-
DNBSEQ horay loo sameeyay maktabadaha
DNBSEQ, oo ay soo saartay MGI, waa tignoolajiyada cusub ee NGS oo ku guulaysatay inay hoos u dhigto kharashyada isku xigxiga iyo kordhinta wax soo saarka. Diyaarinta maktabadaha DNBSEQ waxay ku lug leedahay kala-goynta DNA-da, diyaarinta ssDNA, iyo wareegga wareegga wareegga si loo helo nanoballs DNA-ga (DNB). Kuwan ayaa markaa lagu raraa dusha adag oo ka dibna la isku xigxigay isku-darka Baadhista-Anchor Synthesis (cPAS). Tiknoolajiyada DNBSEQ waxay isku daraysaa faa'iidooyinka haysashada heerka khaladka cod-weyneysiinta hooseeya iyadoo la adeegsanayo qaababka khaladka cufnaanta sare leh nanoballs, taasoo keentay isku xigxiga leh wax-soo-saarka sare iyo saxnaanta.
Adeegeena isku xigxiga maktabadda ee horay loo sameeyay ayaa u suurtageliya macaamiisha inay diyaariyaan maktabadaha isku xigxiga ee Illumina oo laga keenay ilo kala duwan (mRNA, genome dhan, amplicon, maktabadaha 10x, iyo kuwo kale), kuwaas oo loo beddelo maktabadaha MGI ee shaybaaradayada si loogu kalaxiro DNBSEQ-T7, taasoo awood u siinaysa xaddi xog badan oo kharash yar.
-
Illumina horay loo sameeyay maktabadaha
Tignoolajiyada isku xigxiga ee Illumina, oo ku salaysan Taxane by Synthesis (SBS), waa hal-abuur caalami ah oo la aqbalay NGS, oo mas'uul ka ah soo saarista in ka badan 90% xogta isku xigxiga ee adduunka. Mabda'a SBS waxa ay ku lug leedahay sawirida shucaaca leh ee lagu calaamadiyay joojinta dib loo rogi karo marka dNTP kasta lagu daro, ka dibna la kala qaado si loogu ogolaado ku darida saldhiga xiga. Iyada oo dhammaan afarta dNTP-yada ku xidhan ee dib loo rogi karo ay ku jiraan meerto kasta oo taxane ah, tartanka dabiiciga ahi waxa uu yareeyaa eexda ku darista. Tiknoolajiyadan wax-ku-oolka ah waxay taageertaa maktabadaha hal-akhriska ah iyo kuwa-dhamaadka-dhamaadka ah, iyadoo u adeegaysa noocyo badan oo codsiyo genomic ah. Awoodaha wax soo saarka sare ee Illumina iyo sida saxda ah waxay u dhigayaan inay tahay tiir udub dhexaadka cilmi baarista genomics, taasoo awood siinaysa saynisyahanada inay daaha ka rogaan qalafsanaanta genome-yada oo leh tafatir iyo hufnaan aan isbarbar dhigin.
Adeegeena isku xigxiga maktabadda ee horay loo sameeyay ayaa u suurtageliya macaamiisha inay diyaariyaan maktabado isku xigxiga oo laga keenay ilo kala duwan (mRNA, genenom dhan, amplicon, maktabadaha 10x, iyo kuwo kale). Ka dib, maktabadahaan waxaa loo rari karaa xarumahayada isku xigxiga ee ilaalinta tayada iyo isku xigxiga ee goobaha Illumina.
