De novo Bacterial Genome Assembly
Waxaan bixinaa adeeg ururinta genome bakteeriyada dhamaystiran, dammaanad 0 nusqaamaha. Tani waxay suurtogal u tahay iyada oo la isku darayo tignoolajiyada isku xigxiga ee akhriska dheer, sida Nanopore iyo PacBio ee isu imaatinka iyo akhrinta gaaban ee Illumina si loo ansixiyo kulanka iyo sixitaanka qaladka ee akhrinta ONT. Adeegeena wuxuu bixiyaa qulqulka shaqada bioinformatic oo dhammaystiran oo ka imanaya isu imaatinka, sharraxaadda shaqaynaysa, iyo falanqaynta bioinformatic ee horumarsan, iyada oo buuxinaysa yoolalka cilmi-baarista gaarka ah. Adeegani wuxuu awood u siinayaa horumarinta genome tixraacyada saxda ah ee cilmi-baarista hidde-sideyaasha kala duwan. Intaa waxaa dheer, waxay aasaas u tahay codsiyada sida hagaajinta culeyska, injineernimada hidaha, iyo horumarinta tignoolajiyada microbial, hubinta la isku halayn karo oo aan faraq lahayn xogta genomic muhiim u ah horumarinta fikradaha sayniska iyo hal-abuurnimada bayolojiyada.
Tilmaamaha Adeegga
● Iyada oo leh laba ikhtiyaar oo suurtagal ah oo lagu kala dooran karo iyadoo ku xiran heerka la rabo ee dhammaystirnaanta genome.
● Xulashada Genome-ka Qabyada ah: Isku xigxiga akhris-gaaban ee Illumina NovaSeq PE150.
● Dhammaystir 0 farqiga u dhexeeya genome.
● Akhriska dheer ee taxanaha Nanopore PromethION 48 ama PacBio Revio ee isu imaatinka genome.
● Akhriska-gaaban ee taxanaha Illumina NovaSeq ee ansaxinta genome-ga iyo sixitaanka khaladka (Nanopore) ama in la dhaliyo genome qabyo ah.
Faa'iidooyinka Adeegga
●Genom-gaab eber ah waa la dammaanad qaadayTani waxay sabab u tahay isku-dhafka Illumina taxane ah oo leh taxane akhris dheer (Nanopore ama PacBio).
●Socodka Shaqada Bioinformatics oo Dhameystiran:Tan waxa ku jira isu-ururinta genome-ga iyo odoroska curiyeyaasha genomic ee badan, sharraxaadda hidda-wadaha shaqaynaysa, iyo muuqaallada genome-ka sida goob-qoreedka Circos.
●Khibrad Balaadhan: Iyadoo in ka badan 20,000 genomes microbial la ururiyey, BMKGENE waxay keentaa in ka badan toban sano oo waayo-aragnimo ah, koox falanqaynta xirfad sare leh, content dhamaystiran, iyo taageero iibka ka dib aad u fiican.
●Taageerada Iibka Kadib:Ballanqaadkayaga waxa uu dhaafsiisan yahay dhamaystirka mashruuca oo leh 3 bilood oo adeega iibka kadib. Inta lagu jiro wakhtigan, waxaanu bixinaa dabagalka mashruuca, caawinta cilad-raadinta, iyo fadhiyada Q&A si wax looga qabto wixii su'aalo ah ee la xidhiidha natiijooyinka.
●Xeelado isku xigxiga oo badan ayaa jira:Ujeedooyinka cilmi-baarista ee kala duwan iyo shuruudaha dhammaystirka genome.
Tilmaamaha Adeegga
| Adeegga | Istaraatiijiyada Taxanaha |
| Qabyada Genome | Illumina PE150 100x |
| 0 Gap Genome | Nanopore 100x + Illumina PE150 100x Or Pacbio HiFi 30x + Illumina PE150 100x (ikhtiyaar) |
Tusaalaha Shuruudaha:
| Xooga saarida (ng/µL) | Tirada guud (µg) | Mugga (µL) | OD260/280 | OD260/230 | |
| PacBio | ≥20 | ≥1.2 | ≥20 | 1.7-2.2 | ≥1.0 |
| Nanopore | ≥40 | ≥2 | ≥20 | 1.7-2.2 | 1.0-3.0 |
| Illumina | ≥1 | ≥0.06 | ≥20 | - | - |
Bakteeriyada: ≥3.5x1010 unugyo
Socodka Shaqada Adeegga
Tusaalaha keenista
Dhismaha maktabadda
Isku xigxiga
Falanqaynta xogta
Adeegyada iibka kadib
Waxa ku jira Falanqaynta soo socota:
● Isku xigxiga Xakamaynta Tayada Xogta
● Golaha genome
● Falanqaynta Qaybaha Genome: Saadaasha CDS iyo xubno badan oo genomic ah
● Sharaxaad shaqaynaysa oo wadata xog ururin guud oo badan (GO, KEGG, iwm) iyo kayd xogeed horumarsan (CARD, VFDB, iwm)
● Aragtida Genome
Waxaanu ku bixinaa genome-ka qaab fasta ah oo diyaarsan oo la heli karo iyo faylka annotation genome (gff).
Tilmaamaha hidda-socodka - GO
Tilmaamaha hidda-wadaha - karbohaydraytyada CAZY
Aragtida genome - Sawirka Circos
Sahami horumarka ay fududaysay BMKGene's bakteeriyada genome-ga adeegyada isu-ururinta daabacaadyada.
Dai, W. iyo al. (2023) 'Daahfurka Bacteroides lebbiska F18-22 oo ah Bakteeriya badbaado iyo Novel Probiotic ah oo loogu talagalay daawaynta colitis calooleedka ee mindhicirka bini'aadamka ee caafimaadka qaba',Joornaalka Caalamiga ah ee Cilmiga Molecular, 24(19), b. 14669. doi: 10.3390/IJMS241914669/S1.
Kang, Q. iyo al. (2021) 'Proteus mirabilis-ka-go'doominta u adkaysta badan ee sidada blaOXA-1 iyo blaNDM-1 ee duurjoogta Shiinaha: kordhinta khatarta caafimaadka dadweynaha',Isku-dhafka Xayawaanka, 16(6), bogga 798–809. doi: 10.1111/1749-4877.12510.
Wang, TT iyo al. (2017) 'Dhammaan taxanaha genome ee endophyte Bacillus flexus KLBMP 4941 waxay daaha ka qaadaysaa habka kor u qaadida koritaanka dhirta iyo aasaaska hiddaha ee dulqaadka milixda',Wargeyska Biotechnology, 260, bogga 38-41. doi: 10.1016/J.JBIOTEC.2017.09.001.
Wang, X. iyo al. (2021) 'Blasmids Resistance Resistance Multiple-Replicon Plasmids of Klebsiella Dhexdhexaadinta Faafinta Balaadhan ee Hidde-yaasha Kahortaga',Xuduudaha Microbiology, 12, p. 754931. doi: 10.3389/FMICB.2021.754931/BIBTEX.
