TGuide S16 Soosaariyaha Ashitada Nukliyeerka
hordhaca alaabta
TGuide S16 Nucleic Acid Extractor ayaa isku mar ka baaraandegi kara ilaa 16 muunado isku mar ah ulaha birlabeedka si ay ugu xidhaan oo ugu wareejiyaan kuul magnetic, saxan 96-ceel ah ama hal muunad reagent cartridge iyo shanlada caarada magnetic. Adigoo isticmaalaya reagent soo saarista aashitada nucleic ee u dhiganta, alaabtani waxay si toos ah DNA/RNA uga nadiifin kartaa noocyo kala duwan oo xayawaan ah, geedo, bakteeriya, fangaska, iwm, waxayna DNA ka soo saari kartaa plasmid, jel agarose iyo PCR.
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Astaamaha
Aad u fudud in la isticmaalo
Ma jirto shaqo tuubo oo dheeraad ah. Mugga elution waxaa loo hagaajin karaa sida loo baahdo.
Rakibaadda iyo hawlgalku waxay u baahan yihiin tababar yar. Barnaamijyada horay loo rakibay, ka soo saar kartoonada, dooro borotokoolka oo socodsii tijaabadaada. Barnaamijyadu waa diyaar in la isticmaalo, sidoo kalena la beddeli karo.
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Qaab cusub oo xidhidh
Usha birlabku waxay qabataa habka xidhitaanka dhamaadka xooggan.
Kuullaha birlabku waxa ay ku xidhaan usha hoose, taas oo hubinaysa in eluent-ku uu wali dabooli karo dhammaan kuulo birlabeedka xataa marka mugga eluentku yar yahay. Naqshadayntu waxay kordhisaa wax-soo-saarka nucleic acid.
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Naqshadaynta ugu fiican ee haanta kuleylka
Waxaa jira tiir la geliyo inta u dhaxaysa lysis buffer iyo tiirka eluent, kaas oo si wax ku ool ah uga hortagaya eluent-ka inuu uumi baxo sababtoo ah kuleylinta tiirarka lysis. Sidaa darteed, mugga soo kabashada ee xalka nucleic acid waa mid sax ah.
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Xakamaynta wasakhda iyo ilaalinta hawlwadeenka
Waxaa la dhigi karaa kursi nadiif ah ama dabool kiimikaad ah.
Qalabku wuxuu u socdaa si toos ah oo xiran.
Nidaamka xakamaynta faddaraynta wuxuu qaataa laba hab: nidaamka xakamaynta faddaraynta gudaha iyo nidaamka jeermiska ultraviolet, si aad uga fogaato is-dhaafsiga u dhexeeya tiirarka iyo muunadaha.
Dib-u-dejiyayaal hore loo raray iyo agabka la tuuri karo
Maaraynta kiimikada waa la tirtiraa ilaa xadka ugu badan si loo xaqiijiyo badbaadada hawlgalka.
Natiijooyinka Demo
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Natiijada tijaabada: addoo isticmaalaya TGuide S16 oo wata qalabka DNA-ga ee TGuide Smart Universal (4993704)
in si toos ah loo soo saaro DNA-da genomic ee shaybaarro badan oo ay ku jiraan unugyo, unugyo, dhiig, barta dhiigga ee qallalan (DBS), Lactobacillus, E.coli, candhuufta, iyo suufka, iwm, si loo gaaro wax soo saar wanaagsan iyo nadiif sare.
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OD260/OD280 agagaarka 1.7 ~ 1.9, iyo
OD260/OD230 ：1.6.
Mugga iftiinka: 100 μl
Feejignaanta Gel Agarose: 1.5%
Soo dejinta mugga: 1 μl
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Isticmaalka TGuide S16 oo wata qalabka reagent TGuide Smart Viral DNA/RNA Kit (4993702)
in si toos ah looga soo saaro DNA/RNA ilo badan oo ay ku jiraan dhiiga oo dhan, suuf iyo balaasmaha ayaa la gaaray dhalooyin wanaagsan iyo nadiif sare. -
OD260/OD280 agagaarka 1.7 ~ 1.9, iyo OD260/OD230 ＞ 1.6.
Shirkad & Shirkad B: Noocyo caan ah.
ND: Cudurka Newcastle, H5: ifilada avian, CP: Canine parvovirus, ASF: Fayraska qandhada doofaarka ee Afrika
Dareenka ugu yar wuxuu gaari karaa 100 nuqul/ml.
Natiijada tijaabada: iyadoo la adeegsanayo TGuide S16 oo leh reagent TGuide Smart Blood Genomic DNA Kit (4993703) si si toos ah loo soo saaro DNA-da genomic ee dhiiga barafaysan waxay gaadhay waxsoosaar wanaagsan iyo nadiif sare, taas oo u dhiganta soo saarista iyo nadiifinta alaabada tartanka caanka ah.
OD260/OD280 agagaarka 1.7 ~ 1.9, iyo OD260/OD230 ＞ 1.7.
Tusaalaha Isha: Saddex qaybood oo dhiig oo dhan oo bini'aadam ah. Saddex nuqul ayaa ah imtixaannada xirmooyinka gaarka ah.
