TGuide Smart Magnetic Tissue DNA Kit
Lambarka buugaagta / baakadaha
| Bisad maya | ID | Tirada diyaarinta |
| 4995038 (taariko) | DP602-E | 96 |
| 4993547 (kartridge) | DP602-DE | 48 |
Hagaha xulashada Reagent ee TGuide S16
| Nuclei Acid | Tusaalaha Nooca | Magaca Alaabta | Cabbirka baakadka (diyaarinta) Kartuuji/ saxan | Bisadda maya. Kartuun/saanad |
| DNA | Nudaha Xoolaha | TGuide Smart Magnetic Tissue DNA Kit | 48/96 | 4993547/4995038 |
| DNA | Dhirta & Abuurka | Qalabka DNA-da ee Dhirta Birlabka leh ee TGuide | 48 | 4993548 |
| DNA | Ciid/ Saxaro | TGuide Smart Ciid / Qalabka DNA ee saxarada | 48 | 4993549 |
| DNA | Alaabooyinka jel & PCR | Qalabka Sifaynta DNA ee casriga ah ee TGuide | 48 | 4993550 |
| DNA | Dhiig dhan | TGuide Smart Dhiigga Genomic DNA Kit | 48/96 | 4993703/4995206 |
| DNA | Unug/Swab/Meelaha Qalalan, iwm | TGuide Smart Universal Qalabka DNA | 48/96 | 4993704/4995040 |
| RNA | Dhiiga / Unugyada | TGuide Smart Blood/Cutubka RNA Kit | 48/96 | 4993551/4995043 |
| RNA | Dhirta & Abuurka | TGuide Smart Magnetic Plant RNA Kit | 48 | 4993552 |
| DNA/RNA | Dheecaannada Unugyada Xorta ah (Serum, iwm) | TGuide Smart Viral DNA/RNA Kit | 48/96 | 4993702/4995207 |
hordhaca alaabta
Ka nadiifi DNA-da unugyada 10-50 mg in ka yar 50 daqiiqo.
DNA-da lagu sifeeyay xirmadan ayaa lagu dabaqi karaa codsiyo kala duwan oo xagga hoose ah, oo ay ku jiraan dheefshiidka, PCR, dhismaha maktabadda, isku-dhafka Koonfurta, iyo tijaabooyin kale.
Mugga elution dabacsan ayaa kuu ogolaanaya inaad sare u qaaddo fiirsashada ama wax-soo-saarka.
Astaamaha
Aad u fudud in la isticmaalo
Ma jirto shaqo tuubo oo dheeraad ah.
Mugga elution waxaa loo hagaajin karaa sida loo baahdo.
Rakibaadda iyo hawlgalku waxay u baahan yihiin tababar yar. Barnaamijyada horay loo rakibay, ka soo saar kartoonada, dooro borotokoolka oo socodsii tijaabadaada. Barnaamijyadu waa diyaar in la isticmaalo, sidoo kalena la beddeli karo.
Dib-u-dejiyayaal hore loo raray iyo agabka la tuuri karo
Maaraynta kiimikada waa la tirtiraa ilaa xadka ugu badan si loo xaqiijiyo badbaadada hawlgalka.
Natiijada Demo
Soo saaridda DNA-da xoolaha
Natiijada tijaabada: DNA-da unugyada xoolaha ee si toos ah u soo saartay TGuide S16 oo ku qalabaysan reagent TGuide Smart Magnetic Tissue DNA Kit (4993547) ayaa leh waxsoosaar sare iyo daahirnimo, taas oo u dhiganta DNA-da ay soo saartay nidaamka soo saarista lafdhabarta ku salaysan. Nadiifintu asal ahaan waa isku mid, oo leh OD260 / OD280 agagaarka 1.7 ~ 1.9 iyo OD260 / OD230> 1.7. Gebogebadii, TGuide S16 waxaa loo isticmaali karaa in laga soo saaro DNA-da genomic ee unugyada xayawaanka beddelka xalka soo saarista ku salaysan lafdhabarta.
Mugga soo dejinta: 2 μl.
Feejignaanta Gel Agarose: 1%. Electrophoresis 20 min at 6 V / cm;
M:TIANGEN Marker III
1-4: wadnaha 5-8: beeryarada 9-12: sambabada 13-16: kelyaha 17-20: maskaxda
Labada shay ee ugu horreeya ee unug kasta waxaa soo saaray xirmada soo saarista ku salaysan spin-column, iyo labadii ugu dambeeyay waxaa si toos ah u soo saaray TGuide S16.
Tusaalaha isha: CD-1 jiirarka
