gkac773.
Wang, J. Et al. .Moqolotsi oa Machaba oa Machabeng oa Biology, 19 (8), maq. 2515-2530. Doi: 10.7150 / ijbs.83510.
