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BMKMANU S3000_Spatial Transcriptome
transcriptomics Spatial transcriptomics waxay u taagan tahay safka hore ee hal-abuurnimada sayniska, taasoo awood u siinaysa cilmi-baarayaasha inay dhexgalaan qaababka muujinta hidde-sidaha ee qallafsan ee unugyada dhexdooda iyagoo ilaalinaya xaaladdooda bannaan. Meelo kala duwan oo ay ku jiraan, BMKGene waxay soo saartay BMKManu S3000 Spatial Transcriptome Chip, iyada oo ku faanta xallinta la xoojiyey ee 3.5µm, gaarey tirada unugyada hoose, oo awood u siisay dejinta xallinta heerar badan. Chip-ka S3000, oo leh ku dhawaad 4 milyan oo dhibco ah, ayaa ka shaqeeya microwells oo ay ku dahaaran yihiin kuul ay ku rakiban yihiin baarkin meel bannaan oo lagu soo qabto. Maktabad cDNA ah, oo ay ku hodmeen barcode-yada bannaan, ayaa laga soo diyaariyey chip-ka S3000 oo ka dibna lagu sii raacay madal Illumina NovaSeq. Isku darka shaybaarrada barcodeed ee meel bannaan iyo UMI-yada waxay hubinayaan saxnaanta iyo gaarnimada xogta la sameeyay. Chip-ka BMKManu S3000 waa mid aad u wanagsan, isaga oo bixiya dejinta xallinta heerar kala duwan oo si fiican loogu hagaajin karo unugyo kala duwan iyo heerarka la doonayo ee faahfaahinta. Laqabsigani wuxuu u dhigayaa chip-ka doorasho aad u wanaagsan oo loogu talagalay daraasadaha transcriptomics ee kala duwan, hubinta isku-dhafka boosaska saxda ah oo leh buuq yar. Isticmaalka tignoolajiyada kala qaybinta unugyada ee BMKManu S3000 waxay awood u siinaysaa xadaynta xogta qoraalka ee xuduudaha unugyada, taas oo keenta falanqayn leh macne bayooloji oo toos ah. Intaa waxaa dheer, xallinta la wanaajiyey ee S3000 waxay keenaysaa tiro badan oo hidde-sideyaal ah iyo UMI-yada laga helay unug kasta, taas oo awood u siinaysa falanqayn aad u sax ah oo ku saabsan qaababka qoraallada boosaska iyo ururinta unugyada.
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DNBSEQ horay loo sameeyay maktabadaha
DNBSEQ, oo ay soo saartay MGI, waa tignoolajiyada cusub ee NGS oo ku guulaysatay inay hoos u dhigto kharashyada isku xigxiga iyo kordhinta wax soo saarka. Diyaarinta maktabadaha DNBSEQ waxay ku lug leedahay kala-goynta DNA-da, diyaarinta ssDNA, iyo wareegga wareegga wareegga si loo helo nanoballs DNA-ga (DNB). Kuwan ayaa markaa lagu raraa dusha adag oo ka dibna la isku xigxigay isku-darka Baadhista-Anchor Synthesis (cPAS). Tiknoolajiyada DNBSEQ waxay isku daraysaa faa'iidooyinka haysashada heerka khaladka cod-weyneysiinta hooseeya iyadoo la adeegsanayo qaababka khaladka cufnaanta sare leh nanoballs, taasoo keentay isku xigxiga leh wax-soo-saarka sare iyo saxnaanta.
Adeegeena isku xigxiga maktabadda ee horay loo sameeyay ayaa u suurtageliya macaamiisha inay diyaariyaan maktabadaha isku xigxiga ee Illumina oo laga keenay ilo kala duwan (mRNA, genome dhan, amplicon, maktabadaha 10x, iyo kuwo kale), kuwaas oo loo beddelo maktabadaha MGI ee shaybaaradayada si loogu kalaxiro DNBSEQ-T7, taasoo awood u siinaysa xaddi xog badan oo kharash yar.
