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DNBSEQ TABABARES BABRESS

Landseq Maktabadaha horay loo sameeyay oo loo soo bandhigay

Dnbseq, oo ay soo saartay MGI, waa tikniyoolajiyad NGS cusub oo ku guuleysatay inay hoos udhacdo kharashaadka isku-darka iyo kordhinta wax soo saarka. Diyaarinta maktabadaha DNBSEQ waxaa ku jira kala goynta DNA, diyaarinta SSDNA, iyo cod-weynaha wareejinta si loo helo DNA NANOBALLS (DNB). Kuwaani waxaa markaa lagu xiraa dusha sare oo dusha sare ah oo ay ku xigto isku-darka isku-darka-sarkaalka 'codadka' (CPAs). Tiknolojiyadda DNBSEQ waxay isku dareysaa faa'iidooyinka lahaanshaha sicirka qaladka cod-weynaha oo hooseeya iyadoo la adeegsanayo qaababka khaladaadka cufnaanta ee loo yaqaan 'nanoballs', taasoo dhalisay isku xirka iyo sax ahaanta.

Adeeggayaga Imtixaanada ee maktabadda ee laga sameeyay maktabadda ayaa u oggolaanaya macaamiisha inay u diyaargaroobaan maktabadaha isku-darka ee Iverina ee laga soo bilaabo ilo kala duwan (oo dhan), kuwaas oo loo rogo maktabadaha MGI, oo loo rogay maktabadaha MGI, Qaddarka xogta sare ee kharashaadka hoose.

Nala soo xiriir

Faahfaahinta adeegga

Natiijooyinka Demo

Astaamaha

●Malas:Mgi-dnbseq-t7

●Qaababka Jabwareenada:PE150

●Maktabadaha ku wareejinta maktabadda Iverina ilaaMgi:Awood u yeelashada isku xidhka tirooyinka xogta sare ee qiimaha jaban.

●Xakameynta Tayada ee Maktabadaha kahor intaan la isku xirin.

●Xisaabinta xogta Qc iyo dhalmada:Bixinta Warbixinta QC ee QC iyo Xogta cayriin ee qaab Degdeg ah ka dib markii lagu muujiyo burburin iyo shaandhaynta Q30 akhriska.

Faa'iidooyinka

●Kala-guurka adeegyada isku xirka:Macaamiilku wuxuu dooran karaa inuu isku xigto haadka ama xaddiga xogta.

●Soosaarka xogta sare:1500 GB / haad

●Gaarsiinta taxanaha warbixinta Qc:Iyada oo leh cabbir tayo leh, sax ahaanta macluumaadka iyo waxqabadka guud ee mashruuca isku xirka.

●Habka Isbeddelka Bisaanu:oo waqti gaaban u roon.

●Xakamaynta tayada adag ee adag: Waxaan u hirgelinnaa shuruudaha adag ee QC si loo dammaanad qaado gaarsiinta natiijooyinka tayo sare leh.

Shuruudaha muunadda

	Qaddarka xogta (x)	U fiirsashada (QPccr / NM)	Mug
Jadwalka qayb ahaan	X ≤ 10 gb	≥ 1Nm	25 μl
	10 GB <X ≤ 50 GB	≥ 2 nm	25 μl
	50 GB <X ≤ 100 GB	≥ 3 nm	25 μl
	X> 100 GB	≥ 4 nm
Hal haad	Nin kasta	≥ 1.5 nm / barkadda maktabadda	25 μl / Badda Maktabadda

Marka lagu daro feejignaanta iyo wadarta lacagta, qaabka ugu habboon ee ugu habboon ayaa sidoo kale loo baahan yahay.

XUSUUSNOW: Hay'adaha haadka ee maktabadaha kala duwanaanshaha ee hooseeya ayaa u baahan phix Spike-gudaha si loo hubiyo in salka adag ee salka lagu waco.

Waxaan kugula talineynaa inaad soo geliso maktabadaha horay loo siiyey sida muunado ah. Hadaad ubaahantahay BMKGE si aad u qabato maktabad, fadlan tixraac

Shuruudaha Maktabadda ee Qeybta Qaadashada Jang ee Jang.

Cabbirka maktabadda (Khariidadda ugu sarreysa)

Ugu sarreysa waa inay ahaataa 300-450 BP.

Maktabadaha waa inay lahaadaan hal ugu sarreeya oo ugu sarreeya, majiraan aqal faddarenimo iyo kuwa sareeya

Shaqada adeegga

Hore: HI-C salka ku saleysan is-dhexgalka korofo

Xiga: Bmkmuru s3000_spatial transcritom

Maktabadda QC Warbixinta

Warbixin ku saabsan tayada maktabadda waxaa la bixiyaa ka hor intaadan isku xigxin, qiimeynta qaddarka maktabadda, iyo kala-qaybsashada.

Isku xirka Warbixinta QC

Shaxda 1. Tirakoob ku saabsan xogta taxanaha ah.

Aqoonsi muunad ah	Bmkid	Cayriin ayaa akhrinaya	Xogta cayriin (bp)	Nadiifi akhrinta (%)	Q20 (%)	Q30 (%)	GC (%)
C_01	Bmk_01	22,870,120	6,861,036,000	96.48	99.14	94.85	36.67
C_02	Bmk_02	14,717,867	4,415,360,100	96.00	98.95	93.89	37.08