TGuide Smart Magnetic Plant RNA Kit
Lambarka buugaagta / baakadaha
| Bisad maya | ID | Tirada diyaarinta |
| 4993552 (kartridge) | DP662-DE | 48 |
Hagaha xulashada Reagent ee TGuide S16
| Nuclei Acid | Tusaalaha Nooca | Magaca Alaabta | Cabbirka baakadka (diyaarinta) Kartuuji/ saxan | Bisadda maya. Kartuun/saanad |
| DNA | Nudaha Xoolaha | TGuide Smart Magnetic Tissue DNA Kit | 48/96 | 4993547/4995038 |
| DNA | Dhirta & Abuurka | Qalabka DNA-da ee Dhirta Birlabka leh ee TGuide | 48 | 4993548 |
| DNA | Ciid/ Saxaro | TGuide Smart Ciid / Qalabka DNA ee saxarada | 48 | 4993549 |
| DNA | Alaabooyinka jel & PCR | Qalabka Sifaynta DNA ee casriga ah ee TGuide | 48 | 4993550 |
| DNA | Dhiig dhan | TGuide Smart Dhiigga Genomic DNA Kit | 48/96 | 4993703/4995206 |
| DNA | Unug/Swab/Meelaha Qalalan, iwm | TGuide Smart Universal Qalabka DNA | 48/96 | 4993704/4995040 |
| RNA | Dhiiga / Unugyada | TGuide Smart Blood/Cutubka RNA Kit | 48/96 | 4993551/4995043 |
| RNA | Dhirta & Abuurka | TGuide Smart Magnetic Plant RNA Kit | 48 | 4993552 |
| DNA/RNA | Dheecaannada Unugyada Xorta ah (Serum, iwm) | TGuide Smart Viral DNA/RNA Kit | 48/96 | 4993702/4995207 |
hordhaca alaabta
Ka nadiifi wax-soo-saarka sare, nadiif-sare, wadarta guud ee RNA-ga tayada sare leh ee dhiigga/unugga/unugga.
Ma jiraan wax sun ah sida phenol/chloroform oo ku jira
RNA-da la helay waxay leedahay daahirnimo sare waxaana si toos ah loogu isticmaali karaa ogaanshaha chip-ka, isku xigxiga-sare iyo tijaabooyin kale.
Astaamaha
Aad u fudud in la isticmaalo
Ma jirto shaqo tuubo oo dheeraad ah.
Mugga elution waxaa loo hagaajin karaa sida loo baahdo.
Rakibaadda iyo hawlgalku waxay u baahan yihiin tababar yar. Barnaamijyada horay loo rakibay, ka soo saar kartoonada, dooro borotokoolka oo socodsii tijaabadaada. Barnaamijyadu waa diyaar in la isticmaalo, sidoo kalena la beddeli karo.
Dib-u-dejiyayaal hore loo raray iyo agabka la tuuri karo
Maaraynta kiimikada waa la tirtiraa ilaa xadka ugu badan si loo xaqiijiyo badbaadada hawlgalka.
Natiijada Demo
xididka sarreenka wadarta soo saarista RNA
Cabbirka Tusaalaha: 100 mg
Tusaalaha pretreatment: shiidida nitrogen dareere ah ama daawaynta unugyada heerkulka hooseeya
Isku-duubnaanta Agarose gel: 2% (TAE)
Mugga soo dejinta: 2 μl
Calaamadeeyaha: Calaamadeeyaha IV, TIANGEN
Natiijada tijaabada: Isticmaalka TGuide S16 oo lagu gaadhay reagent 4993552 si toos ah loo soo saaro wadarta guud ee RNA ee xididada sarreenku waxay leedahay wax soo saar wanaagsan iyo nadiif sare. Tijaabadan, qiyaastii 20 μg oo ah aashitada nucleic ayaa laga soo saaray 100 mg xididada sarreenka ah, oo leh OD260/OD280 agagaarka 2.0 ~ 2.1, iyo OD260/OD230＞2.0.
Wadarta guud ee soo saarista RNA ee shaybaarada dhirta kala duwan
Cabbirka Tusaalaha: 100 mg
Tusaalaha pretreatment: homogenizer heerkulka hoose
Isku-duubnaanta Agarose gel: 1% (TAE)
Mugga soo dejinta: 1 μl
M: Marker III, TIANGEN
1-4: miraha galleyda 5-8: caleen galleyda 9-12: xididka kaabashka 13-16: digirta soy
Labadii muunad ee hore waxa lagu soo saaray xidhmada soo saarista spin-column-ka labadii muunadood ee u dambeeyayna waxa soo saaray TGuide S16.
Natiijada tijaabada: soo saarista dhirta RNA ee TGuide S16 oo leh reagent 4993552 waxay u dhigantaa ama ka fiican tahay taas xirmada soo saarista lafdhabarta. Daahirnimada iyo daacadnimada asal ahaan waa isku mid. Markaa, TGuide S16 waxaa loo isticmaali karaa in laga soo saaro wadarta guud ee RNA ee dhirta beddelka xalka soo saarista ku salaysan lafdhabarta.
