Falanqaynta Ururka Genom-ballaaran
Socodka shaqada
Faa'iidooyinka Adeegga
●Khibrad ballaadhan iyo diiwaannada daabacaadda: iyadoo leh waayo-aragnimo urursan ee GWAS, BMKGene waxay dhamaystirtay boqollaal mashruuc oo noocyo ah oo ku saabsan cilmi-baarista GWAS ee dadweynaha, waxay caawisay cilmi-baarayaasha inay daabacaan in ka badan 100 maqaallo, iyo saameynta isugeynta waxay gaartay 500.
● Falanqaynta bioinformatics oo dhamaystiran: socodka shaqada waxaa ka mid ah falanqaynta ururka SNP-sifada, bixinta qayb ka mid ah hiddo-wadaha murashaxiinta iyo sharraxaaddooda shaqo ee u dhiganta.
●Kooxda bioinformatics xirfad sare leh iyo wareegga falanqaynta gaaban: iyadoo waayo-aragnimo weyn u leh falanqaynta genomics horumarsan, kooxda BMKGene waxay soo bandhigtaa falanqayn dhamaystiran oo leh wakhti degdeg ah.
●Taageerada Iibka Kadib:Ballanqaadkayaga waxa uu dhaafsiisan yahay dhamaystirka mashruuca oo leh 3 bilood oo adeega iibka kadib. Inta lagu jiro wakhtigan, waxaanu bixinaa dabagalka mashruuca, caawinta cilad-raadinta, iyo fadhiyada Q&A si wax looga qabto wixii su'aalo ah ee la xidhiidha natiijooyinka.
Tilmaamaha adeegga iyo shuruudaha
| Nooca isku xigxiga | Qiyaasta dadweynaha ee lagu taliyay | Istaraatiijiyada isku xigxiga | Shuruudaha Nucleotide |
| Taxanaha Genome oo dhan | 200 oo muunado | 10x | Xoog-saarista: ≥ 1 ng/µL Wadarta qaddarka≥ 30ng Xaddidan ama aan hoos u dhicin ama faddarayn |
| Qayb Gaar ah-Locus Amplified Fragment (SLAF) | Tag qoto dheer: 10x Tirada calaamadaha: <400 Mb: WGS ayaa lagula talinayaa <1Gb: 100k tags 1Gb > 2Gb: 300k tags ugu badnaan 500k tags | Xooga saarista ≥ 5 ng/µL Wadarta qaddarka ≥ 80 ng Nandrop OD260/280=1.6-2.5 Agarose gel: maya ama xaddidan hoos u dhac ama wasakh
|
Xulashada agabka
Noocyo kala duwan, nooc-hoosaadyo, dhul-beereedka/bangi-ganacsiga/qoysaska isku-dhafan/khayraadka duur-joogta
Noocyada kala duwan, noocyada kala duwan, dhul-beereedka
Qoyska badh-sib/qoyska-sib-buuxa/khayraadka duurjoogta
Socodka Shaqada Adeegga
Naqshadaynta tijaabada
Tusaalaha keenista
soo saarista RNA
Dhismaha maktabadda
Isku xigxiga
Falanqaynta xogta
Adeegyada iibka kadib
Waxa ku jira falanqaynta soo socota:
- Falanqaynta ururka genome-ballaaran: LM, LMM, EMMAX, qaabka FASTLMM
- Sharaxaada hawleed ee hiddo-wadaha murashaxiinta
Falanqaynta ururka SNP-sifada - Qorshaha Manhattan
Falanqaynta ururka sifo-SNP-QQ
Sahami horumarka ay fududeeyeen BMKGene's de GWAS adeegyada iyada oo loo marayo ururinta daabacaado la soo xulay:
Lv, L. iyo al. (2023) 'Aragti ku saabsan aasaaska hidda-socodka ee dulqaadka ammonia ee garka clam Sinonovacula constricta ee daraasadda ururka genome-wide',Aquaculture, 569, b. 739351. doi: 10.1016/J.AQUACULTURE.2023.739351.
Li, X. iyo al. (2022) 'Falanqaynta Multi-omics ee 398 galmoodka masago foxtail waxay daaha ka qaadayaan gobollada genomic ee la xidhiidha guri-guritaan, sifooyin dheef-shiid kiimikaad, iyo saamaynta anti-bararka',Warshada Molecular, 15(8), bogga 1367–1383. doi: 10.1016/j.molp.2022.07.003.
Li, J. iyo al. (2022) 'Gnome-Wide Association Maabaynta ee Hulless Barely Phenotypes ee deegaanka abaarta',Xuduudaha Sayniska Dhirta, 13, p. 924892. doi: 10.3389/FPLS.2022.924892/BIBTEX.
Zhao, X. iyo al. (2021) 'GmST1, kaas oo soo koobaya sulfotransferase, waxay siisaa iska caabbinta fayraska mosaic soybean G2 iyo G3',Dhirta, Unugyada & Deegaanka, 44(8), bogga 2777-2792. doi: 10.1111/PCE.14066.
