De novo Fungal Genome Assembly
BMKGENE waxay bixisaa xalal kala duwan oo loogu talagalay genome-ka fangas-ka, oo daboolaya baahiyaha cilmi-baarista ee kala duwan iyo dhammaystirka genome-ga la doonayo. Isticmaalka akhrinta gaaban ee Illumina kaligaa waxay ogolaataa abuuritaanka genome qabyo ah. Akhris-gaaban iyo isku xigxiga-akhrinta-dheer iyadoo la adeegsanayo Nanopore ama Pacbio ayaa lagu daraa genome fangas oo aad loo sifeeyay oo leh qulqulo dhaadheer. Waxaa intaa dheer, isku-darka taxanaha Hi-C waxay sii kordhisaa awoodaha, taasoo awood u siinaysa helitaanka genome-heer-koromosoom oo dhammaystiran.
Tilmaamaha Adeegga
Iyada oo leh saddex ikhtiyaar oo suurtagal ah oo laga dooran karo iyada oo ku xidhan heerka la doonayo ee dhammaystirnaanta genome:
● Xulashada Genome-ka Qabyada ah: Isku xigxiga akhris-gaaban ee Illumina NovaSeq PE150.
● Xulashada hidda-socodka Fungal Fine:
Sahanka Genome: Illumina NovaSeq PE150.
Golaha Genome: PacBio Revio (HiFi reads) ama Nanopore PromethION 48.
● Heerarka Chromosome fungal Genome:
Sahanka Genome: Illumina NovaSeq PE150.
Golaha Genome: PacBio Revio (HiFi reads) ama Nanopore PromethION 48.
Ku sii wad ku xidhidhaynta golaha Hi-C
Faa'iidooyinka Adeegga
●Xeelado isku xigxiga oo badan ayaa jira: Ujeedooyinka cilmi-baarista ee kala duwan iyo shuruudaha dhammaystirka genome
●Socodka Shaqada Bioinformatics oo Dhameystiran:Tan waxaa ka mid ah isu-ururinta genome-ga iyo saadaasha xubno badan oo genomic ah, sharraxaadda hidda-wadaha shaqaynaysa, iyo xidhidhaynta joogtada ah.
●Khibrad Balaadhan: Iyadoo in ka badan 12,000 genomes microbial la ururiyey, waxaan keenaysaa in ka badan toban sano oo waayo-aragnimo ah, koox falanqaynta xirfad sare leh, content dhamaystiran, iyo taageero iibka ka dib aad u fiican.
●Taageerada Iibka Kadib:Ballanqaadkayaga waxa uu dhaafsiisan yahay dhamaystirka mashruuca oo leh 3 bilood oo adeega iibka kadib. Inta lagu jiro wakhtigan, waxaanu bixinaa dabagalka mashruuca, caawinta cilad-raadinta, iyo fadhiyada Q&A si wax looga qabto wixii su'aalo ah ee la xidhiidha natiijooyinka.
Tilmaamaha Adeegga
| Adeegga | Istaraatiijiyada Taxanaha | Xakamaynta tayada |
| Qabyada Genome | Illumina PE150 100x | Q30≥85% |
| Genom Fine | Sahanka Genome: Illumina PE150 50 x Golaha: PacBio HiFi 30x ama Nanopore 100x | N50 ≥1Mb（Pacbio Unicellular） N50 ≥2Mb (ONT Unicellular) N50 ≥500kb (Kuwa kale) |
| genome-heer-chromosome | Sahanka Genome: Illumina PE150 50 x Golaha: PacBio HiFi 30x ama Nanopore 100x Golaha Hi-C 100x | Joogista saamiga> 90%
|
Shuruudaha Adeegga
| Xooga saarida (ng/µL) | Tirada guud (µg) | Mugga (µL) | OD260/280 | OD260/230 | |
| PacBio | ≥20 | ≥2 | ≥20 | 1.7-2.2 | ≥1.6 |
| Nanopore | ≥40 | ≥2 | ≥20 | 1.7-2.2 | 1.0-3.0 |
| Illumina | ≥1 | ≥0.06 | ≥20 | - | - |
Fungus Unicellular: ≥3.5x1010 unugyo
Fungus macro: ≥10 g
Socodka Shaqada Adeegga
Tusaalaha keenista
Dhismaha maktabadda
Isku xigxiga
Falanqaynta xogta
Adeegyada iibka kadib
Waxa ku jira Falanqaynta soo socota:
Sahanka Genome:
- Isku xigxiga ilaalinta tayada xogta
- Qiyaasta genome: cabbirka, heterozygosity, walxaha soo noqnoqda
Golaha Fine Genome:
- Taxanaha Xakamaynta Tayada Xogta
- De novoGolaha
- Falanqaynta Qaybaha Genome: Saadaasha CDS iyo xubno badan oo genomic ah
- Sharaxaad shaqaynaysa oo wadata xog ururin guud oo badan (GO, KEGG, iwm.) iyo kayd xogeed horumarsan (CARD, VFDB, iwm.)
Golaha Hi-C:
- Qiimaynta Maktabada Hi-C.
- Contigs xidhidhaynta kooxaynta, dalbashada iyo jihaynta
- Qiimaynta Golaha HI-C: Iyada oo ku saleysan genome tixraaceed iyo khariidadda kulaylka
Sahan genome: qaybinta k-mer
Isugeynta genome: sharraxaadda hidda-wadaha homologous (database NR)
Isugeynta genome: sharraxaadda hidda-wadaha shaqaynaysa (GO)
Sahami horumarka ay fududaysay BMKGene's fungal genome meeting services iyada oo loo marayo ururinta daabacaado la soo xulay.
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