Qalabka DNA-da ee Dhirta Birlabka leh ee TGuide
Lambarka buugaagta / baakadaha
| Bisad maya | ID | Tirada diyaarinta |
| 4993548 (kartridge) | DP607-DE | 48 |
Hagaha xulashada Reagent ee TGuide S16
| Nuclei Acid | Tusaalaha Nooca | Magaca Alaabta | Cabbirka baakadka (diyaarinta) Kartuuji/ saxan | Bisadda maya. Kartuun/saanad |
| DNA | Nudaha Xoolaha | TGuide Smart Magnetic Tissue DNA Kit | 48/96 | 4993547/4995038 |
| DNA | Dhirta & Abuurka | Qalabka DNA-da ee Dhirta Birlabka leh ee TGuide | 48 | 4993548 |
| DNA | Ciid/ Saxaro | TGuide Smart Ciid / Qalabka DNA ee saxarada | 48 | 4993549 |
| DNA | Alaabooyinka jel & PCR | Qalabka Sifaynta DNA ee casriga ah ee TGuide | 48 | 4993550 |
| DNA | Dhiig dhan | TGuide Smart Dhiigga Genomic DNA Kit | 48/96 | 4993703/4995206 |
| DNA | Unug/Swab/Meelaha Qalalan, iwm | TGuide Smart Universal Qalabka DNA | 48/96 | 4993704/4995040 |
| RNA | Dhiiga / Unugyada | TGuide Smart Blood/Cutubka RNA Kit | 48/96 | 4993551/4995043 |
| RNA | Dhirta & Abuurka | TGuide Smart Magnetic Plant RNA Kit | 48 | 4993552 |
| DNA/RNA | Dheecaannada Unugyada Xorta ah (Serum, iwm) | TGuide Smart Viral DNA/RNA Kit | 48/96 | 4993702/4995207 |
hordhaca alaabta
Si ballaaran loo dabaqi karo: Waxay ku habboon tahay noocyada kala duwan ee unugyada dhirta, gaar ahaan polysaccharide-ama dhirta hodanka ah ee polyphenol.
Ammaan ah oo aan sun ahayn: Ma jiraan reagenyaal organic sun ah sida phenol/chloroform ka kooban yahay.
Nadiifin sare: DNA-da la helay ayaa leh nadiif sare waxaana si toos ah loogu isticmaali karaa ogaanshaha jajabka, isku xigxiga sare iyo tijaabooyin kale.
Astaamaha
Aad u fudud in la isticmaalo
Ma jirto shaqo tuubo oo dheeraad ah.
Mugga elution waxaa loo hagaajin karaa sida loo baahdo.
Rakibaadda iyo hawlgalku waxay u baahan yihiin tababar yar. Barnaamijyada horay loo rakibay, ka soo saar kartoonada, dooro borotokoolka oo socodsii tijaabadaada. Barnaamijyadu waa diyaar in la isticmaalo, sidoo kalena la beddeli karo.
Dib-u-dejiyayaal hore loo raray iyo agabka la tuuri karo
Maaraynta kiimikada waa la tirtiraa ilaa xadka ugu badan si loo xaqiijiyo badbaadada hawlgalka.
Natiijada Demo
Genomic DNA soo saarista baradhada
Cabbirka Tusaalaha: 100 mg
Tusaalaha daawaynta ka hor: ku shiida nitrogen dareere ah ama homogenizer unug
Isku-duubnaanta Agarose gel: 2% (TAE)
Mugga soo dejinta: 2 μl
Calaamadeeyaha: D15000, TIANGEN S16-01, S16-02 iyo S16-03 waxay u taagan yihiin tijaabooyin isbarbar socda oo laga soo saarayo DNA-ga dhirta iyadoo la isticmaalayo 3 TGuide S16 Nucleic Acid Extractors isku mar.
Shirkad: sumad caan ah oo caan ah
Natiijada tijaabada: iyadoo la adeegsanayo TGuide S16 oo leh reagent 4993548 si toos ah loo soo saaro DNA-da genomic ee baradhada waxay gaadhay waxsoosaar wanaagsan iyo nadiif sare, taas oo u dhiganta tayada wax soo saarka ee xirmooyinka soo saarista lafdhabarta ee caanka ah. Tijaabadan, qiyaastii 11 μg oo ah aashitada nucleic ayaa laga soo saaray 100 mg burooyinkeeda baradhada, oo leh OD260/OD280 agagaarka 1.8 ~ 1.9, iyo OD260/OD230＞2.0.
Soo saarista DNA-da genomic ee caleen soybean
Cabbirka Tusaalaha: 100 mg
Tusaalaha daawaynta ka hor: ku shiida nitrogen dareere ah ama homogenizer unug
Isku-duubnaanta Agarose gel: 1% (TAE)
Mugga soo dejinta: 2 μl
Calaamadeeyaha: D15000, TIANGEN
Natiijada tijaabada: iyadoo la adeegsanayo TGuide S16 oo leh reagent 4993548 si toos ah loo soo saaro DNA-da genomic ee caleemaha soybean waxay gaadhay wax soo saar wanaagsan iyo nadiif sare. Tijaabadan, qiyaastii 13μg oo ah acid nucleic ayaa laga soo saaray 100 mg caleemaha soybean, oo leh OD260/OD280 agagaarka 1.8 ~ 1.9, iyo OD260/OD230＞2.0.
Soo saarista DNA-da genomic ee xididdada sarreenka
Cabbirka Tusaalaha: 100 mg
Tusaalaha daawaynta ka hor: ku shiida nitrogen dareere ah ama unug shiidi homogenizer
Isku-duubnaanta Agarose gel: 1% (TAE)
Mugga soo dejinta: 2 μl
Calaamadeeyaha: D15000, TIANGEN
Shirkad: sumad caan ah oo caan ah
Natiijooyinka tijaabada: iyadoo la adeegsanayo TGuide S16 oo leh reagent 4993548 si toos ah loo soo saaro DNA-da genomic ee xididdada sarreenku waxay gaadhay waxsoosaar wanaagsan iyo daahirnimo sare, taas oo u dhiganta soosaarka soosaarka iyo nadiifinta tartamayaasha caanka ah. Waxaa intaa dheer, daacadnimada DNA-da genomic waxay ka fiican tahay alaabta tartanka ee shirkadaha kale. Tijaabadan, qiyaastii 15 μg oo ah aashitada nucleic ayaa laga soo saaray 100 mg oo xididada sarreenka ah, oo leh OD260/OD280 agagaarka 1.8 ~ 1.9, iyo OD260/OD230＞2.0.