Ku-noqoshada xirmo kasta waxay ka yimaaddeen ilo muunad isku mid ah.
Heerka Tusaalaha: Dhiig barafaysan
Mugga wax-soo-gelinta: 200 μl
Mugga iftiinka: 100 μl
Feejignaanta Gel Agarose: 1.5%
Soo dejinta mugga: 1 μl
M: Marker III, TIANGEN
Shirkad & Shirkad B: Noocyo caan ah
2000 bp qaybta agarose gel DNA sifaynta iyo soo kabashada
Isku-duubnaanta Agarose gel: 1.5% (TBE)
Qaadashada mugga: 8 μl
Calaamadda: D2000, TIANGEN
S16-01, S16-02, iyo S16-03 waxay matalaan muunada isbarbardhiga ee nadiifinta iyo tijaabinta soo kabashada iyadoo la adeegsanayo 3 TGuide S16 Nucleic Acid Extractors oo leh TGuide Smart DNA Sifeynta Kit (4993550) isku mar.
Shirkad: xirmada soo saarista laf-dhabarta-column-ka ee sumad caan ah
Agilent 2100 ayaa loo isticmaalay ogaanshaha hufnaanta RNA.
Tusaalaha isha: CD-1 jiirarka
Xaaladda tusaale: dhiig cusub oo dhan
Cabbirka Tusaalaha: 200 μl
Natiijada tijaabada: wadarta guud ee RNA laga soo saaray dhiiga TGuide S16 oo ku qalabaysan TGuide Smart Blood/Cell/ Tissue RNA Kit (4993551) waxay leedahay wax soo saar wanaagsan iyo nadiif sare, waxayna dabooli kartaa baahiyaha isku xigxiga hoose ee wax soo saarka sare.
Mugga soo dejinta: 1 μl. Beerka ayaa la qasi jiray 5 jeer oo la raray. Feejignaanta Gel Agarose: 1%. Electrophoresis 20 min at 6 V / cm;
M: TIANGEN Marker III
1-4: wadnaha 5-8: beerka 9-12: sambabada 13-16: kelyaha
Labada shay ee ugu horreeya ee unug kasta waxaa soo saaray xirmada soo saarista ku salaysan spin-column, iyo labadii ugu dambeeyay waxaa si toos ah u soo saaray TGuide S16.
Tusaalaha isha: CD-1 jiirarka
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Natiijada tijaabada: DNA-da unugyada xoolaha ee si toos ah u soo saartay TGuide S16 oo ku qalabaysan reagent TGuide Smart Magnetic Tissue DNA Kit (4993547) ayaa leh waxsoosaar sare iyo daahirnimo, taas oo u dhiganta DNA-da ay soo saartay nidaamka soo saarista lafdhabarta ku salaysan. Nadiifintu asal ahaan waa isku mid, oo leh OD260 / OD280 agagaarka 1.7 ~ 1.9 iyo OD260 / OD230> 1.7. Gebogebadii, TGuide S16 waxaa loo isticmaali karaa in laga soo saaro DNA-da genomic ee unugyada xayawaanka beddelka xalka soo saarista ku salaysan lafdhabarta.
Wadarta guud ee soo saarista RNA ee shaybaarada dhirta kala duwan
Cabbirka Tusaalaha: 100 mg
Tusaalaha daawaynta ka hor: homogenizer heerkulka hooseeya
Isku-duubnaanta Agarose gel: 1% (TAE)
Mugga soo dejinta: 1 μl
M: Marker III, TIANGEN
1-4: miraha galleyda 5-8: caleen galleyda 9-12: xididka kaabashka 13-16: digirta soy
Labadii muunad ee hore waxa lagu soo saaray xidhmada soo saarista spin-column-ka labadii muunadood ee u dambeeyayna waxa soo saaray TGuide S16.
Soo saarista DNA-da genomic ee xididdada sarreenka
Cabbirka Tusaalaha: 100 mg
Tusaalaha daawaynta ka hor: ku shiida nitrogen dareere ah ama unug shiidi homogenizer
Isku-duubnaanta Agarose gel: 1% (TAE)
Mugga soo dejinta: 2 μl
Calaamadeeyaha: D15000, TIANGEN
Shirkad: sumad caan ah oo caan ah
Natiijada tijaabada: iyadoo la adeegsanayo TGuide S16 oo leh reagent TGuide Smart Magnetic Plant DNA Kit (4993548) si si toos ah loo soo saaro DNA-da genomic ee xididdada sarreenku waxay gaadhay waxsoosaar wanaagsan iyo nadiifnimo sare, taas oo u dhiganta soo saarista soo saarista iyo nadiifinta tartame caan ah. Waxaa intaa dheer, daacadnimada DNA-da genomic waxay ka fiican tahay alaabta tartanka ee shirkadaha kale. Tijaabadan, qiyaastii 15 μg oo ah aashitada nucleic ayaa laga soo saaray 100 mg oo xididada sarreenka ah, oo leh OD260/OD280 agagaarka 1.8 ~ 1.9, iyo OD260/OD230＞2.0.
