Eukaryotic Mrna waa isku xigxiga-ngs
Astaamaha
● Qabsashada 'poly Mrna' ka hor diyaarinta maktabadda
● Diyaarinta maktabadda jihada ee MRNA ee u diyaargarowga si ay u suurto geliso helitaanka xogta taxanaha ee gaarka ah
Falanqaynta Bailinfeetic ee Muujinta Hante iyo qaab-dhismeedka Transcript
Faa'iidooyinka
●Khibrad ballaaran: Kooxdayadu waxay ka baaraandegay 600,000 oo muunado ah oo ka badan 600,000 oo muunado ah, oo ay soo saartay noocyo muunad oo kaladuwan ah sida dhaqamada unugyada, unugyada, iyo dareeraha jirka. Waxaan u soo qaadaneynaa khibrad aad u badan oo mashruuc kasta ah waxaana si guul leh u dhammeystireyyay in ka badan 100,000 mashruuc mashruuc Mrna-SEQ ah oo ku saabsan domain cilmi-baaris oo kala duwan.
●Xakamaynta tayada adag ee adag: Waxaan ka hirgelinaa qodobbada kontoroolka core ee dhammaan marxaladaha, tusaale ahaan muunadda iyo u diyaarinta maktabadda si ay u helaan isku xigxiga iyo bioinformatics. Kormeerkan la-socodkan ayaa hubiya gaarsiinta natiijooyinka tayo sare leh, taasoo kugu xaqiijinaysa in mashruucaagu uu ku jiro gacmahaaga lagu kalsoonaan karo.
●Faahfaahin dhameystiran: Waxaan u isticmaalnaa xog-ururinta badan si aan uga shaqeyno hidda-wadaha si kaladuwan loo muujiyey (DEG-yada) oo sameeya falanqaynta kobcinta. Qaabkan dhameystiran wuxuu siiyaa aragtiyo ku saabsan geedi socodka gacanta iyo molikalalar ee salka ku haya jawaabta shaashadda, hubinta in si buuxda loogu wargaliyo natiijooyinka mashruucaaga.
●Taageerada iibka ka dib: Balankeenna wuxuu ku fidsan yahay dhameystirka mashruuca ee leh muddo 3 bilood iibka iibka ah ka dib. Inta lagu jiro waqtigan, waxaan bixinaa dabagal dabagal mashruuc, gargaar dhibaatayn, iyo Q & SS & Kalfadhiyo si wax looga qabto wixii su'aalo ah ee la xiriira natiijooyinka.
Shuruudaha muunadda iyo dhalmada
| Maktabad | Istaraatiijiyadda isku xigta | Xogta lagu taliyay | Xakamaynta Tayada |
| Poly si fiican | Iftiimina PE150 Dnbseq-t7 | 6 -10 GB | Q3t85% |
Shuruudaha muunadda:
Nucleotatides:
| CODKA. (NG / μL) | Tirada (μg) | Daahir | Sharaf | |
| Maktabadda caadiga ah | 10 | ≥ 0.2 | OD260 / 280 = 1.7-2.5 OD260 / 230 = 0.5-2.5 Xadidan ama aan lahayn proteotiin ama wasakhda DNA ee lagu muujiyey jel. | Dhirta: Rin≥4.0; Xayawaanka: Rin≥4.5; 5.0≥28s / 18s≥1.0; Xaddidan ama kor u qaadis ah |
| Maktabadda Joogida | 10 | ≥ 0.2 | OD260 / 280 = 1.7-2.5 OD260 / 230 = 0.5-2.5 Xadidan ama aan lahayn proteotiin ama wasakhda DNA ee lagu muujiyey jel. | Dhirta: Rin≥4.0; Xayawaanka: Rin≥4.5; 5.0≥28s / 18s≥1.0; Xaddidan ama kor u qaadis ah |
Dhirta:
Xidid, asliga ah ama petal: 450 mg
Caleen ama abuur: 300 mg
Miraha: 1.2 g
●Xayawaanka:
Wadnaha ama xiidmaha: 300 mg
Viscera ama maskaxda: 240 mg
Murqaha: 450 mg
Lafaha, timaha ama maqaarka: 1g
● Arthropods:
Cayayaanka: 6g
Crustacea: 300 mg
Dhiiga oo dhan: 1 tuubbo
● Unugyada: 106 unugyada
Keenista muunad
Weelka: 2 ml centrifouge tuubada (foil tin tin laguma talinayo)
Calaamadaha muujinta: Kooxda + waxay ku daydaa tusaale ahaan a1, A2, A3; B1, B2, B3 ... ...
Shixnad:
1. Ice-ICE: Saamiyada ayaa loo baahan yahay in lagu buuxiyo bacaha oo lagu aasay baraf qalalan.
2. Tuubbooyinka miiska ee rna: Muunadaha rna waxaa lagu qallajin karaa tuubbooyinka xasilinta ee RNA (tusaale, rnastable®) oo lagu ridayaa heerkulka qolka.
Socodka shaqada adeegga
Naqshadeynta Tijaabada
Keenista muunad
Soo saarida RNA
Dhismaha Maktabadda
Kala xidowdo
Falanqaynta Xogta
Adeegyada ka dib
Bioinformatics
Eukaryotic Falanqaynta Falanqaynta Mrna
Bioinformatics
ØXakamaynta tayada tayada ee ceeriin
ØTixraaca isugeynta Genome
ØFalanqaynta Qaabka Qoraalka ah
ØTirada qoraalka
ØFalanqaynta muujinta kala duwan
ØFaahfaahinta iyo kobcinta
Tixraaca isugeynta Genome
Xogta fadhiga
Sawirka muunad iyo qiimeynta buugga bayoolajiga
Hiddo-wadaha si kala soocaya ee muujiyey (DEG-yada)
Faahfaahinta Shaqada ee Shaqada
Kobcinta shaqeysa ee DEGS
Baadh horumarka ay fududeeyaan BMKGene's's Eukaryotic's Eukaryat Scraquions Scraquions ee loo maro aruurinta daaqsinta daabacaadaha.
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