Tguide Smart Dhiiga / unugyada / unugyada RNA
Lambarka Buugga / Baakadaha
| Bisad. maya | ID | Maya. |
| 4995043 (saxanka) | Dp661-e | 96 |
| 4993551 (baararka) | Dp661-de | 48 |
Hagaha Xulashada Xulashada ee Tguide S16
| Nuclei acid | Nooca muunad | Magaca wax soo saarka | Cabbirka xirxirka (Preps) Karttridge / saxan | Bisad maya. Kartoonada / saxanka |
| Deyr | Cawska xayawaanka | Tguide Smart Magnetic unugyada DNA | 48/96 | 4993547/4999038 |
| Deyr | Warshad & abuur | Tguide Smart Magnetic Dhir DNA | 48 | 4993548 |
| Deyr | Ciidda / saxarada | Carrada Smart ee Smart / Saxarada DNA | 48 | 4993549 |
| Deyr | Gel & PCR-ka PCR | Qalabka nadiifinta ee Tguide Straide DNA | 48 | 4993550 |
| Deyr | Dhiiga oo dhan | Qalabka 'tguide' | 48/96 | 4993703/4995206 |
| Deyr | Unug / SWAB / dhibco dhibco ah, iwm | Tguide Smart Universal DNA | 48/96 | 4993704/49999040 |
| Rasuul | Dhiig / unug / unug | Tguide Smart Dhiiga / unugyada / unugyada RNA | 48/96 | 4993551/4995043 |
| Rasuul | Warshad & abuur | Tguide Smart Magnetic warshad RNNA | 48 | 4993552 |
| DNA / RNA | Cabitaanno bilaash ah oo galka ah (serum, iwm) | Qalabka 'tguide' | 48/96 | 4993702/4995207 |
Hordhaca Waxsoosaarka
Nadiifi dhalidda sare, daahirsanaanta sare, wadarta tayada tayada sare ee dhiigga / unugyada / unugyada.
Ma jiro dib-u-dhacyo sun ah sida phenol / chloroform ay kujiraan
RNA-da la helay waxay leedahay daahir sare waxaana si toos ah loogu isticmaali karaa ogaanshaha chip, is-hagitaan sare iyo tijaabooyin kale.
Astaamaha
Super Way fududahay in la isticmaalo
Ma jiro shaqo dheeri ah oo dheeri ah.
Cabbirka welwelka ayaa loo hagaajin karaa sida loogu baahdo.
Rakibaadda iyo hawlgalku waxay u baahan yihiin tababar yar. Barnaamijyada horay loo rakibay, ka soo horreeya kartoonada, xulo borotokoolka oo soco tijaabadaada. Barnaamijyadu waa kuwo loo adeegsado, oo sidoo kale la isticmaali karo.
Dib-u-habeyn horay loo sii waday iyo kuwa la isbarbardhigayo la tuuro.
Wax ka qabashada kiimikada waxaa lagu baabi'iyaa illaa inta ugu weyn si loo hubiyo amniga hawlgalka.
Natiijooyinka Demo
Alaabtii 2100 waxaa loo adeegsaday ogaanshaha daacadnimada.
Isha Muubada Muunad: Jid-ka-CD-1
Xaaladda muunad: dhiig oo dhan
Cabbirka muunad: 200 μl
Natiijadda Tijaabada: Wadarta RNA-da ee laga soo saaray dhiigga Tguide S16 oo ku qalabeysan reagent 4993551 waxay leedahay dhalid wanaagsan iyo daahirnimo sare, waxayna buuxin kartaa baahiyaha hoos udhaca
Isugeynta soosaarka sare.
Isha Muubada Muunad: Jid-ka-CD-1
Mugga rarida: 1 μl. Beerka waxaa lagu qasi jiray 5 jeer oo xamuul.
Agares Gel wuxuu uruursanayaa: 1%. Electrophoresis-ka 20 min at 6 v / cm;
M: Tiangen sumadeeye III 1-4: wadnaha 5-8:
Beerka 9-12: Sanbacada 13-16: Kelyaha
Labada shay ee ugu horreeya ee unug kasta waxaa soo saaray xirmada soo-saarista ee is-xakamaynta safka, iyo labada ugu dambeysa ee la soo saaray si otomaatig ah by tgaide s16.
Natiijadda tijaabada ah: Wadarta unugyada xayawaanka ee RNA si otomaatig ah ayey u soo saartaa Tguide S16 oo ay ku qalabaysan yihiin Reagent 4993551 waxay leedahay dhalid sare iyo daahir, taas oo ay soo saartay rna-ka dib-u-soo-saarka is-ka-soo-saarida. Daahiyuhu asal ahaan waa isku mid, oo leh OD260 / OD280 qiyaastii 2.0 ~ 2.1 iyo OD260 / OD230.0. Gabagabadii, Tguide S16 waxaa loo isticmaali karaa in lagu soo saaro wadarta guud ee unugyada xoolaha ee ku beddelashada xalka soo saarista ee safka ku saleysan.