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Hi-C ku salaysan isdhexgalka Chromatin
Hi-C waa hab loogu talagalay in lagu qabto qaabeynta genomic iyadoo la isku darayo isdhexgalka ku saleysan dhowaanshaha iyo isku xigxiga-sare. Habka waxa uu ku salaysan yahay iskutallaabta chromatin ee leh formaldehyde, oo ay ku xigto dheefshiidka iyo dib-u-celinta qaab kaliya jajabyada si wadajir ah ugu xiran ayaa samayn doona alaabooyin ligation ah. Iyadoo la raacayo alaabooyinkan ligation, waxaa suurtagal ah in la barto ururka 3D ee genome. Hi-C waxa ay sahlaysa in la barto qaybinta qaybaha genome-ka kuwaas oo si fudud loo soo buuxiyey (Qaybyada A, euchromatin) oo ay u badan tahay in ay si qoraal ah u firfircoon yihiin, iyo gobollada si adag u xidhxidhan (Qaybaha B, Heterochromatin). Hi-C waxa kale oo loo isticmaali karaa in lagu tilmaamo Domains Topologically Associated (TADs), gobollada genome ee leh qaabab isku laabma oo ay u badan tahay in ay leeyihiin qaabab hadal isku mid ah, iyo in la aqoonsado wareegyada chromatin, gobollada DNA ee ay isku dhejiyeen borotiinno iyo kuwan inta badan lagu hodmay xubno nidaamsan. Adeegga isku xigxiga ee BMKGene ee Hi-C waxa uu awood u siiyaa cilmi-baarayaasha in ay sahamiyaan cabbirrada baaxadda genomics-ka, iyaga oo furaya waddooyin cusub oo lagu fahmo nidaamka genome-ga iyo saamaynta ay ku leedahay caafimaadka iyo cudurrada.
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TGuide Smart Magnetic Plant RNA Kit
TGuide Smart Magnetic Plant RNA Kit
Ka nadiifi wadarta guud ee RNA tayada sare leh unugyada dhirta
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TGuide Smart Blood/Cutubka RNA Kit
TGuide Smart Blood/Cutubka RNA Kit
Xirmada reagent-ka hore loo buuxiyey ee loogu talagalay nadiifinta wax-soo-saar sare, nadiif sare, tayo sare leh, wadarta guud ee RNA-horjoogaha-la'aanta ah ee laga helo unugyada xayawaanka/unug/dhiig dhan oo cusub
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Qalabka DNA-da ee Dhirta Birlabka leh ee TGuide
Qalabka DNA-da ee Dhirta Birlabka leh ee TGuide
Ka nadiifi DNA-ga genomic-ga tayada sare leh ee unugyada dhirta kala duwan
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TGuide Smart Ciid / Qalabka DNA ee saxarada
TGuide Smart Ciid / Qalabka DNA ee saxarada
Wuxuu nadiifiyaa DNA-da-horjoogaha-free ee daahirsanaanta iyo tayada sare leh ee ciidda iyo muunadaha saxarada
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Qalabka Sifaynta DNA ee casriga ah ee TGuide
Waxay ka soo kabtaan DNA-da tayada sare leh ee alaabta PCR ama agarose gels
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TGuide Smart Dhiigga Genomic DNA Kit
TGuide Smart Dhiigga Genomic DNA Kit
Xirmada kartoonada / saxanadda hore loo buuxiyey ee loogu talagalay hiddo-wadaha DNA-da sifaynta dhiigga iyo jaakad buffy
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TGuide Smart Magnetic Tissue DNA Kit
Xirmada kartoonada / saxanadda hore loo buuxiyey ee soo saarista DNA-da genomic ee unugyada xoolaha
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TGuide Smart Universal Qalabka DNA
Xirmada reagement ee hore loo buuxiyey oo loogu talagalay in lagu sifeeyo genomic DNA ee dhiigga, barta dhiigga ee qallalan, bakteeriyada, unugyada, candhuufta, suufka afka, unugyada xoolaha, iwm.
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TGuide S16 Soosaariyaha Ashitada Nukliyeerka
TGuide S16 Soosaariyaha Ashitada Nukliyeerka
Qalab Benchtop-ka ah oo si fudud loo isticmaali karo, 1-8 ama 16 muunado isku mar ah
Lambarka buugaagta / baakadaha
Bisad maya
ID
Tirada diyaarinta
OSE-S16-AM
1 set
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PacBio 2+3 Dherer buuxa mRNA Solution
Iyadoo isku xigxiga mRNA-ku salaysan ee NGS ay tahay qalab wax ku ool ah oo lagu qiyaaso muujinta hidda-wadaha, ku tiirsanaanta akhrinta gaagaaban ayaa xaddidaysa waxtarkeeda falanqaynta qoraalka qoraalka ee adag. Dhanka kale, PacBio sequencing (Iso-Seq) waxay shaqaaleysiisaa tignoolajiyada akhriska dheer, taasoo awood u siinaya isku xigxiga qoraallada mRNA-ga oo buuxa. Habkani waxa uu fududeeyaa sahaminta dhamaystiran ee kala qaybsanaanta beddelka ah, isku-dhafka hidda-wadaha, iyo poly-adenylation, in kasta oo aanay ahayn doorashada koowaad ee qiyaasidda hiddaha. Isku dhafka 2+3 wuxuu isku xiraa farqiga u dhexeeya Illumina iyo PacBio isagoo ku tiirsan PacBio HiFi wuxuu akhriyaa si loo aqoonsado jaangooyooyinka dhammaystiran ee qoraalka qoraalka iyo taxanaha NGS si loo qiyaaso isoformyada isku midka ah.
Platforms: PacBio Sequel II/ PacBio Revio iyo Illumina NovaSeq;
